Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation
Abstract The amygdala is modulated by dopaminergic and cholinergic neurotransmission, and this modulation is altered in mood disorders. Therefore, this study was designed to evaluate the presence/absence of quantitative alterations in the expression of main dopaminergic and cholinergic markers in th...
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| Format: | Article |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28069-2 |
| _version_ | 1797945917578412032 |
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| author | Daniel Kalinowski Krystyna Bogus-Nowakowska Anna Kozłowska Maciej Równiak |
| author_facet | Daniel Kalinowski Krystyna Bogus-Nowakowska Anna Kozłowska Maciej Równiak |
| author_sort | Daniel Kalinowski |
| collection | DOAJ |
| description | Abstract The amygdala is modulated by dopaminergic and cholinergic neurotransmission, and this modulation is altered in mood disorders. Therefore, this study was designed to evaluate the presence/absence of quantitative alterations in the expression of main dopaminergic and cholinergic markers in the amygdala of mice with oestrogen receptor β (ERβ) knock-out which exhibit increased anxiety, using immunohistochemistry and quantitative methods. Such alterations could either contribute to increased anxiety or be a compensatory mechanism for reducing anxiety. The results show that among dopaminergic markers, the expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and dopamine D2-like receptor (DA2) is significantly elevated in the amygdala of mice with ERβ deprivation when compared to matched controls, whereas the content of dopamine D1-like receptor (DA1) is not altered by ERβ knock-out. In the case of cholinergic markers, muscarinic acetylcholine type 1 receptor (AChRM1) and alpha-7 nicotinic acetylcholine receptor (AChRα7) display overexpression while the content of acetylcholinesterase (AChE) and vesicular acetylcholine transporter (VAChT) remains unchanged. In conclusion, in the amygdala of ERβ knock-out female the dopaminergic and cholinergic signalling is altered, however, to determine the exact role of ERβ in the anxiety-related behaviour further studies are required. |
| first_indexed | 2024-04-10T21:02:42Z |
| format | Article |
| id | doaj.art-fb1fbcb6581f4b46877eb11f7d160ac0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T21:02:42Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fb1fbcb6581f4b46877eb11f7d160ac02023-01-22T12:14:06ZengNature PortfolioScientific Reports2045-23222023-01-0113111810.1038/s41598-023-28069-2Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivationDaniel Kalinowski0Krystyna Bogus-Nowakowska1Anna Kozłowska2Maciej Równiak3Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in OlsztynDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynAbstract The amygdala is modulated by dopaminergic and cholinergic neurotransmission, and this modulation is altered in mood disorders. Therefore, this study was designed to evaluate the presence/absence of quantitative alterations in the expression of main dopaminergic and cholinergic markers in the amygdala of mice with oestrogen receptor β (ERβ) knock-out which exhibit increased anxiety, using immunohistochemistry and quantitative methods. Such alterations could either contribute to increased anxiety or be a compensatory mechanism for reducing anxiety. The results show that among dopaminergic markers, the expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and dopamine D2-like receptor (DA2) is significantly elevated in the amygdala of mice with ERβ deprivation when compared to matched controls, whereas the content of dopamine D1-like receptor (DA1) is not altered by ERβ knock-out. In the case of cholinergic markers, muscarinic acetylcholine type 1 receptor (AChRM1) and alpha-7 nicotinic acetylcholine receptor (AChRα7) display overexpression while the content of acetylcholinesterase (AChE) and vesicular acetylcholine transporter (VAChT) remains unchanged. In conclusion, in the amygdala of ERβ knock-out female the dopaminergic and cholinergic signalling is altered, however, to determine the exact role of ERβ in the anxiety-related behaviour further studies are required.https://doi.org/10.1038/s41598-023-28069-2 |
| spellingShingle | Daniel Kalinowski Krystyna Bogus-Nowakowska Anna Kozłowska Maciej Równiak Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation Scientific Reports |
| title | Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| title_full | Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| title_fullStr | Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| title_full_unstemmed | Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| title_short | Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| title_sort | dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation |
| url | https://doi.org/10.1038/s41598-023-28069-2 |
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