Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli
In order to make piglet diets more effective, it is necessary to investigate effective methods for breaking down xylan in cereal. The objective of this study was to determine the effects of dietary stimbiotic (STB) supplementation on growth performance, intestinal morphology, immune response and int...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1187002/full |
| _version_ | 1797777386717052928 |
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| author | Dongcheol Song Jihwan Lee Woogi Kwak Hanjin Oh Seyeon Chang Jaewoo An Hyunah Cho Sehyun Park Kyeongho Jeon Jinho Cho |
| author_facet | Dongcheol Song Jihwan Lee Woogi Kwak Hanjin Oh Seyeon Chang Jaewoo An Hyunah Cho Sehyun Park Kyeongho Jeon Jinho Cho |
| author_sort | Dongcheol Song |
| collection | DOAJ |
| description | In order to make piglet diets more effective, it is necessary to investigate effective methods for breaking down xylan in cereal. The objective of this study was to determine the effects of dietary stimbiotic (STB) supplementation on growth performance, intestinal morphology, immune response and intestinal microbiota in weaned piglets. A total of 24 (Duroc × Yorkshire × Landrace) weaned pigs (initial body weight of 8.01 ± 0.38 kg and 28 ± 3 d old), were assigned to 4 treatments with 6 replicates per treatment. Pigs were housed in individual pens for 17 days, including 5 days adaption period and 12 days after the first Escherichia coli (E. coli) challenge. The experiment was conducted in a 2 × 2 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) and two levels of STB (0 and 0.5 g/kg diet). Supplementations of STB 0.5 g/kg improved the gain to feed ratio (G:F) (P < 0.05) in piglets challenged with shiga toxigenic E. coli (STEC). STB supplementation decreased (P < 0.05) white blood cells, neutrophils, lymphocytes, and expression levels of tumor necrosis factor-alpha and interleukin-6. Supplementation of STB improved (P < 0.05) the lymphocytes and neutrophils in piglets challenged with STEC on 12 dpi. Supplementation of STB also improved (P < 0.05) the villus height to-crypt depth ratio of ileum in piglets challenged with STEC. Supplementation of STB increased (P < 0.05) the expression levels of claudin-1 of ileum. In genus level, supplementation of STB increased (P < 0.001) the abundance of Prevotella compared to non-supplementation of STB groups in pre-inoculation period. Also, supplementation of STB decreased (P < 0.05) the abundance of Faecalibacterium and Eubacterium_coprostanoligenes_group compared to non-supplementation of STB groups in post-inoculation period. In phylum level, supplementation of STB increased (P < 0.05) the abundance of Desulfobacterota and Fibrobacterota in pre-inoculation period. E. coli challenge increased the abundance of Fibrobacterota compared to non-challenged group in post-inoculation period. In conclusion, these findings indicated that STB supplementation could alleviate a decrease of the performance, immune response, and inflammatory response in piglets induced by the STEC challenge. |
| first_indexed | 2024-03-12T23:03:17Z |
| format | Article |
| id | doaj.art-fb221823d5c443d4ae34008ed8d9b707 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T23:03:17Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-fb221823d5c443d4ae34008ed8d9b7072023-07-19T07:51:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-07-011010.3389/fvets.2023.11870021187002Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coliDongcheol Song0Jihwan Lee1Woogi Kwak2Hanjin Oh3Seyeon Chang4Jaewoo An5Hyunah Cho6Sehyun Park7Kyeongho Jeon8Jinho Cho9Department of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Poultry Science, University of Georgia (UGA), Athens, GA, United StatesDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaDepartment of Animal Science, Chungbuk National University, Cheongju, Republic of KoreaIn order to make piglet diets more effective, it is necessary to investigate effective methods for breaking down xylan in cereal. The objective of this study was to determine the effects of dietary stimbiotic (STB) supplementation on growth performance, intestinal morphology, immune response and intestinal microbiota in weaned piglets. A total of 24 (Duroc × Yorkshire × Landrace) weaned pigs (initial body weight of 8.01 ± 0.38 kg and 28 ± 3 d old), were assigned to 4 treatments with 6 replicates per treatment. Pigs were housed in individual pens for 17 days, including 5 days adaption period and 12 days after the first Escherichia coli (E. coli) challenge. The experiment was conducted in a 2 × 2 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) and two levels of STB (0 and 0.5 g/kg diet). Supplementations of STB 0.5 g/kg improved the gain to feed ratio (G:F) (P < 0.05) in piglets challenged with shiga toxigenic E. coli (STEC). STB supplementation decreased (P < 0.05) white blood cells, neutrophils, lymphocytes, and expression levels of tumor necrosis factor-alpha and interleukin-6. Supplementation of STB improved (P < 0.05) the lymphocytes and neutrophils in piglets challenged with STEC on 12 dpi. Supplementation of STB also improved (P < 0.05) the villus height to-crypt depth ratio of ileum in piglets challenged with STEC. Supplementation of STB increased (P < 0.05) the expression levels of claudin-1 of ileum. In genus level, supplementation of STB increased (P < 0.001) the abundance of Prevotella compared to non-supplementation of STB groups in pre-inoculation period. Also, supplementation of STB decreased (P < 0.05) the abundance of Faecalibacterium and Eubacterium_coprostanoligenes_group compared to non-supplementation of STB groups in post-inoculation period. In phylum level, supplementation of STB increased (P < 0.05) the abundance of Desulfobacterota and Fibrobacterota in pre-inoculation period. E. coli challenge increased the abundance of Fibrobacterota compared to non-challenged group in post-inoculation period. In conclusion, these findings indicated that STB supplementation could alleviate a decrease of the performance, immune response, and inflammatory response in piglets induced by the STEC challenge.https://www.frontiersin.org/articles/10.3389/fvets.2023.1187002/fullstimbioticimmune responsegut healthE. coliintestinal microbiota |
| spellingShingle | Dongcheol Song Jihwan Lee Woogi Kwak Hanjin Oh Seyeon Chang Jaewoo An Hyunah Cho Sehyun Park Kyeongho Jeon Jinho Cho Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli Frontiers in Veterinary Science stimbiotic immune response gut health E. coli intestinal microbiota |
| title | Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli |
| title_full | Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli |
| title_fullStr | Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli |
| title_full_unstemmed | Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli |
| title_short | Effects of stimbiotic supplementation on gut health, immune response, and intestinal microbiota in weaned piglets challenged with E. coli |
| title_sort | effects of stimbiotic supplementation on gut health immune response and intestinal microbiota in weaned piglets challenged with e coli |
| topic | stimbiotic immune response gut health E. coli intestinal microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1187002/full |
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