Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species
The endogenous microbiome of healthy individuals in oral cavities is diverse, representing over 700 bacterial species. Imbalance in oral and gut microbiome composition and associated gene expression has been linked to different forms of hematological (blood) cancers. Our objective is to compare oral...
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MDPI AG
2020-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/12/4068 |
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| author | Jean-Luc C. Mougeot Micaela F. Beckman Holden C. Langdon Michael T. Brennan Farah Bahrani Mougeot |
| author_facet | Jean-Luc C. Mougeot Micaela F. Beckman Holden C. Langdon Michael T. Brennan Farah Bahrani Mougeot |
| author_sort | Jean-Luc C. Mougeot |
| collection | DOAJ |
| description | The endogenous microbiome of healthy individuals in oral cavities is diverse, representing over 700 bacterial species. Imbalance in oral and gut microbiome composition and associated gene expression has been linked to different forms of hematological (blood) cancers. Our objective is to compare oral microbiome profiles of patients with blood cancers (BC group: N = 39 patients, n = 124 oral samples) to those of healthy control subjects (HC group: N = 27 subjects, n = 100 oral samples). Saliva samples and swabs of buccal mucosa, supragingival plaque, and tongue were collected from blood cancer patients and healthy controls. Next-generation sequencing (16S-rRNA gene V3–V4 region) was used to determine the relative abundance of bacterial taxa present at the genus and species levels. Differences in oral microbiome <i>beta</i>-diversity were determined using multivariate permutational analysis of variance (PERMANOVA). Linear discriminant analysis (LDA) effect size (LEfSe) analysis was performed to identify differentiating bacterial taxa in pairwise comparisons. The PATRIC<sub>v3.6.7</sub> online tool was used to determine the predominance of potential pathogenicity in the BC group. The oral microbiome <i>beta</i>-diversities of the BC and HC groups differed and corresponded to a reduced <i>alpha</i>-diversity in the BC group. LEfSe analysis showed significant LDA scores for <i>Actinomyces</i> and <i>Rothia</i> spp., differentiating the BC group from the HC group. In silico analysis using PATRIC<sub>v3.6.7</sub> demonstrated that the groups of bacteria possessing traits of “antibiotic resistance”, “oral pathogen”, and “virulence” was enriched in the BC group. Although 56% of the BC patients received antibiotics within two weeks of the oral bacterial sampling, <i>Actinomyces genus</i> remained the top differentiating feature in the BC group regardless of the administration of antibiotics, while <i>Rothia dentocariosa</i> was detected as the top differentiating feature in the BC patients who did not receive antibiotics, but not in those who received antibiotics. Further investigation is needed to better understand the interactions of certain oral species with the host immune system to better characterize clinically relevant associations with hematological cancers. |
| first_indexed | 2024-03-10T14:00:38Z |
| format | Article |
| id | doaj.art-fb2427e6da884cbf8efce29d0d5b348c |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T14:00:38Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-fb2427e6da884cbf8efce29d0d5b348c2023-11-21T01:11:57ZengMDPI AGJournal of Clinical Medicine2077-03832020-12-01912406810.3390/jcm9124068Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> SpeciesJean-Luc C. Mougeot0Micaela F. Beckman1Holden C. Langdon2Michael T. Brennan3Farah Bahrani Mougeot4Carolinas Medical Center-Atrium Health, 1000 Blythe Blvd, Charlotte, NC 28203, USACarolinas Medical Center-Atrium Health, 1000 Blythe Blvd, Charlotte, NC 28203, USACarolinas Medical Center-Atrium Health, 1000 Blythe Blvd, Charlotte, NC 28203, USACarolinas Medical Center-Atrium Health, 1000 Blythe Blvd, Charlotte, NC 28203, USACarolinas Medical Center-Atrium Health, 1000 Blythe Blvd, Charlotte, NC 28203, USAThe endogenous microbiome of healthy individuals in oral cavities is diverse, representing over 700 bacterial species. Imbalance in oral and gut microbiome composition and associated gene expression has been linked to different forms of hematological (blood) cancers. Our objective is to compare oral microbiome profiles of patients with blood cancers (BC group: N = 39 patients, n = 124 oral samples) to those of healthy control subjects (HC group: N = 27 subjects, n = 100 oral samples). Saliva samples and swabs of buccal mucosa, supragingival plaque, and tongue were collected from blood cancer patients and healthy controls. Next-generation sequencing (16S-rRNA gene V3–V4 region) was used to determine the relative abundance of bacterial taxa present at the genus and species levels. Differences in oral microbiome <i>beta</i>-diversity were determined using multivariate permutational analysis of variance (PERMANOVA). Linear discriminant analysis (LDA) effect size (LEfSe) analysis was performed to identify differentiating bacterial taxa in pairwise comparisons. The PATRIC<sub>v3.6.7</sub> online tool was used to determine the predominance of potential pathogenicity in the BC group. The oral microbiome <i>beta</i>-diversities of the BC and HC groups differed and corresponded to a reduced <i>alpha</i>-diversity in the BC group. LEfSe analysis showed significant LDA scores for <i>Actinomyces</i> and <i>Rothia</i> spp., differentiating the BC group from the HC group. In silico analysis using PATRIC<sub>v3.6.7</sub> demonstrated that the groups of bacteria possessing traits of “antibiotic resistance”, “oral pathogen”, and “virulence” was enriched in the BC group. Although 56% of the BC patients received antibiotics within two weeks of the oral bacterial sampling, <i>Actinomyces genus</i> remained the top differentiating feature in the BC group regardless of the administration of antibiotics, while <i>Rothia dentocariosa</i> was detected as the top differentiating feature in the BC patients who did not receive antibiotics, but not in those who received antibiotics. Further investigation is needed to better understand the interactions of certain oral species with the host immune system to better characterize clinically relevant associations with hematological cancers.https://www.mdpi.com/2077-0383/9/12/4068oral microbiomehematological or blood cancersnext-generation 16S sequencingLEfSe biomarkers<i>Actinomyces</i> |
| spellingShingle | Jean-Luc C. Mougeot Micaela F. Beckman Holden C. Langdon Michael T. Brennan Farah Bahrani Mougeot Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species Journal of Clinical Medicine oral microbiome hematological or blood cancers next-generation 16S sequencing LEfSe biomarkers <i>Actinomyces</i> |
| title | Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species |
| title_full | Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species |
| title_fullStr | Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species |
| title_full_unstemmed | Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species |
| title_short | Oral Microbiome Signatures in Hematological Cancers Reveal Predominance of <i>Actinomyces</i> and <i>Rothia</i> Species |
| title_sort | oral microbiome signatures in hematological cancers reveal predominance of i actinomyces i and i rothia i species |
| topic | oral microbiome hematological or blood cancers next-generation 16S sequencing LEfSe biomarkers <i>Actinomyces</i> |
| url | https://www.mdpi.com/2077-0383/9/12/4068 |
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