Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis
Abstract Certain probiotic species of lactic acid bacteria, especially Lactobacillus plantarum, regulate bacteriocin synthesis through quorum sensing (QS) systems. In this study, we aimed to investigate the luxS-mediated molecular mechanisms of QS during bacteriocin synthesis by L. plantarum KLDS1.0...
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Nature Portfolio
2017-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-13231-4 |
| _version_ | 1819040006499467264 |
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| author | Fang-Fang Jia Xue-Hui Pang De-Quan Zhu Zong-Tao Zhu Si-Rui Sun Xiang-Chen Meng |
| author_facet | Fang-Fang Jia Xue-Hui Pang De-Quan Zhu Zong-Tao Zhu Si-Rui Sun Xiang-Chen Meng |
| author_sort | Fang-Fang Jia |
| collection | DOAJ |
| description | Abstract Certain probiotic species of lactic acid bacteria, especially Lactobacillus plantarum, regulate bacteriocin synthesis through quorum sensing (QS) systems. In this study, we aimed to investigate the luxS-mediated molecular mechanisms of QS during bacteriocin synthesis by L. plantarum KLDS1.0391. In the absence of luxS, the ‘spot-on-the-lawn’ method showed that the bacteriocin production by L. plantarum KLDS1.0391 significantly decreased upon co-cultivation with L. helveticus KLDS1.9207 (P < 0.01) but did not change significantly when mono-cultivated. Furthermore, liquid chromatography-electrospray ionization tandem mass spectrometry analysis showed that, as a response to luxS deletion, L. plantarum KLDS1.0391 altered the expression level of proteins involved in carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. In particular, the sensor histidine kinase AgrC (from the two-component system, LytTR family) was expressed differently between the luxS mutant and the wild-type strain during co-cultivation, whereas no significant differences in proteins related to bacteriocin biosynthesis were found upon mono-cultivation. In summary, we found that the production of bacteriocin was regulated by carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. Furthermore, our results demonstrate the role of luxS-mediated molecular mechanisms in bacteriocin production. |
| first_indexed | 2024-12-21T09:02:14Z |
| format | Article |
| id | doaj.art-fb2522046adf42f89dfb609ebbbe3943 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:02:14Z |
| publishDate | 2017-10-01 |
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| series | Scientific Reports |
| spelling | doaj.art-fb2522046adf42f89dfb609ebbbe39432022-12-21T19:09:27ZengNature PortfolioScientific Reports2045-23222017-10-017111410.1038/s41598-017-13231-4Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysisFang-Fang Jia0Xue-Hui Pang1De-Quan Zhu2Zong-Tao Zhu3Si-Rui Sun4Xiang-Chen Meng5Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural UniversityAbstract Certain probiotic species of lactic acid bacteria, especially Lactobacillus plantarum, regulate bacteriocin synthesis through quorum sensing (QS) systems. In this study, we aimed to investigate the luxS-mediated molecular mechanisms of QS during bacteriocin synthesis by L. plantarum KLDS1.0391. In the absence of luxS, the ‘spot-on-the-lawn’ method showed that the bacteriocin production by L. plantarum KLDS1.0391 significantly decreased upon co-cultivation with L. helveticus KLDS1.9207 (P < 0.01) but did not change significantly when mono-cultivated. Furthermore, liquid chromatography-electrospray ionization tandem mass spectrometry analysis showed that, as a response to luxS deletion, L. plantarum KLDS1.0391 altered the expression level of proteins involved in carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. In particular, the sensor histidine kinase AgrC (from the two-component system, LytTR family) was expressed differently between the luxS mutant and the wild-type strain during co-cultivation, whereas no significant differences in proteins related to bacteriocin biosynthesis were found upon mono-cultivation. In summary, we found that the production of bacteriocin was regulated by carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. Furthermore, our results demonstrate the role of luxS-mediated molecular mechanisms in bacteriocin production.https://doi.org/10.1038/s41598-017-13231-4 |
| spellingShingle | Fang-Fang Jia Xue-Hui Pang De-Quan Zhu Zong-Tao Zhu Si-Rui Sun Xiang-Chen Meng Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis Scientific Reports |
| title | Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis |
| title_full | Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis |
| title_fullStr | Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis |
| title_full_unstemmed | Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis |
| title_short | Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis |
| title_sort | role of the luxs gene in bacteriocin biosynthesis by lactobacillus plantarum klds1 0391 a proteomic analysis |
| url | https://doi.org/10.1038/s41598-017-13231-4 |
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