Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes
Tumor-derived extracellular vesicles (TD-EVs) have active roles as cancer hallmark enablers. EVs RNA of epithelial and stromal cells carry information that facilitates the communication processes that contribute to oncological progression, so the objective of this work was to validate by RT-PCR the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3578 |
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| author | Begoña O. Alen Lara Sofía Estévez-Pérez María Otero Alén Saioa Domínguez Hormaetxe Laureano Simón Ángel Concha |
| author_facet | Begoña O. Alen Lara Sofía Estévez-Pérez María Otero Alén Saioa Domínguez Hormaetxe Laureano Simón Ángel Concha |
| author_sort | Begoña O. Alen |
| collection | DOAJ |
| description | Tumor-derived extracellular vesicles (TD-EVs) have active roles as cancer hallmark enablers. EVs RNA of epithelial and stromal cells carry information that facilitates the communication processes that contribute to oncological progression, so the objective of this work was to validate by RT-PCR the presence of epithelial (<i>KRT19</i>; <i>CEA</i>) and stromal (<i>COL1A2</i>; <i>COL11A1</i>) markers in RNA of plasmatic EVs in healthy and diverse-malignancy patients for the development of a non-invasive cancer diagnosis system using liquid biopsy. Ten asymptomatic controls and 20 cancer patients were included in the study, and results showed that the isolated plasmatic EVs by scanning transmission electron microscopy (STEM) andBiomedical Research Institute A Coruña nanoparticle tracking analysis (NTA) contained most exosome structures with also a considerable percentage of microvesicles. No differences were found in concentration and size distribution between the two cohorts of patients, but significant gene expression in epithelial and mesenchymal markers between healthy donors and patients with active oncological disease was shown. Results of quantitative RT-PCR are solid and reliable for <i>KRT19</i>, <i>COL1A2</i>, and <i>COL11A1</i>, so the analysis of RNA extracted from TD-EVs could be a correct approach to develop a diagnostic tool in oncological processes. |
| first_indexed | 2024-03-11T08:43:33Z |
| format | Article |
| id | doaj.art-fb29ed0a587b4c268b8a06bbce0bf903 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:43:33Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fb29ed0a587b4c268b8a06bbce0bf9032023-11-16T21:01:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244357810.3390/ijms24043578Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic ProcessesBegoña O. Alen0Lara Sofía Estévez-Pérez1María Otero Alén2Saioa Domínguez Hormaetxe3Laureano Simón4Ángel Concha5Molecular Biology Area, Department of Anatomical Pathology, Biomedical Research Institute A Coruña (INIBIC), University Hospital Complex A Coruña, 15006 A Coruña, SpainMolecular Biology Area, Department of Anatomical Pathology, Biomedical Research Institute A Coruña (INIBIC), University Hospital Complex A Coruña, 15006 A Coruña, SpainSantiago de Compostela Health Research Institute (IDIS), University Hospital Complex Santiago de Compostela, 15706 Santiago de Compostela, SpainOncomatryx Biopharma, 48160 Derio, SpainOncomatryx Biopharma, 48160 Derio, SpainMolecular Biology Area, Department of Anatomical Pathology, Biomedical Research Institute A Coruña (INIBIC), University Hospital Complex A Coruña, 15006 A Coruña, SpainTumor-derived extracellular vesicles (TD-EVs) have active roles as cancer hallmark enablers. EVs RNA of epithelial and stromal cells carry information that facilitates the communication processes that contribute to oncological progression, so the objective of this work was to validate by RT-PCR the presence of epithelial (<i>KRT19</i>; <i>CEA</i>) and stromal (<i>COL1A2</i>; <i>COL11A1</i>) markers in RNA of plasmatic EVs in healthy and diverse-malignancy patients for the development of a non-invasive cancer diagnosis system using liquid biopsy. Ten asymptomatic controls and 20 cancer patients were included in the study, and results showed that the isolated plasmatic EVs by scanning transmission electron microscopy (STEM) andBiomedical Research Institute A Coruña nanoparticle tracking analysis (NTA) contained most exosome structures with also a considerable percentage of microvesicles. No differences were found in concentration and size distribution between the two cohorts of patients, but significant gene expression in epithelial and mesenchymal markers between healthy donors and patients with active oncological disease was shown. Results of quantitative RT-PCR are solid and reliable for <i>KRT19</i>, <i>COL1A2</i>, and <i>COL11A1</i>, so the analysis of RNA extracted from TD-EVs could be a correct approach to develop a diagnostic tool in oncological processes.https://www.mdpi.com/1422-0067/24/4/3578extracellular vesiclesepithelial biomarkermesenchymal biomarkercancer diagnosisliquid biopsy |
| spellingShingle | Begoña O. Alen Lara Sofía Estévez-Pérez María Otero Alén Saioa Domínguez Hormaetxe Laureano Simón Ángel Concha Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes International Journal of Molecular Sciences extracellular vesicles epithelial biomarker mesenchymal biomarker cancer diagnosis liquid biopsy |
| title | Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes |
| title_full | Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes |
| title_fullStr | Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes |
| title_full_unstemmed | Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes |
| title_short | Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes |
| title_sort | expression of epithelial and mesenchymal markers in plasmatic extracellular vesicles as a diagnostic tool for neoplastic processes |
| topic | extracellular vesicles epithelial biomarker mesenchymal biomarker cancer diagnosis liquid biopsy |
| url | https://www.mdpi.com/1422-0067/24/4/3578 |
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