Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca
A water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-08-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/16/8/281 |
| _version_ | 1828394766984806400 |
|---|---|
| author | Peipei Li Songsong Wen Kunlai Sun Yuqin Zhao Yin Chen |
| author_facet | Peipei Li Songsong Wen Kunlai Sun Yuqin Zhao Yin Chen |
| author_sort | Peipei Li |
| collection | DOAJ |
| description | A water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa. Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR). The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues. The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→. Most (70%) of the rhamnose was sulfated. UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→. UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro. This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan. |
| first_indexed | 2024-12-10T08:00:18Z |
| format | Article |
| id | doaj.art-fb2bec8967344d14849e8a5756db72cc |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T08:00:18Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-fb2bec8967344d14849e8a5756db72cc2022-12-22T01:56:48ZengMDPI AGMarine Drugs1660-33972018-08-0116828110.3390/md16080281md16080281Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flaccaPeipei Li0Songsong Wen1Kunlai Sun2Yuqin Zhao3Yin Chen4Marine School, Ningbo University, 315000 Ningbo, ChinaShandong Institute of Food and Drug Control, Jinan 250000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaA water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa. Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR). The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues. The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→. Most (70%) of the rhamnose was sulfated. UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→. UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro. This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.http://www.mdpi.com/1660-3397/16/8/281green algaeulvanstructureanticoagulant activityimmunoregulation |
| spellingShingle | Peipei Li Songsong Wen Kunlai Sun Yuqin Zhao Yin Chen Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca Marine Drugs green algae ulvan structure anticoagulant activity immunoregulation |
| title | Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca |
| title_full | Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca |
| title_fullStr | Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca |
| title_full_unstemmed | Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca |
| title_short | Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca |
| title_sort | structure and bioactivity screening of a low molecular weight ulvan from the green alga ulothrix flacca |
| topic | green algae ulvan structure anticoagulant activity immunoregulation |
| url | http://www.mdpi.com/1660-3397/16/8/281 |
| work_keys_str_mv | AT peipeili structureandbioactivityscreeningofalowmolecularweightulvanfromthegreenalgaulothrixflacca AT songsongwen structureandbioactivityscreeningofalowmolecularweightulvanfromthegreenalgaulothrixflacca AT kunlaisun structureandbioactivityscreeningofalowmolecularweightulvanfromthegreenalgaulothrixflacca AT yuqinzhao structureandbioactivityscreeningofalowmolecularweightulvanfromthegreenalgaulothrixflacca AT yinchen structureandbioactivityscreeningofalowmolecularweightulvanfromthegreenalgaulothrixflacca |