Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors
Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small RNAs in SP can be used to diagnose men with TGCTs or...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/10/2346 |
| _version_ | 1797534346248192000 |
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| author | Nina Mørup Rytis Stakaitis Ieva Golubickaite Meritxell Riera Marlene Danner Dalgaard Mikkel H. Schierup Niels Jørgensen Gedske Daugaard Anders Juul Kristian Almstrup |
| author_facet | Nina Mørup Rytis Stakaitis Ieva Golubickaite Meritxell Riera Marlene Danner Dalgaard Mikkel H. Schierup Niels Jørgensen Gedske Daugaard Anders Juul Kristian Almstrup |
| author_sort | Nina Mørup |
| collection | DOAJ |
| description | Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small RNAs in SP can be used to diagnose men with TGCTs or the precursor lesions, germ cell neoplasia in situ (GCNIS). Small RNAs isolated from SP from men with TGCTs (<i>n</i> = 18), GCNIS-only (<i>n</i> = 5), and controls (<i>n</i> = 25) were sequenced. SP from men with TGCT/GCNIS (<i>n</i> = 37) and controls (<i>n</i> = 22) were used for validation by RT-qPCR. In general, piRNAs were found at lower levels in SP from men with TGCTs. Ten small RNAs were found at significantly (q-value < 0.05) different levels in SP from men with TGCT/GCNIS than controls. Random forests classification identified sets of small RNAs that could detect either TGCT/GCNIS or GCNIS-only with an area under the curve of 0.98 and 1 in ROC analyses, respectively. RT-qPCR validated hsa-miR-6782-5p to be present at 2.3-fold lower levels (<i>p</i> = 0.02) in the SP from men with TGCTs compared with controls. Small RNAs in SP show potential as novel biomarkers for diagnosing men with TGCT/GCNIS but validation in larger cohorts is needed. |
| first_indexed | 2024-03-10T11:28:20Z |
| format | Article |
| id | doaj.art-fb2f37b52b36486894053288b8d117c8 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T11:28:20Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-fb2f37b52b36486894053288b8d117c82023-11-21T19:28:16ZengMDPI AGCancers2072-66942021-05-011310234610.3390/cancers13102346Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell TumorsNina Mørup0Rytis Stakaitis1Ieva Golubickaite2Meritxell Riera3Marlene Danner Dalgaard4Mikkel H. Schierup5Niels Jørgensen6Gedske Daugaard7Anders Juul8Kristian Almstrup9Department of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkBioinformatics Research Centre, Aarhus University, 8000 Aarhus C, DenmarkDTU Multi-Assay Core, Department of Health Technology, Technical University of Denmark, 2800 Lyngby, DenmarkBioinformatics Research Centre, Aarhus University, 8000 Aarhus C, DenmarkDepartment of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Oncology, Copenhagen University Hospital, 2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, DenmarkCirculating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small RNAs in SP can be used to diagnose men with TGCTs or the precursor lesions, germ cell neoplasia in situ (GCNIS). Small RNAs isolated from SP from men with TGCTs (<i>n</i> = 18), GCNIS-only (<i>n</i> = 5), and controls (<i>n</i> = 25) were sequenced. SP from men with TGCT/GCNIS (<i>n</i> = 37) and controls (<i>n</i> = 22) were used for validation by RT-qPCR. In general, piRNAs were found at lower levels in SP from men with TGCTs. Ten small RNAs were found at significantly (q-value < 0.05) different levels in SP from men with TGCT/GCNIS than controls. Random forests classification identified sets of small RNAs that could detect either TGCT/GCNIS or GCNIS-only with an area under the curve of 0.98 and 1 in ROC analyses, respectively. RT-qPCR validated hsa-miR-6782-5p to be present at 2.3-fold lower levels (<i>p</i> = 0.02) in the SP from men with TGCTs compared with controls. Small RNAs in SP show potential as novel biomarkers for diagnosing men with TGCT/GCNIS but validation in larger cohorts is needed.https://www.mdpi.com/2072-6694/13/10/2346small RNAstesticular cancerdiagnostics |
| spellingShingle | Nina Mørup Rytis Stakaitis Ieva Golubickaite Meritxell Riera Marlene Danner Dalgaard Mikkel H. Schierup Niels Jørgensen Gedske Daugaard Anders Juul Kristian Almstrup Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors Cancers small RNAs testicular cancer diagnostics |
| title | Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors |
| title_full | Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors |
| title_fullStr | Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors |
| title_full_unstemmed | Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors |
| title_short | Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors |
| title_sort | small rnas in seminal plasma as novel biomarkers for germ cell tumors |
| topic | small RNAs testicular cancer diagnostics |
| url | https://www.mdpi.com/2072-6694/13/10/2346 |
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