BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1
<p>Abstract</p> <p>Background</p> <p>Bone morphogenetic proteins (BMPs) belong to the TGF-β superfamily and are secreted proteins with pleiotropic roles in many different cell types. A potential role of BMP-6 in the immune system has been implied by various studies of m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-05-01
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| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/6/9 |
| _version_ | 1818146138137559040 |
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| author | Kersten Christian Sivertsen Einar A Hystad Marit E Forfang Lise Smeland Erlend B Myklebust June H |
| author_facet | Kersten Christian Sivertsen Einar A Hystad Marit E Forfang Lise Smeland Erlend B Myklebust June H |
| author_sort | Kersten Christian |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bone morphogenetic proteins (BMPs) belong to the TGF-β superfamily and are secreted proteins with pleiotropic roles in many different cell types. A potential role of BMP-6 in the immune system has been implied by various studies of malignant and rheumatoid diseases. In the present study, we explored the role of BMP-6 in normal human peripheral blood B cells.</p> <p>Results</p> <p>The B cells were found to express BMP type I and type II receptors and BMP-6 rapidly induced phosphorylation of Smad1/5/8. Furthermore, Smad-phosphorylation was followed by upregulation of <it>Id1 </it>mRNA and Id1 protein, whereas Id2 and Id3 expression was not affected. Furthermore, we found that BMP-6 had an antiproliferative effect both in naïve (CD19<sup>+</sup>CD27<sup>-</sup>) and memory B cells (CD19<sup>+</sup>CD27<sup>+</sup>) stimulated with anti-IgM alone or the combined action of anti-IgM and CD40L. Additionally, BMP-6 induced cell death in activated memory B cells. Importantly, the antiproliferative effect of BMP-6 in B-cells was completely neutralized by the natural antagonist, noggin. Furthermore, B cells were demonstrated to upregulate <it>BMP-6 </it>mRNA upon stimulation with anti-IgM.</p> <p>Conclusion</p> <p>In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad1/5/8 followed by an upregulation of Id1.</p> |
| first_indexed | 2024-12-11T12:14:35Z |
| format | Article |
| id | doaj.art-fb300e0c8595427eb3c0bd5a5f18886a |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-11T12:14:35Z |
| publishDate | 2005-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-fb300e0c8595427eb3c0bd5a5f18886a2022-12-22T01:07:42ZengBMCBMC Immunology1471-21722005-05-0161910.1186/1471-2172-6-9BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1Kersten ChristianSivertsen Einar AHystad Marit EForfang LiseSmeland Erlend BMyklebust June H<p>Abstract</p> <p>Background</p> <p>Bone morphogenetic proteins (BMPs) belong to the TGF-β superfamily and are secreted proteins with pleiotropic roles in many different cell types. A potential role of BMP-6 in the immune system has been implied by various studies of malignant and rheumatoid diseases. In the present study, we explored the role of BMP-6 in normal human peripheral blood B cells.</p> <p>Results</p> <p>The B cells were found to express BMP type I and type II receptors and BMP-6 rapidly induced phosphorylation of Smad1/5/8. Furthermore, Smad-phosphorylation was followed by upregulation of <it>Id1 </it>mRNA and Id1 protein, whereas Id2 and Id3 expression was not affected. Furthermore, we found that BMP-6 had an antiproliferative effect both in naïve (CD19<sup>+</sup>CD27<sup>-</sup>) and memory B cells (CD19<sup>+</sup>CD27<sup>+</sup>) stimulated with anti-IgM alone or the combined action of anti-IgM and CD40L. Additionally, BMP-6 induced cell death in activated memory B cells. Importantly, the antiproliferative effect of BMP-6 in B-cells was completely neutralized by the natural antagonist, noggin. Furthermore, B cells were demonstrated to upregulate <it>BMP-6 </it>mRNA upon stimulation with anti-IgM.</p> <p>Conclusion</p> <p>In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad1/5/8 followed by an upregulation of Id1.</p>http://www.biomedcentral.com/1471-2172/6/9 |
| spellingShingle | Kersten Christian Sivertsen Einar A Hystad Marit E Forfang Lise Smeland Erlend B Myklebust June H BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 BMC Immunology |
| title | BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 |
| title_full | BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 |
| title_fullStr | BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 |
| title_full_unstemmed | BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 |
| title_short | BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1 |
| title_sort | bmp 6 inhibits growth of mature human b cells induction of smad phosphorylation and upregulation of id1 |
| url | http://www.biomedcentral.com/1471-2172/6/9 |
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