Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts
Chinese olive (<i>Canarium album</i> L.), a rich source of polyphenols, can be used as a functional food ingredient. We previously showed that the ethyl acetate fraction of this extract (CO-EtOAc) is an effective anti-inflammatory agent. Therefore, here, we aimed to screen the bioactive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/8/10/441 |
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| author | Yueh-Hsiung Kuo Yu-Te Yeh Sih-Ying Pan Shu-Chen Hsieh |
| author_facet | Yueh-Hsiung Kuo Yu-Te Yeh Sih-Ying Pan Shu-Chen Hsieh |
| author_sort | Yueh-Hsiung Kuo |
| collection | DOAJ |
| description | Chinese olive (<i>Canarium album</i> L.), a rich source of polyphenols, can be used as a functional food ingredient. We previously showed that the ethyl acetate fraction of this extract (CO-EtOAc) is an effective anti-inflammatory agent. Therefore, here, we aimed to screen the bioactive fractions extracted from CO-EtOAc using different isolation techniques, and purify the bioactive compounds based on their cytotoxic and anti-inflammatory abilities. CO-EtOAc was fractionated using silica gel and Sephadex column chromatography, and the active compounds were isolated and purified by high-performance liquid chromatography (HPLC). The structures of the resulting compounds were identified using proton nuclear magnetic resonance (NMR) spectra. Activity-directed fractionation and purification were used to identify the following active compounds with anti-inflammatory effects using lipopolysaccharide (LPS)-stimulated mouse macrophages: sitoindoside I, amentoflavone, tetrahydroamentoflavone and protocatechuic acid. For the first time, sitoindoside I and tetrahydroamentoflavone were isolated from Chinese olive, and the anti-inflammatory compounds of CO-EtOAc were identified, suggesting its potential for used as a health food ingredient. |
| first_indexed | 2024-12-11T21:29:03Z |
| format | Article |
| id | doaj.art-fb329808d77c44bd820c7bfd425d6e75 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-11T21:29:03Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fb329808d77c44bd820c7bfd425d6e752022-12-22T00:50:14ZengMDPI AGFoods2304-81582019-09-0181044110.3390/foods8100441foods8100441Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit ExtractsYueh-Hsiung Kuo0Yu-Te Yeh1Sih-Ying Pan2Shu-Chen Hsieh3Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, TaiwanDepartments of Medicine and Biological Chemistry, Johns Hopkins University School of Medicine and Johns Hopkins All Children’s Hospital, St. Petersburg, FL 33701, USAInstitute of Food Science and Technology, National Taiwan University; Taipei 106, TaiwanInstitute of Food Science and Technology, National Taiwan University; Taipei 106, TaiwanChinese olive (<i>Canarium album</i> L.), a rich source of polyphenols, can be used as a functional food ingredient. We previously showed that the ethyl acetate fraction of this extract (CO-EtOAc) is an effective anti-inflammatory agent. Therefore, here, we aimed to screen the bioactive fractions extracted from CO-EtOAc using different isolation techniques, and purify the bioactive compounds based on their cytotoxic and anti-inflammatory abilities. CO-EtOAc was fractionated using silica gel and Sephadex column chromatography, and the active compounds were isolated and purified by high-performance liquid chromatography (HPLC). The structures of the resulting compounds were identified using proton nuclear magnetic resonance (NMR) spectra. Activity-directed fractionation and purification were used to identify the following active compounds with anti-inflammatory effects using lipopolysaccharide (LPS)-stimulated mouse macrophages: sitoindoside I, amentoflavone, tetrahydroamentoflavone and protocatechuic acid. For the first time, sitoindoside I and tetrahydroamentoflavone were isolated from Chinese olive, and the anti-inflammatory compounds of CO-EtOAc were identified, suggesting its potential for used as a health food ingredient.https://www.mdpi.com/2304-8158/8/10/441amentoflavonechinese olive (<i>canarium album</i> l.)protocatechuic acidsitoindoside itetrahydroamentoflavone |
| spellingShingle | Yueh-Hsiung Kuo Yu-Te Yeh Sih-Ying Pan Shu-Chen Hsieh Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts Foods amentoflavone chinese olive (<i>canarium album</i> l.) protocatechuic acid sitoindoside i tetrahydroamentoflavone |
| title | Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts |
| title_full | Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts |
| title_fullStr | Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts |
| title_full_unstemmed | Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts |
| title_short | Identification and Structural Elucidation of Anti-Inflammatory Compounds from Chinese Olive (<i>Canarium Album</i> L.) Fruit Extracts |
| title_sort | identification and structural elucidation of anti inflammatory compounds from chinese olive i canarium album i l fruit extracts |
| topic | amentoflavone chinese olive (<i>canarium album</i> l.) protocatechuic acid sitoindoside i tetrahydroamentoflavone |
| url | https://www.mdpi.com/2304-8158/8/10/441 |
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