Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.)
Olive (Olea europaea L.) is an ancient tree species in the Mediterranean, but the lack of knowledge about aluminum-resistant varieties limits its introduction to acidic soil. The objective of this study was to have a comprehensive evaluation of the response to aluminum stress in olive tree at germpl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.968499/full |
| _version_ | 1811312582165790720 |
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| author | Erli Niu Song Gao Xiaomin Yu Ali Soleimani Shenlong Zhu |
| author_facet | Erli Niu Song Gao Xiaomin Yu Ali Soleimani Shenlong Zhu |
| author_sort | Erli Niu |
| collection | DOAJ |
| description | Olive (Olea europaea L.) is an ancient tree species in the Mediterranean, but the lack of knowledge about aluminum-resistant varieties limits its introduction to acidic soil. The objective of this study was to have a comprehensive evaluation of the response to aluminum stress in olive tree at germplasm, metabolome, and transcriptome levels. In this experiment, seedlings of 97 olive germplasm with 1.0–3.0 cm roots and two leaves were treated with 50 μM Al3+ (pH = 5.0). By factor analysis of the traits of defoliation rate, rooting rate, length of extended root, and length of new root, 97 germplasm were classified into five different groups according to their diverse responses to aluminum stress: 5 highly resistant (5.15%), 30 moderately resistant (30.93%), 31 general (31.96%), 23 moderately sensitive (23.71%), and 8 highly sensitive (8.25%) germplasm. The three most sensitive and three most resistant germplasm were further used for metabolome and transcriptome analysis. Exposed to aluminum stress, 96 differentially accumulated metabolites (DAMs)/4,845 differentially expressed genes (DEGs) and 66 DAMs/2,752 DEGs were identified in highly sensitive and resistant germplasm, respectively. Using multi-omics technology, the pathways and related DAMs/DEGs involved in cell wall/cytoplasm receptors, reactive oxygen species balance, hormone induction, synthesis of organic acids, Al3+ transport, and synthesis of metabolites were identified to mainly regulate the response to aluminum stress in olive. This study provides a theoretical guide and prior germplasm and genes for further genetic improvement of aluminum tolerance in the olive tree. |
| first_indexed | 2024-04-13T10:39:50Z |
| format | Article |
| id | doaj.art-fb3528060fc34614a4ff9025013819f1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T10:39:50Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-fb3528060fc34614a4ff9025013819f12022-12-22T02:49:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.968499968499Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.)Erli Niu0Song Gao1Xiaomin Yu2Ali Soleimani3Shenlong Zhu4Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Digital Dry Land Crops of Zhejiang Province, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Digital Dry Land Crops of Zhejiang Province, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaFaculty of Agriculture, University of Zanjan, Zanjan, IranKey Laboratory of Digital Dry Land Crops of Zhejiang Province, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaOlive (Olea europaea L.) is an ancient tree species in the Mediterranean, but the lack of knowledge about aluminum-resistant varieties limits its introduction to acidic soil. The objective of this study was to have a comprehensive evaluation of the response to aluminum stress in olive tree at germplasm, metabolome, and transcriptome levels. In this experiment, seedlings of 97 olive germplasm with 1.0–3.0 cm roots and two leaves were treated with 50 μM Al3+ (pH = 5.0). By factor analysis of the traits of defoliation rate, rooting rate, length of extended root, and length of new root, 97 germplasm were classified into five different groups according to their diverse responses to aluminum stress: 5 highly resistant (5.15%), 30 moderately resistant (30.93%), 31 general (31.96%), 23 moderately sensitive (23.71%), and 8 highly sensitive (8.25%) germplasm. The three most sensitive and three most resistant germplasm were further used for metabolome and transcriptome analysis. Exposed to aluminum stress, 96 differentially accumulated metabolites (DAMs)/4,845 differentially expressed genes (DEGs) and 66 DAMs/2,752 DEGs were identified in highly sensitive and resistant germplasm, respectively. Using multi-omics technology, the pathways and related DAMs/DEGs involved in cell wall/cytoplasm receptors, reactive oxygen species balance, hormone induction, synthesis of organic acids, Al3+ transport, and synthesis of metabolites were identified to mainly regulate the response to aluminum stress in olive. This study provides a theoretical guide and prior germplasm and genes for further genetic improvement of aluminum tolerance in the olive tree.https://www.frontiersin.org/articles/10.3389/fpls.2022.968499/fullolive (Olea europaea L.)aluminum tolerancegermplasm evaluationmetabolometranscriptome |
| spellingShingle | Erli Niu Song Gao Xiaomin Yu Ali Soleimani Shenlong Zhu Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) Frontiers in Plant Science olive (Olea europaea L.) aluminum tolerance germplasm evaluation metabolome transcriptome |
| title | Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) |
| title_full | Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) |
| title_fullStr | Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) |
| title_full_unstemmed | Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) |
| title_short | Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.) |
| title_sort | comprehensive evaluation of the response to aluminum stress in olive tree olea europaea l |
| topic | olive (Olea europaea L.) aluminum tolerance germplasm evaluation metabolome transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.968499/full |
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