An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy
In the present study, an efficient in vivo drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the in vitro drug-induced cell apoptosis. Further...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.839078/full |
| _version_ | 1811204763267629056 |
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| author | Fuqiang Xing Fuqiang Xing Fuqiang Xing Nana Ai Shigao Huang Cheng Jiang Muhammad Jameel Mughal Wei Ge Guanyu Wang Guanyu Wang Chu-Xia Deng |
| author_facet | Fuqiang Xing Fuqiang Xing Fuqiang Xing Nana Ai Shigao Huang Cheng Jiang Muhammad Jameel Mughal Wei Ge Guanyu Wang Guanyu Wang Chu-Xia Deng |
| author_sort | Fuqiang Xing |
| collection | DOAJ |
| description | In the present study, an efficient in vivo drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the in vitro drug-induced cell apoptosis. Furthermore, the C3 expressing cancer cells were then injected into zebrafish embryos and nude mice to establish the corresponding in vivo xenograft models. We found that cancer cell lines expressing C3 were effective in detecting cell death following drug treatment, including the detection of the tipping point of apoptosis. The drug-induced cell apoptosis was also observed in both zebrafish embryos and nude mice xenograft models. Overall, the FRET-based platform, through in vivo imaging, is potentially useful to improve drug screening efficiency. |
| first_indexed | 2024-04-12T03:19:13Z |
| format | Article |
| id | doaj.art-fb362d8ae44d42c0b954b63d8fb2352f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T03:19:13Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-fb362d8ae44d42c0b954b63d8fb2352f2022-12-22T03:49:58ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-02-011010.3389/fbioe.2022.839078839078An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer TherapyFuqiang Xing0Fuqiang Xing1Fuqiang Xing2Nana Ai3Shigao Huang4Cheng Jiang5Muhammad Jameel Mughal6Wei Ge7Guanyu Wang8Guanyu Wang9Chu-Xia Deng10Cancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaDepartment of Biology, School of Life Sciences, Guangdong Provincial Key Laboratory of Computational Science and Material Design, Southern University of Science and Technology, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Biomedical Imaging and Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, ChinaCentre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaCancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaSchool of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, ChinaCancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaCentre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaDepartment of Biology, School of Life Sciences, Guangdong Provincial Key Laboratory of Computational Science and Material Design, Southern University of Science and Technology, Shenzhen, ChinaSchool of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, ChinaCancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, ChinaIn the present study, an efficient in vivo drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the in vitro drug-induced cell apoptosis. Furthermore, the C3 expressing cancer cells were then injected into zebrafish embryos and nude mice to establish the corresponding in vivo xenograft models. We found that cancer cell lines expressing C3 were effective in detecting cell death following drug treatment, including the detection of the tipping point of apoptosis. The drug-induced cell apoptosis was also observed in both zebrafish embryos and nude mice xenograft models. Overall, the FRET-based platform, through in vivo imaging, is potentially useful to improve drug screening efficiency.https://www.frontiersin.org/articles/10.3389/fbioe.2022.839078/fullapoptosiscancer therapydrug discoveryFRET techniquexenograft model |
| spellingShingle | Fuqiang Xing Fuqiang Xing Fuqiang Xing Nana Ai Shigao Huang Cheng Jiang Muhammad Jameel Mughal Wei Ge Guanyu Wang Guanyu Wang Chu-Xia Deng An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy Frontiers in Bioengineering and Biotechnology apoptosis cancer therapy drug discovery FRET technique xenograft model |
| title | An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy |
| title_full | An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy |
| title_fullStr | An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy |
| title_full_unstemmed | An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy |
| title_short | An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy |
| title_sort | in vivo fluorescence resonance energy transfer based imaging platform for targeted drug discovery and cancer therapy |
| topic | apoptosis cancer therapy drug discovery FRET technique xenograft model |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.839078/full |
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