The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate
A laboratory incubation experiment was conducted to examine the effects of 3,4-dimethylpyrazole phosphate (DMPP) on the transformation of urea-N and associated microbial communities in a low-fertility brown soil. The soil was supplied with urea at 180 kg N/ha and DMPP at 0, 1.8 or 18 kg/ha, while so...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2013-09-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-201309-0003_the-response-of-nitrifier-n-fixer-and-denitrifier-gene-copy-numbers-to-the-nitrification-inhibitor-3-4-dimethy.php |
| _version_ | 1828007431060324352 |
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| author | X.X. Dong L.L. Zhang Z.J. Wu H.W. Zhang P. Gong |
| author_facet | X.X. Dong L.L. Zhang Z.J. Wu H.W. Zhang P. Gong |
| author_sort | X.X. Dong |
| collection | DOAJ |
| description | A laboratory incubation experiment was conducted to examine the effects of 3,4-dimethylpyrazole phosphate (DMPP) on the transformation of urea-N and associated microbial communities in a low-fertility brown soil. The soil was supplied with urea at 180 kg N/ha and DMPP at 0, 1.8 or 18 kg/ha, while soil that was not supplied with urea and DMPP was treated as the control. Soil mineral nitrogen (NH4+-N and NO3--N) was measured at regular intervals, and temporal variations in the population sizes of nitrogen-cycling microbes were determined using real-time PCR. Compared to the urea-alone treatment, the additional application of DMPP maintained significantly higher (P < 0.05) NH4+-N concentrations and lower NO3--N (P < 0.05) concentrations in the soil and decreased the population sizes of ammonia oxidizing and denitrifying microbes but promoted the growth of nitrogen-fixing bacteria. The results suggest that the application of DMPP is beneficial in improving the availability of fertilizer-N for plant uptake. This study is the first comprehensive investigation to examine the effects of DMPP on the functional microorganisms that are important in nitrogen-cycling processes. |
| first_indexed | 2024-04-10T08:11:27Z |
| format | Article |
| id | doaj.art-fb36369c90a54941a215d38d17c8022a |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:11:27Z |
| publishDate | 2013-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-fb36369c90a54941a215d38d17c8022a2023-02-23T03:46:00ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682013-09-0159939840310.17221/165/2013-PSEpse-201309-0003The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphateX.X. Dong0L.L. Zhang1Z.J. Wu2H.W. Zhang3P. Gong4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. ChinaInstitute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. ChinaInstitute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. ChinaInstitute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. ChinaInstitute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. ChinaA laboratory incubation experiment was conducted to examine the effects of 3,4-dimethylpyrazole phosphate (DMPP) on the transformation of urea-N and associated microbial communities in a low-fertility brown soil. The soil was supplied with urea at 180 kg N/ha and DMPP at 0, 1.8 or 18 kg/ha, while soil that was not supplied with urea and DMPP was treated as the control. Soil mineral nitrogen (NH4+-N and NO3--N) was measured at regular intervals, and temporal variations in the population sizes of nitrogen-cycling microbes were determined using real-time PCR. Compared to the urea-alone treatment, the additional application of DMPP maintained significantly higher (P < 0.05) NH4+-N concentrations and lower NO3--N (P < 0.05) concentrations in the soil and decreased the population sizes of ammonia oxidizing and denitrifying microbes but promoted the growth of nitrogen-fixing bacteria. The results suggest that the application of DMPP is beneficial in improving the availability of fertilizer-N for plant uptake. This study is the first comprehensive investigation to examine the effects of DMPP on the functional microorganisms that are important in nitrogen-cycling processes.https://pse.agriculturejournals.cz/artkey/pse-201309-0003_the-response-of-nitrifier-n-fixer-and-denitrifier-gene-copy-numbers-to-the-nitrification-inhibitor-3-4-dimethy.phpammonia oxidizing bacteriaammonia oxidizing archaeanitrogen cyclingrt-pcr |
| spellingShingle | X.X. Dong L.L. Zhang Z.J. Wu H.W. Zhang P. Gong The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate Plant, Soil and Environment ammonia oxidizing bacteria ammonia oxidizing archaea nitrogen cycling rt-pcr |
| title | The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate |
| title_full | The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate |
| title_fullStr | The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate |
| title_full_unstemmed | The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate |
| title_short | The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate |
| title_sort | response of nitrifier n fixer and denitrifier gene copy numbers to the nitrification inhibitor 3 4 dimethylpyrazole phosphate |
| topic | ammonia oxidizing bacteria ammonia oxidizing archaea nitrogen cycling rt-pcr |
| url | https://pse.agriculturejournals.cz/artkey/pse-201309-0003_the-response-of-nitrifier-n-fixer-and-denitrifier-gene-copy-numbers-to-the-nitrification-inhibitor-3-4-dimethy.php |
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