Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
ABSTRACT Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was obs...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2018-06-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100452&lng=en&tlng=en |
| _version_ | 1819067815804534784 |
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| author | Amina Elahi Abdul Rehman |
| author_facet | Amina Elahi Abdul Rehman |
| author_sort | Amina Elahi |
| collection | DOAJ |
| description | ABSTRACT Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 ± 3.19) and GSSG (3.7 ± 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 ± 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 ± 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50°C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30°C (pH 7). Activity of both ChRs was enhanced in the presence of Mg, Na, Co and Ca but strongly inhibited by Hg cations. Cr(VI) uptake capabilities were ranged between 43-97% in R. mucilaginosa and 35-88% in T. asahii. One dimensional electrophoresis revealed enriched bands of cysteine rich metallothioneins suggesting some differential proteins could be overexpressed under Cr(VI) stress. |
| first_indexed | 2024-12-21T16:24:15Z |
| format | Article |
| id | doaj.art-fb3b2dd51ffb4789b0cf44b7efbbf20b |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T16:24:15Z |
| publishDate | 2018-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-fb3b2dd51ffb4789b0cf44b7efbbf20b2022-12-21T18:57:30ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242018-06-0160010.1590/1678-4324-2017160394S1516-89132017000100452Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial WastewaterAmina ElahiAbdul RehmanABSTRACT Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 ± 3.19) and GSSG (3.7 ± 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 ± 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 ± 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50°C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30°C (pH 7). Activity of both ChRs was enhanced in the presence of Mg, Na, Co and Ca but strongly inhibited by Hg cations. Cr(VI) uptake capabilities were ranged between 43-97% in R. mucilaginosa and 35-88% in T. asahii. One dimensional electrophoresis revealed enriched bands of cysteine rich metallothioneins suggesting some differential proteins could be overexpressed under Cr(VI) stress.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100452&lng=en&tlng=enTrichosporon asahiiRhodotorula mucilaginosawastewaterresistancebioaccumulation |
| spellingShingle | Amina Elahi Abdul Rehman Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater Brazilian Archives of Biology and Technology Trichosporon asahii Rhodotorula mucilaginosa wastewater resistance bioaccumulation |
| title | Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater |
| title_full | Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater |
| title_fullStr | Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater |
| title_full_unstemmed | Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater |
| title_short | Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater |
| title_sort | oxidative stress chromium resistance and uptake by fungi isolated from industrial wastewater |
| topic | Trichosporon asahii Rhodotorula mucilaginosa wastewater resistance bioaccumulation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100452&lng=en&tlng=en |
| work_keys_str_mv | AT aminaelahi oxidativestresschromiumresistanceanduptakebyfungiisolatedfromindustrialwastewater AT abdulrehman oxidativestresschromiumresistanceanduptakebyfungiisolatedfromindustrialwastewater |