Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria
Abstract Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environ...
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| Format: | Article |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46684-z |
| _version_ | 1827301090042839040 |
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| author | Maria Nieto-Rosado Kirsty Sands Edward A. R. Portal Kathryn M. Thomson Maria J. Carvalho Jordan Mathias Rebecca Milton Calie Dyer Chinenye Akpulu Ian Boostrom Patrick Hogan Habiba Saif Ana D. Sanches Ferreira Thomas Hender Barbra Portal Robert Andrews W. John Watkins Rabaab Zahra Haider Shirazi Adil Muhammad Syed Najeeb Ullah Muhammad Hilal Jan Shermeen Akif Kenneth C. Iregbu Fatima Modibbo Stella Uwaezuoke Lamidi Audu Chinago P. Edwin Ashiru H. Yusuf Adeola Adeleye Aisha S. Mukkadas Jean Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andrew N. H. Bulabula Andrew Whitelaw Lauren Roberts Grace Chan Delayehu Bekele Semaria Solomon Mahlet Abayneh Gesit Metaferia Group BARNARDS Timothy R. Walsh |
| author_facet | Maria Nieto-Rosado Kirsty Sands Edward A. R. Portal Kathryn M. Thomson Maria J. Carvalho Jordan Mathias Rebecca Milton Calie Dyer Chinenye Akpulu Ian Boostrom Patrick Hogan Habiba Saif Ana D. Sanches Ferreira Thomas Hender Barbra Portal Robert Andrews W. John Watkins Rabaab Zahra Haider Shirazi Adil Muhammad Syed Najeeb Ullah Muhammad Hilal Jan Shermeen Akif Kenneth C. Iregbu Fatima Modibbo Stella Uwaezuoke Lamidi Audu Chinago P. Edwin Ashiru H. Yusuf Adeola Adeleye Aisha S. Mukkadas Jean Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andrew N. H. Bulabula Andrew Whitelaw Lauren Roberts Grace Chan Delayehu Bekele Semaria Solomon Mahlet Abayneh Gesit Metaferia Group BARNARDS Timothy R. Walsh |
| author_sort | Maria Nieto-Rosado |
| collection | DOAJ |
| description | Abstract Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (bla CTX-M-15) and carbapenemases (bla NDM, bla OXA-48-like and bla KPC), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness to strains causing neonatal sepsis. Higher prevalence of carbapenemases is observed in Pakistan, Bangladesh, and Ethiopia, compared to other countries, and are mostly found in surfaces near the sink drain. Klebsiella pneumoniae, Enterobacter hormaechei, Acinetobacter baumannii, Serratia marcescens and Leclercia adecarboxylata are dominant; ST15 K. pneumoniae is identified from the same ward on multiple occasions suggesting clonal persistence within the same environment, and is found to be identical to isolates causing neonatal sepsis in Pakistan over similar time periods. Our data suggests persistence of dominant clones across multiple time points, highlighting the need for assessment of Infection Prevention and Control guidelines. |
| first_indexed | 2024-04-24T16:17:09Z |
| format | Article |
| id | doaj.art-fb3f6d5c019a46c0ac7a6466db812ad7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T16:17:09Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fb3f6d5c019a46c0ac7a6466db812ad72024-03-31T11:26:01ZengNature PortfolioNature Communications2041-17232024-03-0115111210.1038/s41467-024-46684-zColonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteriaMaria Nieto-Rosado0Kirsty Sands1Edward A. R. Portal2Kathryn M. Thomson3Maria J. Carvalho4Jordan Mathias5Rebecca Milton6Calie Dyer7Chinenye Akpulu8Ian Boostrom9Patrick Hogan10Habiba Saif11Ana D. Sanches Ferreira12Thomas Hender13Barbra Portal14Robert Andrews15W. John Watkins16Rabaab Zahra17Haider Shirazi18Adil Muhammad19Syed Najeeb Ullah20Muhammad Hilal Jan21Shermeen Akif22Kenneth C. Iregbu23Fatima Modibbo24Stella Uwaezuoke25Lamidi Audu26Chinago P. Edwin27Ashiru H. Yusuf28Adeola Adeleye29Aisha S. Mukkadas30Jean Baptiste Mazarati31Aniceth Rucogoza32Lucie Gaju33Shaheen Mehtar34Andrew N. H. Bulabula35Andrew Whitelaw36Lauren Roberts37Grace Chan38Delayehu Bekele39Semaria Solomon40Mahlet Abayneh41Gesit Metaferia42Group BARNARDSTimothy R. Walsh43Department of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordDepartment of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordDepartment of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordDepartment of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDepartment of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityDepartment of Microbiology, Quaid-i-Azam UniversityPakistan Institute of Medical SciencesDepartment of Microbiology, Quaid-i-Azam UniversityDepartment of Microbiology, Quaid-i-Azam UniversityDepartment of Microbiology, Quaid-i-Azam UniversityDepartment of Microbiology, Quaid-i-Azam UniversityNational Hospital AbujaDébbo Africa, LekkiFederal Medical Centre JabiNational Hospital AbujaDepartment of Microbiology, Medway Maritime Hospital NHS Foundation TrustAminu Kano Teaching HospitalMurtala Muhammad Specialist HospitalMurtala Muhammad Specialist HospitalThe National Reference Laboratory, Rwanda Biomedical CentreThe National Reference Laboratory, Rwanda Biomedical CentreThe National Reference Laboratory, Rwanda Biomedical CentreUnit of IPC, Stellenbosch UniversityInfection Control Africa NetworkDivision of Medical Microbiology, Stellenbosch UniversityDivision of Medical Microbiology, Stellenbosch UniversityDepartment of Pediatrics, Boston Children’s Hospital, Harvard Medical SchoolDepartment of Epidemiology, Harvard T.H. Chan School of Public HealthDepartment of Microbiology, Immunology and Parasitology, St Paul’s Hospital Millennium Medical CollegeDepartment of Pediatrics and Child Health, St Paul’s Hospital Millennium Medical CollegeDepartment of Microbiology, Immunology and Parasitology, St Paul’s Hospital Millennium Medical CollegeDepartment of Biology, Ineos Oxford Institute for Antimicrobial Research, University of OxfordAbstract Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (bla CTX-M-15) and carbapenemases (bla NDM, bla OXA-48-like and bla KPC), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness to strains causing neonatal sepsis. Higher prevalence of carbapenemases is observed in Pakistan, Bangladesh, and Ethiopia, compared to other countries, and are mostly found in surfaces near the sink drain. Klebsiella pneumoniae, Enterobacter hormaechei, Acinetobacter baumannii, Serratia marcescens and Leclercia adecarboxylata are dominant; ST15 K. pneumoniae is identified from the same ward on multiple occasions suggesting clonal persistence within the same environment, and is found to be identical to isolates causing neonatal sepsis in Pakistan over similar time periods. Our data suggests persistence of dominant clones across multiple time points, highlighting the need for assessment of Infection Prevention and Control guidelines.https://doi.org/10.1038/s41467-024-46684-z |
| spellingShingle | Maria Nieto-Rosado Kirsty Sands Edward A. R. Portal Kathryn M. Thomson Maria J. Carvalho Jordan Mathias Rebecca Milton Calie Dyer Chinenye Akpulu Ian Boostrom Patrick Hogan Habiba Saif Ana D. Sanches Ferreira Thomas Hender Barbra Portal Robert Andrews W. John Watkins Rabaab Zahra Haider Shirazi Adil Muhammad Syed Najeeb Ullah Muhammad Hilal Jan Shermeen Akif Kenneth C. Iregbu Fatima Modibbo Stella Uwaezuoke Lamidi Audu Chinago P. Edwin Ashiru H. Yusuf Adeola Adeleye Aisha S. Mukkadas Jean Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andrew N. H. Bulabula Andrew Whitelaw Lauren Roberts Grace Chan Delayehu Bekele Semaria Solomon Mahlet Abayneh Gesit Metaferia Group BARNARDS Timothy R. Walsh Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria Nature Communications |
| title | Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria |
| title_full | Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria |
| title_fullStr | Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria |
| title_full_unstemmed | Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria |
| title_short | Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria |
| title_sort | colonisation of hospital surfaces from low and middle income countries by extended spectrum β lactamase and carbapenemase producing bacteria |
| url | https://doi.org/10.1038/s41467-024-46684-z |
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