Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice
Grain yield is a polygenic trait that can be influenced by environmental factors and genetic compositions at all plant growth stages. Currently, the molecular mechanisms behind the coordination of the interaction between grain yield-related traits remain unknown. In this study, we characterized the...
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| Format: | Article |
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Elsevier
2022-05-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630822000245 |
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| author | Kai Liu Minjuan Li Bin Zhang Xuming Yin Xinjie Xia Manling Wang Yanchun Cui |
| author_facet | Kai Liu Minjuan Li Bin Zhang Xuming Yin Xinjie Xia Manling Wang Yanchun Cui |
| author_sort | Kai Liu |
| collection | DOAJ |
| description | Grain yield is a polygenic trait that can be influenced by environmental factors and genetic compositions at all plant growth stages. Currently, the molecular mechanisms behind the coordination of the interaction between grain yield-related traits remain unknown. In this study, we characterized the function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes that are transcribed into DUF1645 domain-containing proteins in relation to the grain length, grain weight, and drought stress-tolerance of rice. The transgenic plants with overexpressing or heterologous high levels of Poaceae OsSGL ortholog genes exhibited longer grain size than the wild type plants. Larger cells were seen in panicles of the four transgenic lines with paraffin sectioning and scanning electron microscopy analyses. In addition, four Poaceae OsSGL ortholog genes positively affected the drought tolerance of rice. Four transgenic plants displayed higher resistance to drought stress at the seedling and vegetative stages. RNA-sequencing and qRT-PCR results indicated that over- or heterologous-expression of four Poaceae OsSGL ortholog genes also affected the transcriptome of rice plants. These genes may play a role in auxin and cytokinin biosynthesis and their transduction pathways. Taken together, these results suggested that the four OsSGL orthologs have a conserved function in the regulation of stress-tolerance and cell growth by modulating hormonal biosynthesis and signaling. |
| first_indexed | 2024-04-13T23:25:44Z |
| format | Article |
| id | doaj.art-fb40606054a54c3792674ad267fa9f67 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-04-13T23:25:44Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
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| series | Rice Science |
| spelling | doaj.art-fb40606054a54c3792674ad267fa9f672022-12-22T02:25:04ZengElsevierRice Science1672-63082022-05-01293257267Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in RiceKai Liu0Minjuan Li1Bin Zhang2Xuming Yin3Xinjie Xia4Manling Wang5Yanchun Cui6Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; University of the Chinese Academy of Sciences, Beijing 100049, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaCollege of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Corresponding author:Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Corresponding author:Grain yield is a polygenic trait that can be influenced by environmental factors and genetic compositions at all plant growth stages. Currently, the molecular mechanisms behind the coordination of the interaction between grain yield-related traits remain unknown. In this study, we characterized the function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes that are transcribed into DUF1645 domain-containing proteins in relation to the grain length, grain weight, and drought stress-tolerance of rice. The transgenic plants with overexpressing or heterologous high levels of Poaceae OsSGL ortholog genes exhibited longer grain size than the wild type plants. Larger cells were seen in panicles of the four transgenic lines with paraffin sectioning and scanning electron microscopy analyses. In addition, four Poaceae OsSGL ortholog genes positively affected the drought tolerance of rice. Four transgenic plants displayed higher resistance to drought stress at the seedling and vegetative stages. RNA-sequencing and qRT-PCR results indicated that over- or heterologous-expression of four Poaceae OsSGL ortholog genes also affected the transcriptome of rice plants. These genes may play a role in auxin and cytokinin biosynthesis and their transduction pathways. Taken together, these results suggested that the four OsSGL orthologs have a conserved function in the regulation of stress-tolerance and cell growth by modulating hormonal biosynthesis and signaling.http://www.sciencedirect.com/science/article/pii/S1672630822000245graminaceous cereal cropOsSGL genedrought resistancegrain lengthgrain weight |
| spellingShingle | Kai Liu Minjuan Li Bin Zhang Xuming Yin Xinjie Xia Manling Wang Yanchun Cui Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice Rice Science graminaceous cereal crop OsSGL gene drought resistance grain length grain weight |
| title | Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice |
| title_full | Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice |
| title_fullStr | Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice |
| title_full_unstemmed | Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice |
| title_short | Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice |
| title_sort | poaceae orthologs of rice ossgl duf1645 domain containing genes positively regulate drought tolerance grain length and weight in rice |
| topic | graminaceous cereal crop OsSGL gene drought resistance grain length grain weight |
| url | http://www.sciencedirect.com/science/article/pii/S1672630822000245 |
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