Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis
Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together wit...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2017-07-01
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| Series: | Research Journal of Life Science |
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| Online Access: | http://rjls.ub.ac.id/index.php/rjls/article/view/145 |
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| author | Hani Susianti Wisnu Barlianto Dian Sukma Hanggara Kusworini Handono Purwanto Adipireno Lisyani Suromo |
| author_facet | Hani Susianti Wisnu Barlianto Dian Sukma Hanggara Kusworini Handono Purwanto Adipireno Lisyani Suromo |
| author_sort | Hani Susianti |
| collection | DOAJ |
| description | Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.
Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.
Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.
Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN. |
| first_indexed | 2024-12-21T16:09:40Z |
| format | Article |
| id | doaj.art-fb41402617554cabac3e570f5118ae5c |
| institution | Directory Open Access Journal |
| issn | 2355-9926 |
| language | English |
| last_indexed | 2024-12-21T16:09:40Z |
| publishDate | 2017-07-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Research Journal of Life Science |
| spelling | doaj.art-fb41402617554cabac3e570f5118ae5c2022-12-21T18:57:50ZengUniversity of BrawijayaResearch Journal of Life Science2355-99262017-07-0141414910.21776/ub.rjls.2017.004.01.677Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus NephritisHani Susianti0Wisnu Barlianto1Dian Sukma Hanggara2Kusworini Handono3Purwanto Adipireno4Lisyani SuromoDepartment of Clinical Pathology, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, East Java, IndonesiaDepartment of Pediatrics, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital MalangDepartment of Clinical Pathology, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, East Java, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, East Java, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Diponegoro University/Dr. Kariadi General Hospital SemarangBackground. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity. Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA. Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively. Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN.http://rjls.ub.ac.id/index.php/rjls/article/view/145Lupus nephritis activityPatterns of urine specific proteinsUrine alpha-1 antitrypsin |
| spellingShingle | Hani Susianti Wisnu Barlianto Dian Sukma Hanggara Kusworini Handono Purwanto Adipireno Lisyani Suromo Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Research Journal of Life Science Lupus nephritis activity Patterns of urine specific proteins Urine alpha-1 antitrypsin |
| title | Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis |
| title_full | Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis |
| title_fullStr | Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis |
| title_full_unstemmed | Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis |
| title_short | Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis |
| title_sort | urine specific proteins and alpha 1 antitrypsin concentrations to assess the severity of lupus nephritis |
| topic | Lupus nephritis activity Patterns of urine specific proteins Urine alpha-1 antitrypsin |
| url | http://rjls.ub.ac.id/index.php/rjls/article/view/145 |
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