Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation
Melatonin is a promising reagent that can improve assisted reproductive technology (ART) outcomes in infertility patients. However, melatonin is not effective for all infertile patients, and it remains unclear for which patients melatonin would be effective. This study examined the effects of melato...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6731 |
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| author | Isao Tamura Hiroshi Tamura Mai Kawamoto-Jozaki Yuichiro Shirafuta Taishi Fujimura Yumiko Doi-Tanaka Yumiko Mihara Toshiaki Taketani Norihiro Sugino |
| author_facet | Isao Tamura Hiroshi Tamura Mai Kawamoto-Jozaki Yuichiro Shirafuta Taishi Fujimura Yumiko Doi-Tanaka Yumiko Mihara Toshiaki Taketani Norihiro Sugino |
| author_sort | Isao Tamura |
| collection | DOAJ |
| description | Melatonin is a promising reagent that can improve assisted reproductive technology (ART) outcomes in infertility patients. However, melatonin is not effective for all infertile patients, and it remains unclear for which patients melatonin would be effective. This study examined the effects of melatonin on ART outcomes and examined its mechanisms. Melatonin increased the fertilization rate in patients whose fertilization rates in the previous cycle were less than 50%, but not in patients whose fertilization rates were more than 50% in the previous cycle. Melatonin increased the blastocyst formation rate in patients whose embryo development rates in the previous cycle were less than 50%, but not in patients whose embryo development rates were more than 50% in the previous cycle. To clarify its mechanisms, transcriptome changes by melatonin treatment in granulosa cells (GCs) of the patients were examined by RNA-sequence. Melatonin treatment altered the transcriptomes of GCs of patients with poor ART outcomes so that they were similar to the transcriptomes of patients with good ART outcomes. The altered genes were associated with the inhibition of cell death and T-cell activity, and the activation of steroidogenesis and angiogenesis. Melatonin treatment was effective for patients with poor fertilization rates and poor embryo development rates in the previous ART cycle. Melatonin alters the GCs transcriptome and, thus, their functions, and this could improve the oocyte quality, leading to good ART outcomes. |
| first_indexed | 2024-03-09T23:33:41Z |
| format | Article |
| id | doaj.art-fb41dba95965485ea8feeaf1520f2b75 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:33:41Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fb41dba95965485ea8feeaf1520f2b752023-11-23T17:05:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312673110.3390/ijms23126731Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst FormationIsao Tamura0Hiroshi Tamura1Mai Kawamoto-Jozaki2Yuichiro Shirafuta3Taishi Fujimura4Yumiko Doi-Tanaka5Yumiko Mihara6Toshiaki Taketani7Norihiro Sugino8Department of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Yamaguchi University, Minamikogushi 1-1-1, Ube 755-8505, JapanMelatonin is a promising reagent that can improve assisted reproductive technology (ART) outcomes in infertility patients. However, melatonin is not effective for all infertile patients, and it remains unclear for which patients melatonin would be effective. This study examined the effects of melatonin on ART outcomes and examined its mechanisms. Melatonin increased the fertilization rate in patients whose fertilization rates in the previous cycle were less than 50%, but not in patients whose fertilization rates were more than 50% in the previous cycle. Melatonin increased the blastocyst formation rate in patients whose embryo development rates in the previous cycle were less than 50%, but not in patients whose embryo development rates were more than 50% in the previous cycle. To clarify its mechanisms, transcriptome changes by melatonin treatment in granulosa cells (GCs) of the patients were examined by RNA-sequence. Melatonin treatment altered the transcriptomes of GCs of patients with poor ART outcomes so that they were similar to the transcriptomes of patients with good ART outcomes. The altered genes were associated with the inhibition of cell death and T-cell activity, and the activation of steroidogenesis and angiogenesis. Melatonin treatment was effective for patients with poor fertilization rates and poor embryo development rates in the previous ART cycle. Melatonin alters the GCs transcriptome and, thus, their functions, and this could improve the oocyte quality, leading to good ART outcomes.https://www.mdpi.com/1422-0067/23/12/6731melatoninARTgranulosa cellstranscriptome |
| spellingShingle | Isao Tamura Hiroshi Tamura Mai Kawamoto-Jozaki Yuichiro Shirafuta Taishi Fujimura Yumiko Doi-Tanaka Yumiko Mihara Toshiaki Taketani Norihiro Sugino Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation International Journal of Molecular Sciences melatonin ART granulosa cells transcriptome |
| title | Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation |
| title_full | Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation |
| title_fullStr | Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation |
| title_full_unstemmed | Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation |
| title_short | Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation |
| title_sort | effects of melatonin on the transcriptome of human granulosa cells fertilization and blastocyst formation |
| topic | melatonin ART granulosa cells transcriptome |
| url | https://www.mdpi.com/1422-0067/23/12/6731 |
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