Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm
We report an outbreak of a novel reassortant epizootic hemorrhagic disease virus serotype 6 (EHDV-6) in white-tailed deer (WTD) on a Florida farm in 2019. At necropsy, most animals exhibited hemorrhagic lesions in the lung and heart, and congestion in the lung, liver, and spleen. Histopathology reve...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/1012 |
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| author | Thaís C. S. Rodrigues Pedro H. O. Viadanna Kuttichantran Subramaniam Ian K. Hawkins Albert B. Jeon Julia C. Loeb Juan M. C. Krauer John A. Lednicky Samantha M. Wisely Thomas B. Waltzek |
| author_facet | Thaís C. S. Rodrigues Pedro H. O. Viadanna Kuttichantran Subramaniam Ian K. Hawkins Albert B. Jeon Julia C. Loeb Juan M. C. Krauer John A. Lednicky Samantha M. Wisely Thomas B. Waltzek |
| author_sort | Thaís C. S. Rodrigues |
| collection | DOAJ |
| description | We report an outbreak of a novel reassortant epizootic hemorrhagic disease virus serotype 6 (EHDV-6) in white-tailed deer (WTD) on a Florida farm in 2019. At necropsy, most animals exhibited hemorrhagic lesions in the lung and heart, and congestion in the lung, liver, and spleen. Histopathology revealed multi-organ hemorrhage and congestion, and renal tubular necrosis. Tissues were screened by RT-qPCR and all animals tested positive for EHDV. Tissues were processed for virus isolation and next-generation sequencing was performed on cDNA libraries generated from the RNA extracts of cultures displaying cytopathic effects. Six isolates yielded nearly identical complete genome sequences of a novel U.S. EHDV-6 strain. Genetic and phylogenetic analyses revealed the novel strain to be most closely related to a reassortant EHDV-6 strain isolated from cattle in Trinidad and both strains received segment 4 from an Australian EHDV-2 strain. The novel U.S. EHDV-6 strain is unique in that it acquired segment 8 from an Australian EHDV-8 strain. An RNAscope<sup>®</sup> in situ hybridization assay was developed against the novel U.S. EHDV-6 strain and labeling was detected within lesions of the heart, kidney, liver, and lung. These data support the novel U.S. reassortant EHDV-6 strain as the cause of disease in the farmed WTD. |
| first_indexed | 2024-03-10T01:37:02Z |
| format | Article |
| id | doaj.art-fb420fa307144a2aa8f18b804a3c467e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:37:02Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fb420fa307144a2aa8f18b804a3c467e2023-11-23T13:31:48ZengMDPI AGViruses1999-49152022-05-01145101210.3390/v14051012Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida FarmThaís C. S. Rodrigues0Pedro H. O. Viadanna1Kuttichantran Subramaniam2Ian K. Hawkins3Albert B. Jeon4Julia C. Loeb5Juan M. C. Krauer6John A. Lednicky7Samantha M. Wisely8Thomas B. Waltzek9Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USAEmerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USADepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USAEmerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USAEmerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USAWe report an outbreak of a novel reassortant epizootic hemorrhagic disease virus serotype 6 (EHDV-6) in white-tailed deer (WTD) on a Florida farm in 2019. At necropsy, most animals exhibited hemorrhagic lesions in the lung and heart, and congestion in the lung, liver, and spleen. Histopathology revealed multi-organ hemorrhage and congestion, and renal tubular necrosis. Tissues were screened by RT-qPCR and all animals tested positive for EHDV. Tissues were processed for virus isolation and next-generation sequencing was performed on cDNA libraries generated from the RNA extracts of cultures displaying cytopathic effects. Six isolates yielded nearly identical complete genome sequences of a novel U.S. EHDV-6 strain. Genetic and phylogenetic analyses revealed the novel strain to be most closely related to a reassortant EHDV-6 strain isolated from cattle in Trinidad and both strains received segment 4 from an Australian EHDV-2 strain. The novel U.S. EHDV-6 strain is unique in that it acquired segment 8 from an Australian EHDV-8 strain. An RNAscope<sup>®</sup> in situ hybridization assay was developed against the novel U.S. EHDV-6 strain and labeling was detected within lesions of the heart, kidney, liver, and lung. These data support the novel U.S. reassortant EHDV-6 strain as the cause of disease in the farmed WTD.https://www.mdpi.com/1999-4915/14/5/1012Cervidaeepizootic hemorrhagic disease viruswhite-tailed deerdiseasephylogenyorbivirus |
| spellingShingle | Thaís C. S. Rodrigues Pedro H. O. Viadanna Kuttichantran Subramaniam Ian K. Hawkins Albert B. Jeon Julia C. Loeb Juan M. C. Krauer John A. Lednicky Samantha M. Wisely Thomas B. Waltzek Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm Viruses Cervidae epizootic hemorrhagic disease virus white-tailed deer disease phylogeny orbivirus |
| title | Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm |
| title_full | Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm |
| title_fullStr | Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm |
| title_full_unstemmed | Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm |
| title_short | Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (<i>Odocoileus virginianus</i>) on a Florida Farm |
| title_sort | characterization of a novel reassortant epizootic hemorrhagic disease virus serotype 6 strain isolated from diseased white tailed deer i odocoileus virginianus i on a florida farm |
| topic | Cervidae epizootic hemorrhagic disease virus white-tailed deer disease phylogeny orbivirus |
| url | https://www.mdpi.com/1999-4915/14/5/1012 |
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