Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs
Summary Introduction: Radiotherapy (RT) of head and neck neoplasms often damages the salivary glands. Aim: To examine the pattern of morphologic changes resulting from RT of the head and neck region in minipig parotid glands in a clinical and experimental research setting....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Revinter Publicações Ltda.
2013-04-01
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| Series: | International Archives of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.7162/S1809-97772013000200008 |
| _version_ | 1819088487367835648 |
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| author | Roberta Targa Stramandinoli-Zanicotti Laurindo Moacir Sassi Juliana Lucena Schussel Maria Fernanda Torres Melissa Funchal Gustavo Henrique Smaniotto José Luis Dissenha Andre Lopes Carvalho |
| author_facet | Roberta Targa Stramandinoli-Zanicotti Laurindo Moacir Sassi Juliana Lucena Schussel Maria Fernanda Torres Melissa Funchal Gustavo Henrique Smaniotto José Luis Dissenha Andre Lopes Carvalho |
| author_sort | Roberta Targa Stramandinoli-Zanicotti |
| collection | DOAJ |
| description | Summary
Introduction: Radiotherapy (RT) of head and neck neoplasms often damages the salivary glands.
Aim: To examine the pattern of morphologic changes resulting from RT of the head and neck region in minipig parotid glands in a clinical and experimental research setting.
Methods: Twelve 18-month-old male Brazilian minipigs weighing 30–40 kg were selected. Eight minipigs were assigned to the experimental group (group 1) and 4 to the control group (group 2). The RT was performed under general anesthesia at Erasto Gaertner Hospital, Curitiba, Brazil, using an á/â ratio of 2.5. The minipigs from group 1 underwent 3 sessions of irradiation with Cobalt 60 of the head and neck, bilaterally, with 3 exposures of 8 Gy each at 7-day intervals for a total dose of 24 Gy. The animals were sacrificed 12 weeks post-RT.
Results: The irradiated parotid glands displayed reductions in the size and number of acini as well as loss of secretory granules. The presence of fibrosis and loss of parenchyma relative to non-irradiated glands were observed, with an average reduction in volume of 54%.
Conclusions: Our results demonstrate that this model for parotid gland damage resulting from an RT regimen appears to be useful for preclinical large animal studies of RT-induced damage and testing novel potential treatment options. Although recent advances in radiation therapy, such as intensity-modulated radiation therapy, have reduced the dose and limited the field of radiation, considerable salivary gland injury still occurs and can greatly impact the patient's quality of life after cancer treatment. |
| first_indexed | 2024-12-21T21:52:49Z |
| format | Article |
| id | doaj.art-fb461d37b67b480cb970444daeacc367 |
| institution | Directory Open Access Journal |
| issn | 1809-9777 1809-4864 |
| language | English |
| last_indexed | 2024-12-21T21:52:49Z |
| publishDate | 2013-04-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | International Archives of Otorhinolaryngology |
| spelling | doaj.art-fb461d37b67b480cb970444daeacc3672022-12-21T18:49:03ZengThieme Revinter Publicações Ltda.International Archives of Otorhinolaryngology1809-97771809-48642013-04-01170216316710.7162/S1809-97772013000200008Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigsRoberta Targa Stramandinoli-Zanicotti0Laurindo Moacir Sassi1Juliana Lucena Schussel2Maria Fernanda Torres3Melissa Funchal4Gustavo Henrique Smaniotto5José Luis Dissenha6Andre Lopes Carvalho7DDS, MSc, PhD student. Oral and Maxillofacial Surgery Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil; Oncology Department, University of São Paulo, São Paulo / SP - Brazil.DDS, PhD. Chief of the Oral and Maxillofacial Surgery Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil.DDS, PhD. Oral and Maxillofacial Surgery Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil.DVM, PhD. Experimental Surgery Department, Positivo University, Curitiba / PR - Brazil; Veterinary Medicine of Federal University of Parana, Curitiba / PR - Brazil.Medical Physics. Radiotherapy Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil.MD. Radiotherapy Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil.DDS. Oral and Maxillofacial Surgery Department, Erasto Gaertner Hospital, Curitiba / PR - Brazil.MD, PhD. Head and Neck Surgery and Otorhinolaryngology Department, Hospital de Cancer de Barretos, Barretos, SP, Brazil; Oncology Department, University of São Paulo, São Paulo / SP - Brazil.Summary Introduction: Radiotherapy (RT) of head and neck neoplasms often damages the salivary glands. Aim: To examine the pattern of morphologic changes resulting from RT of the head and neck region in minipig parotid glands in a clinical and experimental research setting. Methods: Twelve 18-month-old male Brazilian minipigs weighing 30–40 kg were selected. Eight minipigs were assigned to the experimental group (group 1) and 4 to the control group (group 2). The RT was performed under general anesthesia at Erasto Gaertner Hospital, Curitiba, Brazil, using an á/â ratio of 2.5. The minipigs from group 1 underwent 3 sessions of irradiation with Cobalt 60 of the head and neck, bilaterally, with 3 exposures of 8 Gy each at 7-day intervals for a total dose of 24 Gy. The animals were sacrificed 12 weeks post-RT. Results: The irradiated parotid glands displayed reductions in the size and number of acini as well as loss of secretory granules. The presence of fibrosis and loss of parenchyma relative to non-irradiated glands were observed, with an average reduction in volume of 54%. Conclusions: Our results demonstrate that this model for parotid gland damage resulting from an RT regimen appears to be useful for preclinical large animal studies of RT-induced damage and testing novel potential treatment options. Although recent advances in radiation therapy, such as intensity-modulated radiation therapy, have reduced the dose and limited the field of radiation, considerable salivary gland injury still occurs and can greatly impact the patient's quality of life after cancer treatment.http://www.thieme-connect.de/DOI/DOI?10.7162/S1809-97772013000200008head and neck neoplasmsradiotherapysalivary glandsxerostomiaswineminiature |
| spellingShingle | Roberta Targa Stramandinoli-Zanicotti Laurindo Moacir Sassi Juliana Lucena Schussel Maria Fernanda Torres Melissa Funchal Gustavo Henrique Smaniotto José Luis Dissenha Andre Lopes Carvalho Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs International Archives of Otorhinolaryngology head and neck neoplasms radiotherapy salivary glands xerostomia swine miniature |
| title | Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs |
| title_full | Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs |
| title_fullStr | Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs |
| title_full_unstemmed | Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs |
| title_short | Effect of fractionated radiotherapy on the parotid gland: an experimental study in Brazilian minipigs |
| title_sort | effect of fractionated radiotherapy on the parotid gland an experimental study in brazilian minipigs |
| topic | head and neck neoplasms radiotherapy salivary glands xerostomia swine miniature |
| url | http://www.thieme-connect.de/DOI/DOI?10.7162/S1809-97772013000200008 |
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