Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey
An epidemiological survey was conducted in south east Mexico, in an effort to establish the serological reactivity and carrier status to Babesia bigemina of an indigenous cattle population. The prevalance was obtained through the Indirect Fluorescent Antibody Test (IFAT), using an in vitro culture-d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1992-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700035 |
| _version_ | 1827842629843288064 |
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| author | Juan A. Ramos Jesus A. Alvarez Julio V. Figueroa Jose Solis Roger I. Rodriguez Ruben Hernandez Gerald M. Buening Carlos A. Vega |
| author_facet | Juan A. Ramos Jesus A. Alvarez Julio V. Figueroa Jose Solis Roger I. Rodriguez Ruben Hernandez Gerald M. Buening Carlos A. Vega |
| author_sort | Juan A. Ramos |
| collection | DOAJ |
| description | An epidemiological survey was conducted in south east Mexico, in an effort to establish the serological reactivity and carrier status to Babesia bigemina of an indigenous cattle population. The prevalance was obtained through the Indirect Fluorescent Antibody Test (IFAT), using an in vitro culture-derived B. bigemina antigen. A specific, digoxigenin-coupled, ~6kb B. bigemina-DNA probe (BBDP), was used to indicate the presence of the parasite. Serum samples from 925 animals of all ages, were obtained within the three regions (I, II, III) of the state of Yucatan and tested by IFAT. In addition, whole blood samples draw from 136 of the same animals of region II were analyzed using the BBDP. Positive IFAT (IFAT+) reactions were observed in 531 sera for a 57% overall prevalence. Regional values were: I = 157 + (56%), II = 266 + (68%) and III = 108 + (42%). Only 32 (23%) of the blood samples tested with BBDP showed distinctive hybridization signal, in contrast with 100 (73%) IFAT + animals. The responses distribution for IFAT vs. BBDP was: +/+ 23, +/- 77, -/+ 9 and -/- 27 respectively. It was found that the analytical sinsitivity of BBDP appears to be low for its utilization is widespread epidemiological surveys. It was considered, however, that the colorimetric probe mifht to be useful to safely detect transmission prone carriers, since it is able to detect parasitemias as low as 0.001%. |
| first_indexed | 2024-03-12T08:13:29Z |
| format | Article |
| id | doaj.art-fb46a8102f594c7b9594d2692282e89e |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:13:29Z |
| publishDate | 1992-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-fb46a8102f594c7b9594d2692282e89e2023-09-02T19:06:34ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601992-01-018721321710.1590/S0074-02761992000700035Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological surveyJuan A. RamosJesus A. AlvarezJulio V. FigueroaJose SolisRoger I. RodriguezRuben HernandezGerald M. BueningCarlos A. VegaAn epidemiological survey was conducted in south east Mexico, in an effort to establish the serological reactivity and carrier status to Babesia bigemina of an indigenous cattle population. The prevalance was obtained through the Indirect Fluorescent Antibody Test (IFAT), using an in vitro culture-derived B. bigemina antigen. A specific, digoxigenin-coupled, ~6kb B. bigemina-DNA probe (BBDP), was used to indicate the presence of the parasite. Serum samples from 925 animals of all ages, were obtained within the three regions (I, II, III) of the state of Yucatan and tested by IFAT. In addition, whole blood samples draw from 136 of the same animals of region II were analyzed using the BBDP. Positive IFAT (IFAT+) reactions were observed in 531 sera for a 57% overall prevalence. Regional values were: I = 157 + (56%), II = 266 + (68%) and III = 108 + (42%). Only 32 (23%) of the blood samples tested with BBDP showed distinctive hybridization signal, in contrast with 100 (73%) IFAT + animals. The responses distribution for IFAT vs. BBDP was: +/+ 23, +/- 77, -/+ 9 and -/- 27 respectively. It was found that the analytical sinsitivity of BBDP appears to be low for its utilization is widespread epidemiological surveys. It was considered, however, that the colorimetric probe mifht to be useful to safely detect transmission prone carriers, since it is able to detect parasitemias as low as 0.001%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700035Babesia bigeminaDNA probeEpidemiologyIFAT |
| spellingShingle | Juan A. Ramos Jesus A. Alvarez Julio V. Figueroa Jose Solis Roger I. Rodriguez Ruben Hernandez Gerald M. Buening Carlos A. Vega Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey Memorias do Instituto Oswaldo Cruz Babesia bigemina DNA probe Epidemiology IFAT |
| title | Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey |
| title_full | Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey |
| title_fullStr | Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey |
| title_full_unstemmed | Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey |
| title_short | Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey |
| title_sort | evaluation of a colorimetric babesia bigemina dna probe within an epidemiological survey |
| topic | Babesia bigemina DNA probe Epidemiology IFAT |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700035 |
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