Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B
SaNa-1A is a novel cytoplasmic male sterility (CMS) line in Brassica napus derived from progenies of somatic hybrids between B. napus and Sinapis alba, and SaNa-1B is the corresponding maintainer line. In this study, phenotypic differences of floral organs between CMS and the maintainer lines were o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01313/full |
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| author | Kun Du Qier Liu Xinyue Wu Jinjin Jiang Jian Wu Yujie Fang Aimin Li Youping Wang |
| author_facet | Kun Du Qier Liu Xinyue Wu Jinjin Jiang Jian Wu Yujie Fang Aimin Li Youping Wang |
| author_sort | Kun Du |
| collection | DOAJ |
| description | SaNa-1A is a novel cytoplasmic male sterility (CMS) line in Brassica napus derived from progenies of somatic hybrids between B. napus and Sinapis alba, and SaNa-1B is the corresponding maintainer line. In this study, phenotypic differences of floral organs between CMS and the maintainer lines were observed. By microscope observation in different anther developmental stages of two lines, we found the anther development in SaNa-1A was abnormal since the tetrad stage, and microspore development was ceased during the uninucleate stage. Transcriptomic sequencing for floral buds of sterile and fertile plants were conducted to elucidate gene expression and regulation caused by the alien chromosome and cytoplasm from S. alba. Clean tags obtained were assembled into 195,568 unigenes, and 7,811 unigenes distributed in the metabolic and protein synthesis pathways were identified with significant expression differences between two libraries. We also observed that genes participating in carbon metabolism, tricarboxylic acid cycle, oxidative phosphorylation, oxidation–reduction system, pentatricopeptide repeat, and anther development were downregulated in the sterile line. Some of them are candidates for researches on the sterility mechanism in the CMS material, fertility restoration, and improvement of economic traits in the maintainer line. Further research on the tags with expressional specificity in the fertile line would be helpful to explore desirable agronomic traits from wild species of rapeseed. |
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| id | doaj.art-fb484953541d4f5b91d93bbc9b973e5e |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T01:13:13Z |
| publishDate | 2016-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fb484953541d4f5b91d93bbc9b973e5e2022-12-22T01:25:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-09-01710.3389/fpls.2016.01313218298Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1BKun Du0Qier Liu1Xinyue Wu2Jinjin Jiang3Jian Wu4Yujie Fang5Aimin Li6Youping Wang7Yangzhou UniversityYangzhou UniversityYangzhou UniversityYangzhou UniversityYangzhou UniversityYangzhou UniversityJiangsu Institute of Agricultural Science in the Lixiahe DistrictYangzhou UniversitySaNa-1A is a novel cytoplasmic male sterility (CMS) line in Brassica napus derived from progenies of somatic hybrids between B. napus and Sinapis alba, and SaNa-1B is the corresponding maintainer line. In this study, phenotypic differences of floral organs between CMS and the maintainer lines were observed. By microscope observation in different anther developmental stages of two lines, we found the anther development in SaNa-1A was abnormal since the tetrad stage, and microspore development was ceased during the uninucleate stage. Transcriptomic sequencing for floral buds of sterile and fertile plants were conducted to elucidate gene expression and regulation caused by the alien chromosome and cytoplasm from S. alba. Clean tags obtained were assembled into 195,568 unigenes, and 7,811 unigenes distributed in the metabolic and protein synthesis pathways were identified with significant expression differences between two libraries. We also observed that genes participating in carbon metabolism, tricarboxylic acid cycle, oxidative phosphorylation, oxidation–reduction system, pentatricopeptide repeat, and anther development were downregulated in the sterile line. Some of them are candidates for researches on the sterility mechanism in the CMS material, fertility restoration, and improvement of economic traits in the maintainer line. Further research on the tags with expressional specificity in the fertile line would be helpful to explore desirable agronomic traits from wild species of rapeseed.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01313/fullBrassica napusTranscription, GeneticMorphological structureCytoplasmic male sterility (CMS)Somatic hybridization |
| spellingShingle | Kun Du Qier Liu Xinyue Wu Jinjin Jiang Jian Wu Yujie Fang Aimin Li Youping Wang Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B Frontiers in Plant Science Brassica napus Transcription, Genetic Morphological structure Cytoplasmic male sterility (CMS) Somatic hybridization |
| title | Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B |
| title_full | Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B |
| title_fullStr | Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B |
| title_full_unstemmed | Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B |
| title_short | Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1A) derived from somatic hybridization and its maintainer line SaNa-1B |
| title_sort | morphological structure and transcriptome comparison of the cytoplasmic male sterility line in brassica napus sana 1a derived from somatic hybridization and its maintainer line sana 1b |
| topic | Brassica napus Transcription, Genetic Morphological structure Cytoplasmic male sterility (CMS) Somatic hybridization |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01313/full |
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