Multiplex secretome engineering enhances recombinant protein production and purity
Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-15866-w |
| _version_ | 1818851465048883200 |
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| author | Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis |
| author_facet | Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis |
| author_sort | Stefan Kol |
| collection | DOAJ |
| description | Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity. |
| first_indexed | 2024-12-19T07:05:27Z |
| format | Article |
| id | doaj.art-fb48699f0dc94c81a1ca056457c7fde6 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T07:05:27Z |
| publishDate | 2020-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fb48699f0dc94c81a1ca056457c7fde62022-12-21T20:31:17ZengNature PortfolioNature Communications2041-17232020-04-0111111010.1038/s41467-020-15866-wMultiplex secretome engineering enhances recombinant protein production and purityStefan Kol0Daniel Ley1Tune Wulff2Marianne Decker3Johnny Arnsdorf4Sanne Schoffelen5Anders Holmgaard Hansen6Tanja Lyholm Jensen7Jahir M. Gutierrez8Austin W. T. Chiang9Helen O. Masson10Bernhard O. Palsson11Bjørn G. Voldborg12Lasse Ebdrup Pedersen13Helene Faustrup Kildegaard14Gyun Min Lee15Nathan E. Lewis16The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of MedicineThe Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of MedicineThe Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of MedicineThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, KemitorvetThe Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of MedicineHost cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.https://doi.org/10.1038/s41467-020-15866-w |
| spellingShingle | Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis Multiplex secretome engineering enhances recombinant protein production and purity Nature Communications |
| title | Multiplex secretome engineering enhances recombinant protein production and purity |
| title_full | Multiplex secretome engineering enhances recombinant protein production and purity |
| title_fullStr | Multiplex secretome engineering enhances recombinant protein production and purity |
| title_full_unstemmed | Multiplex secretome engineering enhances recombinant protein production and purity |
| title_short | Multiplex secretome engineering enhances recombinant protein production and purity |
| title_sort | multiplex secretome engineering enhances recombinant protein production and purity |
| url | https://doi.org/10.1038/s41467-020-15866-w |
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