Genome-wide association study of vitamin E in sweet corn kernels
Vitamin E, consisting of tocopherols and tocotrienols, serves as a lipid-soluble antioxidant in sweet corn kernels, providing nutrients to both plants and humans. Though the key genes involved in the vitamin E biosynthesis pathway have been identified in plants, the genetic architecture of vitamin E...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-04-01
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| Series: | Crop Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514119301163 |
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| author | Yingni Xiao Yongtao Yu Gaoke Li Lihua Xie Xinbo Guo Jiansheng Li Yuliang Li Jianguang Hu |
| author_facet | Yingni Xiao Yongtao Yu Gaoke Li Lihua Xie Xinbo Guo Jiansheng Li Yuliang Li Jianguang Hu |
| author_sort | Yingni Xiao |
| collection | DOAJ |
| description | Vitamin E, consisting of tocopherols and tocotrienols, serves as a lipid-soluble antioxidant in sweet corn kernels, providing nutrients to both plants and humans. Though the key genes involved in the vitamin E biosynthesis pathway have been identified in plants, the genetic architecture of vitamin E content in sweet corn kernels remains largely unclear. In the present study, an association panel of 204 inbred lines of sweet corn was constructed. Seven compounds of vitamin E were quantified in sweet corn kernels at 28 days after pollination. A total of 119 loci for vitamin E were identified using a genome-wide association study based on genotyping by sequencing, and a genetic network of vitamin E was constructed. Candidate genes identified were involved mainly in RNA regulation and protein metabolism. The known gene ZmVTE4, encoding γ-tocopherol methyltransferase, was significantly associated with four traits (α-tocopherol, α-tocotrienol, the α/γ-tocopherol ratio, and the α/γ-tocotrienol ratio). The effects of two causative markers on ZmVTE4 were validated by haplotype analysis. Finally, two elite cultivars (Yuetian 9 and Yuetian 22) with a 4.5-fold increase in the sum of α- and γ-tocopherols were developed by marker-assisted selection, demonstrating the successful biofortification of sweet corn. Three genes (DAHPS, ADT2, and cmu2) involved in chorismate and tyrosine synthesis were significantly associated with the α/γ-tocotrienol ratio. These results shed light on the genetic architecture of vitamin E and may accelerate the nutritional improvement of sweet corn. Keywords: Sweet corn, GBS, Genome-wide association study, Vitamin E, Marker-assisted selection |
| first_indexed | 2024-12-19T10:57:29Z |
| format | Article |
| id | doaj.art-fb48fc2e559646b4b31eb60e360a967f |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-19T10:57:29Z |
| publishDate | 2020-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-fb48fc2e559646b4b31eb60e360a967f2022-12-21T20:24:46ZengKeAi Communications Co., Ltd.Crop Journal2214-51412020-04-0182341350Genome-wide association study of vitamin E in sweet corn kernelsYingni Xiao0Yongtao Yu1Gaoke Li2Lihua Xie3Xinbo Guo4Jiansheng Li5Yuliang Li6Jianguang Hu7Crop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, ChinaCrop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, ChinaCrop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, ChinaCrop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong, ChinaNational Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaCrop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, ChinaCrop Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Crops Genetics and Improvement of Guangdong Province, Guangzhou 510640, Guangdong, China; Corresponding author.Vitamin E, consisting of tocopherols and tocotrienols, serves as a lipid-soluble antioxidant in sweet corn kernels, providing nutrients to both plants and humans. Though the key genes involved in the vitamin E biosynthesis pathway have been identified in plants, the genetic architecture of vitamin E content in sweet corn kernels remains largely unclear. In the present study, an association panel of 204 inbred lines of sweet corn was constructed. Seven compounds of vitamin E were quantified in sweet corn kernels at 28 days after pollination. A total of 119 loci for vitamin E were identified using a genome-wide association study based on genotyping by sequencing, and a genetic network of vitamin E was constructed. Candidate genes identified were involved mainly in RNA regulation and protein metabolism. The known gene ZmVTE4, encoding γ-tocopherol methyltransferase, was significantly associated with four traits (α-tocopherol, α-tocotrienol, the α/γ-tocopherol ratio, and the α/γ-tocotrienol ratio). The effects of two causative markers on ZmVTE4 were validated by haplotype analysis. Finally, two elite cultivars (Yuetian 9 and Yuetian 22) with a 4.5-fold increase in the sum of α- and γ-tocopherols were developed by marker-assisted selection, demonstrating the successful biofortification of sweet corn. Three genes (DAHPS, ADT2, and cmu2) involved in chorismate and tyrosine synthesis were significantly associated with the α/γ-tocotrienol ratio. These results shed light on the genetic architecture of vitamin E and may accelerate the nutritional improvement of sweet corn. Keywords: Sweet corn, GBS, Genome-wide association study, Vitamin E, Marker-assisted selectionhttp://www.sciencedirect.com/science/article/pii/S2214514119301163 |
| spellingShingle | Yingni Xiao Yongtao Yu Gaoke Li Lihua Xie Xinbo Guo Jiansheng Li Yuliang Li Jianguang Hu Genome-wide association study of vitamin E in sweet corn kernels Crop Journal |
| title | Genome-wide association study of vitamin E in sweet corn kernels |
| title_full | Genome-wide association study of vitamin E in sweet corn kernels |
| title_fullStr | Genome-wide association study of vitamin E in sweet corn kernels |
| title_full_unstemmed | Genome-wide association study of vitamin E in sweet corn kernels |
| title_short | Genome-wide association study of vitamin E in sweet corn kernels |
| title_sort | genome wide association study of vitamin e in sweet corn kernels |
| url | http://www.sciencedirect.com/science/article/pii/S2214514119301163 |
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