Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i>
Bistable expression of the <i>Salmonella enterica</i> pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the flagellar regulon reveals independent switching, with conco...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/2/210 |
| _version_ | 1797409298856280064 |
|---|---|
| author | María Antonia Sánchez-Romero Josep Casadesús |
| author_facet | María Antonia Sánchez-Romero Josep Casadesús |
| author_sort | María Antonia Sánchez-Romero |
| collection | DOAJ |
| description | Bistable expression of the <i>Salmonella enterica</i> pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the flagellar regulon reveals independent switching, with concomitant formation of four subpopulations: SPI-1<sup>OFF</sup> Flag<sup>OFF</sup>, SPI-1<sup>OFF</sup> Flag<sup>ON</sup>, SPI-1<sup>ON</sup> Flag<sup>OFF</sup>, and SPI-1<sup>ON</sup> Flag<sup>ON</sup>. Invasion assays upon cell sorting show that none of the four subpopulations is highly invasive, thus raising the possibility that Flag<sup>OFF</sup> cells might contribute to optimal invasion as previously proposed for SPI-1<sup>OFF</sup> cells. Time lapse microscopy observation indicates that expression of the flagellar regulon contributes to the growth impairment previously described in SPI-1<sup>ON</sup> cells. As a consequence, growth resumption in SPI-1<sup>ON</sup> Flag<sup>ON</sup> cells requires switching to both SPI-1<sup>OFF</sup> and Flag<sup>OFF</sup> states. |
| first_indexed | 2024-03-09T04:12:16Z |
| format | Article |
| id | doaj.art-fb4c66771fef4574b2202e93a0dbe8f0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:12:16Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fb4c66771fef4574b2202e93a0dbe8f02023-12-03T13:59:38ZengMDPI AGMicroorganisms2076-26072021-01-019221010.3390/microorganisms9020210Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i>María Antonia Sánchez-Romero0Josep Casadesús1Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Calle Profesor García González, 2, 41012 Seville, SpainDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, 41080 Seville, SpainBistable expression of the <i>Salmonella enterica</i> pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the flagellar regulon reveals independent switching, with concomitant formation of four subpopulations: SPI-1<sup>OFF</sup> Flag<sup>OFF</sup>, SPI-1<sup>OFF</sup> Flag<sup>ON</sup>, SPI-1<sup>ON</sup> Flag<sup>OFF</sup>, and SPI-1<sup>ON</sup> Flag<sup>ON</sup>. Invasion assays upon cell sorting show that none of the four subpopulations is highly invasive, thus raising the possibility that Flag<sup>OFF</sup> cells might contribute to optimal invasion as previously proposed for SPI-1<sup>OFF</sup> cells. Time lapse microscopy observation indicates that expression of the flagellar regulon contributes to the growth impairment previously described in SPI-1<sup>ON</sup> cells. As a consequence, growth resumption in SPI-1<sup>ON</sup> Flag<sup>ON</sup> cells requires switching to both SPI-1<sup>OFF</sup> and Flag<sup>OFF</sup> states.https://www.mdpi.com/2076-2607/9/2/210phenotypic heterogeneitybistabilityepithelial cell invasionSPI-1flagellar regulon |
| spellingShingle | María Antonia Sánchez-Romero Josep Casadesús Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> Microorganisms phenotypic heterogeneity bistability epithelial cell invasion SPI-1 flagellar regulon |
| title | Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> |
| title_full | Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> |
| title_fullStr | Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> |
| title_full_unstemmed | Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> |
| title_short | Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in <i>Salmonella enterica</i> |
| title_sort | single cell analysis of bistable expression of pathogenicity island 1 and the flagellar regulon in i salmonella enterica i |
| topic | phenotypic heterogeneity bistability epithelial cell invasion SPI-1 flagellar regulon |
| url | https://www.mdpi.com/2076-2607/9/2/210 |
| work_keys_str_mv | AT mariaantoniasanchezromero singlecellanalysisofbistableexpressionofpathogenicityisland1andtheflagellarreguloninisalmonellaentericai AT josepcasadesus singlecellanalysisofbistableexpressionofpathogenicityisland1andtheflagellarreguloninisalmonellaentericai |