Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology
<p>Abstract</p> <p>Background</p> <p>Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we develo...
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| Format: | Article |
| Language: | English |
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BMC
2003-07-01
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| Series: | BMC Cell Biology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2121/4/6 |
| _version_ | 1818526345133555712 |
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| author | Ingber Donald E Huang Sui Brock Amy |
| author_facet | Ingber Donald E Huang Sui Brock Amy |
| author_sort | Ingber Donald E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner.</p> <p>Results</p> <p>Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype.</p> <p>Conclusions</p> <p>We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.</p> |
| first_indexed | 2024-12-11T06:21:42Z |
| format | Article |
| id | doaj.art-fb4f060aa2014dc19fdd3cf08d8759b1 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-11T06:21:42Z |
| publishDate | 2003-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-fb4f060aa2014dc19fdd3cf08d8759b12022-12-22T01:17:49ZengBMCBMC Cell Biology1471-21212003-07-0141610.1186/1471-2121-4-6Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technologyIngber Donald EHuang SuiBrock Amy<p>Abstract</p> <p>Background</p> <p>Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner.</p> <p>Results</p> <p>Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype.</p> <p>Conclusions</p> <p>We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.</p>http://www.biomedcentral.com/1471-2121/4/6mRNA localizationgene expressiongene profilingactincytoskeletonpost-transcriptional controlsubcellular localization |
| spellingShingle | Ingber Donald E Huang Sui Brock Amy Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology BMC Cell Biology mRNA localization gene expression gene profiling actin cytoskeleton post-transcriptional control subcellular localization |
| title | Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology |
| title_full | Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology |
| title_fullStr | Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology |
| title_full_unstemmed | Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology |
| title_short | Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology |
| title_sort | identification of a distinct class of cytoskeleton associated mrnas using microarray technology |
| topic | mRNA localization gene expression gene profiling actin cytoskeleton post-transcriptional control subcellular localization |
| url | http://www.biomedcentral.com/1471-2121/4/6 |
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