Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley
The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp d...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2010-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0002_allelic-variations-at-dhn4-and-dhn7-are-associated-with-frost-tolerance-in-barley.php |
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| author | Ludmila Holková Pavlína Mikulková Pavlína Hrstková Ilja Tom Prášil Marta Bradáčová Pavla Prášilová Oldřich Chloupek |
| author_facet | Ludmila Holková Pavlína Mikulková Pavlína Hrstková Ilja Tom Prášil Marta Bradáčová Pavla Prášilová Oldřich Chloupek |
| author_sort | Ludmila Holková |
| collection | DOAJ |
| description | The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from -10.0°C to -14.3°C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combination of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant. |
| first_indexed | 2024-04-10T08:30:03Z |
| format | Article |
| id | doaj.art-fb55e825b2e34ebb897f7e8290aca1e4 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:30:03Z |
| publishDate | 2010-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-fb55e825b2e34ebb897f7e8290aca1e42023-02-23T03:30:07ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252010-12-0146414915810.17221/90/2010-CJGPBcjg-201004-0002Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barleyLudmila Holková0Pavlína Mikulková1Pavlína Hrstková2Ilja Tom Prášil3Marta Bradáčová4Pavla Prášilová5Oldřich Chloupek6Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicThe sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from -10.0°C to -14.3°C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combination of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant.https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0002_allelic-variations-at-dhn4-and-dhn7-are-associated-with-frost-tolerance-in-barley.phpabiotic stressdehydrinsgene polymorphismhordeum vulgare |
| spellingShingle | Ludmila Holková Pavlína Mikulková Pavlína Hrstková Ilja Tom Prášil Marta Bradáčová Pavla Prášilová Oldřich Chloupek Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley Czech Journal of Genetics and Plant Breeding abiotic stress dehydrins gene polymorphism hordeum vulgare |
| title | Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley |
| title_full | Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley |
| title_fullStr | Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley |
| title_full_unstemmed | Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley |
| title_short | Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley |
| title_sort | allelic variations at dhn4 and dhn7 are associated with frost tolerance in barley |
| topic | abiotic stress dehydrins gene polymorphism hordeum vulgare |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201004-0002_allelic-variations-at-dhn4-and-dhn7-are-associated-with-frost-tolerance-in-barley.php |
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