SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing
Alternative splicing is a major mechanism of controlling gene expression and protein diversity in higher eukaryotes. We report that the splicing factor SRSF10 functions during striated muscle development, myoblast differentiation, and glucose production both in cells and in mice. A combination of RN...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-11-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715012085 |
| _version_ | 1811269175490904064 |
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| author | Ning Wei Yuanming Cheng Zhijia Wang Yuguo Liu Chunling Luo Lina Liu Linlin Chen Zhiqin Xie Yun Lu Ying Feng |
| author_facet | Ning Wei Yuanming Cheng Zhijia Wang Yuguo Liu Chunling Luo Lina Liu Linlin Chen Zhiqin Xie Yun Lu Ying Feng |
| author_sort | Ning Wei |
| collection | DOAJ |
| description | Alternative splicing is a major mechanism of controlling gene expression and protein diversity in higher eukaryotes. We report that the splicing factor SRSF10 functions during striated muscle development, myoblast differentiation, and glucose production both in cells and in mice. A combination of RNA-sequencing and molecular analysis allowed us to identify muscle-specific splicing events controlled by SRSF10 that are critically involved in striated muscle development. Inclusion of alternative exons 16 and 17 of Lrrfip1 is a muscle-specific event that is activated by SRSF10 and essential for myoblast differentiation. On the other hand, in mouse primary hepatocytes, PGC1α is a key target of SRSF10 that regulates glucose production by fasting. SRSF10 represses inclusion of PGC1α exon 7a and facilitates the production of functional protein. The results highlight the biological significance of SRSF10 and regulated alternative splicing in vivo. |
| first_indexed | 2024-04-12T21:36:15Z |
| format | Article |
| id | doaj.art-fb5628686ff5487cb40003115c2947f0 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T21:36:15Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-fb5628686ff5487cb40003115c2947f02022-12-22T03:15:53ZengElsevierCell Reports2211-12472015-11-011381647165710.1016/j.celrep.2015.10.038SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative SplicingNing Wei0Yuanming Cheng1Zhijia Wang2Yuguo Liu3Chunling Luo4Lina Liu5Linlin Chen6Zhiqin Xie7Yun Lu8Ying Feng9Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaAffiliated Hospital of Qingdao University, Qingdao 266003, ChinaKey Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaAlternative splicing is a major mechanism of controlling gene expression and protein diversity in higher eukaryotes. We report that the splicing factor SRSF10 functions during striated muscle development, myoblast differentiation, and glucose production both in cells and in mice. A combination of RNA-sequencing and molecular analysis allowed us to identify muscle-specific splicing events controlled by SRSF10 that are critically involved in striated muscle development. Inclusion of alternative exons 16 and 17 of Lrrfip1 is a muscle-specific event that is activated by SRSF10 and essential for myoblast differentiation. On the other hand, in mouse primary hepatocytes, PGC1α is a key target of SRSF10 that regulates glucose production by fasting. SRSF10 represses inclusion of PGC1α exon 7a and facilitates the production of functional protein. The results highlight the biological significance of SRSF10 and regulated alternative splicing in vivo.http://www.sciencedirect.com/science/article/pii/S2211124715012085splicing factor SRSF10alternative splicingmyoblast differentiationglucose production |
| spellingShingle | Ning Wei Yuanming Cheng Zhijia Wang Yuguo Liu Chunling Luo Lina Liu Linlin Chen Zhiqin Xie Yun Lu Ying Feng SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing Cell Reports splicing factor SRSF10 alternative splicing myoblast differentiation glucose production |
| title | SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing |
| title_full | SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing |
| title_fullStr | SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing |
| title_full_unstemmed | SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing |
| title_short | SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing |
| title_sort | srsf10 plays a role in myoblast differentiation and glucose production via regulation of alternative splicing |
| topic | splicing factor SRSF10 alternative splicing myoblast differentiation glucose production |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715012085 |
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