Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus
The pro-neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124000102 |
| _version_ | 1797319524306911232 |
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| author | Sara Miellet Mitchell St Clair-Glover Marnie Maddock Mirella Dottori |
| author_facet | Sara Miellet Mitchell St Clair-Glover Marnie Maddock Mirella Dottori |
| author_sort | Sara Miellet |
| collection | DOAJ |
| description | The pro-neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expression construct featuring a floxed Blasticidin/mApple selection module in the safe-harbor locus CLYBL. This cell line retains its pluripotent state in the absence of DOX, yet readily transitions into a neuronal state upon DOX introduction. |
| first_indexed | 2024-03-08T04:08:08Z |
| format | Article |
| id | doaj.art-fb57f7e9858f4f39ad86be25771711f7 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-08T04:08:08Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-fb57f7e9858f4f39ad86be25771711f72024-02-09T04:47:57ZengElsevierStem Cell Research1873-50612024-03-0175103312Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locusSara Miellet0Mitchell St Clair-Glover1Marnie Maddock2Mirella Dottori3Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, AustraliaMolecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, AustraliaMolecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, AustraliaCorresponding author.; Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, AustraliaThe pro-neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expression construct featuring a floxed Blasticidin/mApple selection module in the safe-harbor locus CLYBL. This cell line retains its pluripotent state in the absence of DOX, yet readily transitions into a neuronal state upon DOX introduction.http://www.sciencedirect.com/science/article/pii/S1873506124000102 |
| spellingShingle | Sara Miellet Mitchell St Clair-Glover Marnie Maddock Mirella Dottori Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus Stem Cell Research |
| title | Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus |
| title_full | Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus |
| title_fullStr | Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus |
| title_full_unstemmed | Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus |
| title_short | Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus |
| title_sort | generation of a gene edited h9 embryonic stem cell line carrying a dox inducible ngn2 expression cassette in the clybl locus |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124000102 |
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