Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase
The goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H2O) layers, using...
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MDPI AG
2014-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/9/13212 |
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| author | Hong Mei Li Seung Hwan Hwang Beom Goo Kang Jae Seung Hong Soon Sung Lim |
| author_facet | Hong Mei Li Seung Hwan Hwang Beom Goo Kang Jae Seung Hong Soon Sung Lim |
| author_sort | Hong Mei Li |
| collection | DOAJ |
| description | The goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H2O) layers, using dl-glyceraldehyde as a substrate. Ten compounds, namely tryptophan (1), orientin (2), isoorientin (3), vitexin (4), isovitexin (5), luteolin-7-O-glucoside (6), luteolin-7-O-rutinoside (7), rosmarinic acid (8), 1-O-feruloyl-d-glucoside (9) and 1-O-caffeoyl-d-glucoside (10) were isolated from the EtOAc and BuOH fractions of C. esculenta. The structures of compounds 1–10 were elucidated by spectroscopic methods and comparison with previous reports. All the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity against rat lens aldose reductase. Among tested compounds, compounds 2 and 3 significantly inhibited rat lens aldose reductase, with IC50 values of 1.65 and 1.92 μM, respectively. Notably, the inhibitory activity of orientin was 3.9 times greater than that of the positive control, quercetin (4.12 μM). However, the isolated compounds showed only moderate ABTS+ [2,29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] activity. These results suggest that flavonoid derivatives from Colocasia esculenta (L.) Schott represent potential compounds for the prevention and/or treatment of diabetic complications. |
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| language | English |
| last_indexed | 2024-04-14T02:06:29Z |
| publishDate | 2014-08-01 |
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| series | Molecules |
| spelling | doaj.art-fb5888236e9547bd917926eee4cb2e8c2022-12-22T02:18:40ZengMDPI AGMolecules1420-30492014-08-01199132121322410.3390/molecules190913212molecules190913212Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose ReductaseHong Mei Li0Seung Hwan Hwang1Beom Goo Kang2Jae Seung Hong3Soon Sung Lim4Department of Pharmacology, College of Medicine, Hallym University, 200-702 Chuncheon, KoreaDepartment of Food and Nutrition, Hallym University, 200-702 Chuncheon, KoreaDepartment of Food and Nutrition, Hallym University, 200-702 Chuncheon, KoreaDepartment of Physical Education, Hallym University, 200-702 Chuncheon, KoreaDepartment of Food and Nutrition, Hallym University, 200-702 Chuncheon, KoreaThe goal of this study was to determine the rat lens aldose reductase-inhibitory effects of 95% ethanol extracts from the leaves of C. esculenta and, its organic solvent soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water (H2O) layers, using dl-glyceraldehyde as a substrate. Ten compounds, namely tryptophan (1), orientin (2), isoorientin (3), vitexin (4), isovitexin (5), luteolin-7-O-glucoside (6), luteolin-7-O-rutinoside (7), rosmarinic acid (8), 1-O-feruloyl-d-glucoside (9) and 1-O-caffeoyl-d-glucoside (10) were isolated from the EtOAc and BuOH fractions of C. esculenta. The structures of compounds 1–10 were elucidated by spectroscopic methods and comparison with previous reports. All the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity against rat lens aldose reductase. Among tested compounds, compounds 2 and 3 significantly inhibited rat lens aldose reductase, with IC50 values of 1.65 and 1.92 μM, respectively. Notably, the inhibitory activity of orientin was 3.9 times greater than that of the positive control, quercetin (4.12 μM). However, the isolated compounds showed only moderate ABTS+ [2,29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] activity. These results suggest that flavonoid derivatives from Colocasia esculenta (L.) Schott represent potential compounds for the prevention and/or treatment of diabetic complications.http://www.mdpi.com/1420-3049/19/9/13212Colocasia esculenta (L.) Schottaldose reductaseantioxidantdiabetic complication |
| spellingShingle | Hong Mei Li Seung Hwan Hwang Beom Goo Kang Jae Seung Hong Soon Sung Lim Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase Molecules Colocasia esculenta (L.) Schott aldose reductase antioxidant diabetic complication |
| title | Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase |
| title_full | Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase |
| title_fullStr | Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase |
| title_full_unstemmed | Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase |
| title_short | Inhibitory Effects of Colocasia esculenta (L.) Schott Constituents on Aldose Reductase |
| title_sort | inhibitory effects of colocasia esculenta l schott constituents on aldose reductase |
| topic | Colocasia esculenta (L.) Schott aldose reductase antioxidant diabetic complication |
| url | http://www.mdpi.com/1420-3049/19/9/13212 |
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