Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells
Objective To investigate the role of UBE2N in the radiosensitivity of laryngocarcinoma hep-2 cells. Methods qPCR and Western blot were used to investigate the silencing efficiency of UBE2N siRNA in hep-2 cells. RNA interference was used to inhibit the expression of UBE2N. After being irradiated 0, 2...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2019-07-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.1648.htm |
| _version_ | 1818113911232135168 |
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| author | GONG Mengxiao DENG Xinzhou SHEN Li ZHANG Menglin KE Qing WU Lin LIU Yanqin CHEN Xiaolin QIU Xiaoyan LUO Zhiguo |
| author_facet | GONG Mengxiao DENG Xinzhou SHEN Li ZHANG Menglin KE Qing WU Lin LIU Yanqin CHEN Xiaolin QIU Xiaoyan LUO Zhiguo |
| author_sort | GONG Mengxiao |
| collection | DOAJ |
| description | Objective To investigate the role of UBE2N in the radiosensitivity of laryngocarcinoma hep-2 cells. Methods qPCR and Western blot were used to investigate the silencing efficiency of UBE2N siRNA in hep-2 cells. RNA interference was used to inhibit the expression of UBE2N. After being irradiated 0, 2, 4, 6, and 8 Gy X-rays irradiation, cell proliferation was detected by CCK8 assay, cell cycle and apoptosis were detected by flow cytometry, and the ability of propagation was detected by clonal formation assay. Results UBE2N siRNA significantly inhibited the expression of UBE2N at the mRNA and protein levels in hep-2 cells. UBE2N silence significantly enhanced the proliferation of hep-2 cells. After UBE2N silencing and certain doses of radiation, the proliferation ability of hep-2 cells was enhanced, the S stage proportion was increased while, the G2 stage proportion was decreased, the apoptosis rate was decreased while the clonal formation ability was enhanced, compared with the control group (all P < 0.05). Conclusion As a tumor suppressor gene, UBE2N enhances the radiosensitivity of laryngeal squamous cell carcinoma by regulating cell proliferation, cell cycle, apoptosis and clonal formation ability. |
| first_indexed | 2024-12-11T03:42:21Z |
| format | Article |
| id | doaj.art-fb5befa1ed974644ba583a976019d51c |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-11T03:42:21Z |
| publishDate | 2019-07-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-fb5befa1ed974644ba583a976019d51c2022-12-22T01:22:06ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782019-07-0146758358710.3971/j.issn.1000-8578.2019.18.16488578.2019.18.1648Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 CellsGONG Mengxiao0DENG Xinzhou1SHEN Li2ZHANG Menglin3KE Qing4WU Lin5LIU Yanqin6CHEN Xiaolin7QIU Xiaoyan8LUO Zhiguo9Cancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaCancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Biochemistry, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaSchool of Biological Engineering, Hubei University of Medicine, Shiyan 442000, ChinaCancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaCancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaCancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaThe First Clinical School, Hubei University of Medicine, Shiyan 442000, ChinaThe First Clinical School, Hubei University of Medicine, Shiyan 442000, ChinaCancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaObjective To investigate the role of UBE2N in the radiosensitivity of laryngocarcinoma hep-2 cells. Methods qPCR and Western blot were used to investigate the silencing efficiency of UBE2N siRNA in hep-2 cells. RNA interference was used to inhibit the expression of UBE2N. After being irradiated 0, 2, 4, 6, and 8 Gy X-rays irradiation, cell proliferation was detected by CCK8 assay, cell cycle and apoptosis were detected by flow cytometry, and the ability of propagation was detected by clonal formation assay. Results UBE2N siRNA significantly inhibited the expression of UBE2N at the mRNA and protein levels in hep-2 cells. UBE2N silence significantly enhanced the proliferation of hep-2 cells. After UBE2N silencing and certain doses of radiation, the proliferation ability of hep-2 cells was enhanced, the S stage proportion was increased while, the G2 stage proportion was decreased, the apoptosis rate was decreased while the clonal formation ability was enhanced, compared with the control group (all P < 0.05). Conclusion As a tumor suppressor gene, UBE2N enhances the radiosensitivity of laryngeal squamous cell carcinoma by regulating cell proliferation, cell cycle, apoptosis and clonal formation ability.http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.1648.htmube2nlaryngeal squamous cell carcinomaradiosensitivity |
| spellingShingle | GONG Mengxiao DENG Xinzhou SHEN Li ZHANG Menglin KE Qing WU Lin LIU Yanqin CHEN Xiaolin QIU Xiaoyan LUO Zhiguo Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells Zhongliu Fangzhi Yanjiu ube2n laryngeal squamous cell carcinoma radiosensitivity |
| title | Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells |
| title_full | Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells |
| title_fullStr | Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells |
| title_full_unstemmed | Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells |
| title_short | Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells |
| title_sort | effects of ube2n knockdown on radiosensitivity of human laryngeal squamous cell carcinoma hep 2 cells |
| topic | ube2n laryngeal squamous cell carcinoma radiosensitivity |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.1648.htm |
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