Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection
Abstract Background Evaluating the serum levels of IP-10, MCP-1, MIP-1α, and IL-6 and genotyping of rs12252 SNP of IFITM3 gene among different categories of COVID-19 patients might aid in understanding the pathogenesis of COVID-19 and contribute to developing disease-specific biomarkers and therapeu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-07-01
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| Series: | The Egyptian Journal of Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43162-022-00141-9 |
| _version_ | 1811224209828872192 |
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| author | Shafia Mulla Md.Maruf Ahmed Molla S. M. Ali Ahmed A. K. M. Akhtaruzzaman Ahmed Abu Saleh Shaheda Anwar |
| author_facet | Shafia Mulla Md.Maruf Ahmed Molla S. M. Ali Ahmed A. K. M. Akhtaruzzaman Ahmed Abu Saleh Shaheda Anwar |
| author_sort | Shafia Mulla |
| collection | DOAJ |
| description | Abstract Background Evaluating the serum levels of IP-10, MCP-1, MIP-1α, and IL-6 and genotyping of rs12252 SNP of IFITM3 gene among different categories of COVID-19 patients might aid in understanding the pathogenesis of COVID-19 and contribute to developing disease-specific biomarkers and therapeutic strategies. Methods This is a cross-sectional study involving a total of 84 COVID-19 patients confirmed by positive RT-PCR and 28 healthy controls. COVID-19 patients were recruited from the intensive care unit (ICU) and COVID unit of Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka. COVID-19 patients were categorized into moderate, severe, and critically ill groups according to the World Health Organization classification. The serum IP-10, MCP-1, and MIP-1α levels were measured by cytometric bead array assay by flow cytometry, and serum IL-6 level was detected by the chemiluminescence method. rs12252 SNP of the IFITM3 gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP). Results The serum IP-10, MCP-1, MIP-1α, and IL-6 levels among critically ill COVID-19 patients were significantly higher than that in patients with moderate disease and healthy controls (p < 0.001). Genotype distribution for rs12252 (42 T/C) SNP of the IFITM3 gene between the different groups of COVID-19 patients and healthy controls showed that CC genotype was statistically associated with disease severity (p < 0.001). Conclusions IP-10 and MCP-1, MIP-α, IL-6, and CC genotype of rs12252 (42 T/C) SNP of IFITM3 gene are associated with COVID-19 severity. |
| first_indexed | 2024-04-12T08:44:35Z |
| format | Article |
| id | doaj.art-fb5cf5b352ae4848a4c8ff985f2ab3f1 |
| institution | Directory Open Access Journal |
| issn | 1110-7782 2090-9098 |
| language | English |
| last_indexed | 2024-04-12T08:44:35Z |
| publishDate | 2022-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | The Egyptian Journal of Internal Medicine |
| spelling | doaj.art-fb5cf5b352ae4848a4c8ff985f2ab3f12022-12-22T03:39:45ZengSpringerOpenThe Egyptian Journal of Internal Medicine1110-77822090-90982022-07-013411710.1186/s43162-022-00141-9Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infectionShafia Mulla0Md.Maruf Ahmed Molla1S. M. Ali Ahmed2A. K. M. Akhtaruzzaman3Ahmed Abu Saleh4Shaheda Anwar5Department of Microbiology &Immunology, Bangabandhu Sheikh Mujib Medical UniversityDepartment of Virology, National Institute of Laboratory Medicine and Referral CenterDepartment of Microbiology &Immunology, Bangabandhu Sheikh Mujib Medical UniversityDepartment of Anaesthesia, Analgesia and Intensive Care Medicine, Bangabandhu Sheikh Mujib Medical UniversityDepartment of Microbiology &Immunology, Bangabandhu Sheikh Mujib Medical UniversityDepartment of Microbiology &Immunology, Bangabandhu Sheikh Mujib Medical UniversityAbstract Background Evaluating the serum levels of IP-10, MCP-1, MIP-1α, and IL-6 and genotyping of rs12252 SNP of IFITM3 gene among different categories of COVID-19 patients might aid in understanding the pathogenesis of COVID-19 and contribute to developing disease-specific biomarkers and therapeutic strategies. Methods This is a cross-sectional study involving a total of 84 COVID-19 patients confirmed by positive RT-PCR and 28 healthy controls. COVID-19 patients were recruited from the intensive care unit (ICU) and COVID unit of Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka. COVID-19 patients were categorized into moderate, severe, and critically ill groups according to the World Health Organization classification. The serum IP-10, MCP-1, and MIP-1α levels were measured by cytometric bead array assay by flow cytometry, and serum IL-6 level was detected by the chemiluminescence method. rs12252 SNP of the IFITM3 gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP). Results The serum IP-10, MCP-1, MIP-1α, and IL-6 levels among critically ill COVID-19 patients were significantly higher than that in patients with moderate disease and healthy controls (p < 0.001). Genotype distribution for rs12252 (42 T/C) SNP of the IFITM3 gene between the different groups of COVID-19 patients and healthy controls showed that CC genotype was statistically associated with disease severity (p < 0.001). Conclusions IP-10 and MCP-1, MIP-α, IL-6, and CC genotype of rs12252 (42 T/C) SNP of IFITM3 gene are associated with COVID-19 severity.https://doi.org/10.1186/s43162-022-00141-9IP-10MCP-1MIP-1αIL-6IFITM3COVID-19 |
| spellingShingle | Shafia Mulla Md.Maruf Ahmed Molla S. M. Ali Ahmed A. K. M. Akhtaruzzaman Ahmed Abu Saleh Shaheda Anwar Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection The Egyptian Journal of Internal Medicine IP-10 MCP-1 MIP-1α IL-6 IFITM3 COVID-19 |
| title | Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection |
| title_full | Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection |
| title_fullStr | Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection |
| title_full_unstemmed | Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection |
| title_short | Association of interferon gamma inducible protein-10, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha, interleukin-6, and rs12252 single nucleotide polymorphism of interferon-induced transmembrane protein-3 gene with the severity of COVID-19 infection |
| title_sort | association of interferon gamma inducible protein 10 monocyte chemoattractant protein 1 macrophage inflammatory protein 1 alpha interleukin 6 and rs12252 single nucleotide polymorphism of interferon induced transmembrane protein 3 gene with the severity of covid 19 infection |
| topic | IP-10 MCP-1 MIP-1α IL-6 IFITM3 COVID-19 |
| url | https://doi.org/10.1186/s43162-022-00141-9 |
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