(R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism
Psoriasis is a skin disease that is characterized by a high degree of inflammation caused by immune dysfunction. (<i>R</i>)-salbutamol is a bronchodilator for asthma and was reported to alleviate immune system reactions in several diseases. In this study, using imiquimod (IMQ)-induced mo...
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MDPI AG
2020-02-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/2/511 |
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| author | Fei Liu Shanping Wang Bo Liu Yukun Wang Wen Tan |
| author_facet | Fei Liu Shanping Wang Bo Liu Yukun Wang Wen Tan |
| author_sort | Fei Liu |
| collection | DOAJ |
| description | Psoriasis is a skin disease that is characterized by a high degree of inflammation caused by immune dysfunction. (<i>R</i>)-salbutamol is a bronchodilator for asthma and was reported to alleviate immune system reactions in several diseases. In this study, using imiquimod (IMQ)-induced mouse psoriasis-like dermatitis model, we evaluated the therapeutic effects of (<i>R</i>)-salbutamol in psoriasis in vivo, and explored the metabolic pathway involved. The results showed that, compared with IMQ group, (<i>R</i>)-salbutamol treatment significantly ameliorated psoriasis, reversed the suppressive effects of IMQ on differentiation, excessive keratinocyte proliferation, and infiltration of inflammatory cells. Enzyme-linked immunosorbent assays (ELISA) showed that (<i>R</i>)-salbutamol markedly reduced the plasma levels of IL-17. Cell analysis using flow cytometry showed that (<i>R</i>)-salbutamol decreased the proportion of CD4+ Th17+ T cells (Th17), whereas it increased the percentage of CD25+ Foxp3+ regulatory T cells (Tregs) in the spleens. (<i>R</i>)-salbutamol also reduced the increased weight ratio of spleen to body. Furthermore, untargeted metabolomics showed that (<i>R</i>)-salbutamol affected three metabolic pathways, including (i) arachidonic acid metabolism, (ii) sphingolipid metabolism, and (iii) glycerophospholipid metabolism. These results demonstrated that (<i>R</i>)-salbutamol can alleviate IMQ-induced psoriasis through regulating Th17/Tregs cell response and glycerophospholipid metabolism. It may provide a new use of (<i>R</i>)-salbutamol in the management of psoriasis. |
| first_indexed | 2024-03-12T20:12:18Z |
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| last_indexed | 2024-03-12T20:12:18Z |
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| spelling | doaj.art-fb5ea408b1c74671b6904c8de3f9918a2023-08-02T01:37:17ZengMDPI AGCells2073-44092020-02-019251110.3390/cells9020511cells9020511(R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid MetabolismFei Liu0Shanping Wang1Bo Liu2Yukun Wang3Wen Tan4Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaInstitute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaInstitute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaInstitute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaInstitute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaPsoriasis is a skin disease that is characterized by a high degree of inflammation caused by immune dysfunction. (<i>R</i>)-salbutamol is a bronchodilator for asthma and was reported to alleviate immune system reactions in several diseases. In this study, using imiquimod (IMQ)-induced mouse psoriasis-like dermatitis model, we evaluated the therapeutic effects of (<i>R</i>)-salbutamol in psoriasis in vivo, and explored the metabolic pathway involved. The results showed that, compared with IMQ group, (<i>R</i>)-salbutamol treatment significantly ameliorated psoriasis, reversed the suppressive effects of IMQ on differentiation, excessive keratinocyte proliferation, and infiltration of inflammatory cells. Enzyme-linked immunosorbent assays (ELISA) showed that (<i>R</i>)-salbutamol markedly reduced the plasma levels of IL-17. Cell analysis using flow cytometry showed that (<i>R</i>)-salbutamol decreased the proportion of CD4+ Th17+ T cells (Th17), whereas it increased the percentage of CD25+ Foxp3+ regulatory T cells (Tregs) in the spleens. (<i>R</i>)-salbutamol also reduced the increased weight ratio of spleen to body. Furthermore, untargeted metabolomics showed that (<i>R</i>)-salbutamol affected three metabolic pathways, including (i) arachidonic acid metabolism, (ii) sphingolipid metabolism, and (iii) glycerophospholipid metabolism. These results demonstrated that (<i>R</i>)-salbutamol can alleviate IMQ-induced psoriasis through regulating Th17/Tregs cell response and glycerophospholipid metabolism. It may provide a new use of (<i>R</i>)-salbutamol in the management of psoriasis.https://www.mdpi.com/2073-4409/9/2/511(<i>r</i>)-salbutamolpsoriasisimmune-regulationth17/tregsmetabolomics |
| spellingShingle | Fei Liu Shanping Wang Bo Liu Yukun Wang Wen Tan (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism Cells (<i>r</i>)-salbutamol psoriasis immune-regulation th17/tregs metabolomics |
| title | (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism |
| title_full | (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism |
| title_fullStr | (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism |
| title_full_unstemmed | (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism |
| title_short | (R)-Salbutamol Improves Imiquimod-Induced Psoriasis-Like Skin Dermatitis by Regulating the Th17/Tregs Balance and Glycerophospholipid Metabolism |
| title_sort | r salbutamol improves imiquimod induced psoriasis like skin dermatitis by regulating the th17 tregs balance and glycerophospholipid metabolism |
| topic | (<i>r</i>)-salbutamol psoriasis immune-regulation th17/tregs metabolomics |
| url | https://www.mdpi.com/2073-4409/9/2/511 |
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