Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings
Effects of chilling and acclimation on the activity of cytosolic (GS1) and plastidic (GS2) isoforms of glutamine synthetase (E.C. 6.3.1.2) were studied in chilling-sensitive and acclimation-responsive maize inbred G50. Glutamine synthetase activity in mesocotyls and roots of chilled (7 d/4ºC) and re...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2007-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640703177S.pdf |
| _version_ | 1819135018294837248 |
|---|---|
| author | Simonović Ana D. Anderson M.D. |
| author_facet | Simonović Ana D. Anderson M.D. |
| author_sort | Simonović Ana D. |
| collection | DOAJ |
| description | Effects of chilling and acclimation on the activity of cytosolic (GS1) and plastidic (GS2) isoforms of glutamine synthetase (E.C. 6.3.1.2) were studied in chilling-sensitive and acclimation-responsive maize inbred G50. Glutamine synthetase activity in mesocotyls and roots of chilled (7 d/4ºC) and rewarmed (1 d/27ºC) etiolated plants was "1/3 that of controls. In coleoptiles+leaves of light-grown plants, GS1 was reduced to 75%, and GS2 to 50%. Acclimation (3 d/14ºC) increased GS activity and alleviated the effects of chilling. Exposure to H2O2 or menadione also reduced GS activity. Since chilling causes oxidative stress in maize, acclimation probably preserves GS activity by protecting GS from oxidative inactivation. . |
| first_indexed | 2024-12-22T10:12:25Z |
| format | Article |
| id | doaj.art-fb5fd08f72b641d7b0eedbf23d914b88 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-12-22T10:12:25Z |
| publishDate | 2007-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-fb5fd08f72b641d7b0eedbf23d914b882022-12-21T18:29:50ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642007-01-0159317718510.2298/ABS0703177SEffect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlingsSimonović Ana D.Anderson M.D.Effects of chilling and acclimation on the activity of cytosolic (GS1) and plastidic (GS2) isoforms of glutamine synthetase (E.C. 6.3.1.2) were studied in chilling-sensitive and acclimation-responsive maize inbred G50. Glutamine synthetase activity in mesocotyls and roots of chilled (7 d/4ºC) and rewarmed (1 d/27ºC) etiolated plants was "1/3 that of controls. In coleoptiles+leaves of light-grown plants, GS1 was reduced to 75%, and GS2 to 50%. Acclimation (3 d/14ºC) increased GS activity and alleviated the effects of chilling. Exposure to H2O2 or menadione also reduced GS activity. Since chilling causes oxidative stress in maize, acclimation probably preserves GS activity by protecting GS from oxidative inactivation. .http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640703177S.pdfacclimationchillingglutamine synthetasemaizeoxidative stressphosphate precipitation |
| spellingShingle | Simonović Ana D. Anderson M.D. Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings Archives of Biological Sciences acclimation chilling glutamine synthetase maize oxidative stress phosphate precipitation |
| title | Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| title_full | Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| title_fullStr | Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| title_full_unstemmed | Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| title_short | Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| title_sort | effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings |
| topic | acclimation chilling glutamine synthetase maize oxidative stress phosphate precipitation |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640703177S.pdf |
| work_keys_str_mv | AT simonovicanad effectofchillingandacclimationontheactivityofglutaminesynthetaseisoformsinmaizeseedlings AT andersonmd effectofchillingandacclimationontheactivityofglutaminesynthetaseisoformsinmaizeseedlings |