Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues
Background: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT). ...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Biochemistry and Molecular Biology
2019-12-01
|
| Series: | Acta Biochimica Indonesiana |
| Subjects: | |
| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/44 |
| _version_ | 1818948807355793408 |
|---|---|
| author | Rizka Ramadhani Ani Retno Prijanti |
| author_facet | Rizka Ramadhani Ani Retno Prijanti |
| author_sort | Rizka Ramadhani |
| collection | DOAJ |
| description | Background: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT).
Objective: To investigate whether the specific activities of alanine aminotransferase (ALT) increased in hypoxic rat brain.
Methods: This experimental study used rats exposed to systemic normobaric hypoxia during 14 days. A group of 5 rats was sacrificed in days 1, 3, 7 and 14. The specific activities of ALT were analyzed in their brains using a reaction coupled with lactate dehydrogenase (LDH) activities.
Results: The ALT specific activities in rat brain were very low. There was no significant increase of specific activities during long term hypoxia (p > 0.05).
Conclusion: The rat brain ALT has no role in gluconeogenesis. |
| first_indexed | 2024-12-20T08:52:40Z |
| format | Article |
| id | doaj.art-fb63b7ff820141a988f3f99aba04821d |
| institution | Directory Open Access Journal |
| issn | 2654-6108 2654-3222 |
| language | English |
| last_indexed | 2024-12-20T08:52:40Z |
| publishDate | 2019-12-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj.art-fb63b7ff820141a988f3f99aba04821d2022-12-21T19:46:05ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222019-12-012210.32889/actabioina.v2i2.43Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissuesRizka Ramadhani0Ani Retno Prijanti1Faculty of Medicine, Universitas Indonesia, Jakarta, IndonesiaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, IndonesiaBackground: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT). Objective: To investigate whether the specific activities of alanine aminotransferase (ALT) increased in hypoxic rat brain. Methods: This experimental study used rats exposed to systemic normobaric hypoxia during 14 days. A group of 5 rats was sacrificed in days 1, 3, 7 and 14. The specific activities of ALT were analyzed in their brains using a reaction coupled with lactate dehydrogenase (LDH) activities. Results: The ALT specific activities in rat brain were very low. There was no significant increase of specific activities during long term hypoxia (p > 0.05). Conclusion: The rat brain ALT has no role in gluconeogenesis.https://pbbmi.org/newjurnal/index.php/actabioina/article/view/44ALTBrainGluconeogenesisHypoxia |
| spellingShingle | Rizka Ramadhani Ani Retno Prijanti Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues Acta Biochimica Indonesiana ALT Brain Gluconeogenesis Hypoxia |
| title | Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues |
| title_full | Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues |
| title_fullStr | Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues |
| title_full_unstemmed | Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues |
| title_short | Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues |
| title_sort | alanine amino transferase alt specific activities in long term systemic hypoxic rat brain tissues |
| topic | ALT Brain Gluconeogenesis Hypoxia |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/44 |
| work_keys_str_mv | AT rizkaramadhani alanineaminotransferasealtspecificactivitiesinlongtermsystemichypoxicratbraintissues AT aniretnoprijanti alanineaminotransferasealtspecificactivitiesinlongtermsystemichypoxicratbraintissues |