Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2
ABSTRACT Porcine circovirus type 2 (PCV2) is an important swine infectious pathogen that seriously threatens the global swine industry. PCV2 Cap protein is the only structural and the main immunogenic protein constituting the viral capsid. In this study, a gold nanoparticle-based immunochromatograph...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01953-22 |
| _version_ | 1797741733974376448 |
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| author | Min Jiang Aiping Wang Yaning Sun Yuan Li Yumei Chen Jingming Zhou Hongliang Liu Peiyang Ding Yanhua Qi Ning Li Gaiping Zhang |
| author_facet | Min Jiang Aiping Wang Yaning Sun Yuan Li Yumei Chen Jingming Zhou Hongliang Liu Peiyang Ding Yanhua Qi Ning Li Gaiping Zhang |
| author_sort | Min Jiang |
| collection | DOAJ |
| description | ABSTRACT Porcine circovirus type 2 (PCV2) is an important swine infectious pathogen that seriously threatens the global swine industry. PCV2 Cap protein is the only structural and the main immunogenic protein constituting the viral capsid. In this study, a gold nanoparticle-based immunochromatographic strip with high sensitivity and specificity was developed which could be used for rapid detection of PCV2 virions or Cap protein in research. The visual detection limit of the strip was 103.18 50% tissue culture infective does (TCID50)/mL for PCV2, and 2.03 μg/mL for PCV2 Cap protein. No cross-reactivity was observed with the PCV1 and PCV3 Cap proteins and other common swine pathogens such as porcine reproductive and respiratory syndrome virus, classical swine fever virus, pseudorabies virus, porcine epidemic diarrhea virus, porcine parvovirus, and swine influenza virus. The repeatability of the strip was good. The stability of the strip was perfect for 12 months in a dry state at room temperature. Visual results could be obtained within 5 min by simply inserting the strip into the diluted sample. The strip is a time-saving, labor-saving, and reliable tool for testing of PCV2 virions or Cap protein in research. The idea of this study might open a new perspective for the application of the strip. IMPORTANCE Porcine circovirus type 2 (PCV2) Cap protein is the only structural and the main immunogenic protein constituting the viral capsid. Although many methods can be used to identify PCV2 or PCV2 Cap protein in vaccine research, they usually require high workload and time. The developed strip can specifically detect PCV2 virions or Cap protein, and visual qualitative results can be obtained within 5 min by simply diluting the sample and inserting the strip into the sample. The final value of the strip is providing a simple and time-saving method for real-time monitoring of PCV2 antigen in vaccine research with reliable results, such as the different stages of PCV2 Cap protein expression and purification, as well as the different stages of PCV2 reproduction and purification. |
| first_indexed | 2024-03-12T14:30:55Z |
| format | Article |
| id | doaj.art-fb6636fb614143bfab21a264ffecf57c |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-12T14:30:55Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-fb6636fb614143bfab21a264ffecf57c2023-08-17T13:04:15ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-08-0111410.1128/spectrum.01953-22Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2Min Jiang0Aiping Wang1Yaning Sun2Yuan Li3Yumei Chen4Jingming Zhou5Hongliang Liu6Peiyang Ding7Yanhua Qi8Ning Li9Gaiping Zhang10Longhu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaSchool of Life Sciences, Lanzhou University, Lanzhou, Gansu, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaLonghu Laboratory of Advanced Immunology, Zhengzhou, Henan, ChinaABSTRACT Porcine circovirus type 2 (PCV2) is an important swine infectious pathogen that seriously threatens the global swine industry. PCV2 Cap protein is the only structural and the main immunogenic protein constituting the viral capsid. In this study, a gold nanoparticle-based immunochromatographic strip with high sensitivity and specificity was developed which could be used for rapid detection of PCV2 virions or Cap protein in research. The visual detection limit of the strip was 103.18 50% tissue culture infective does (TCID50)/mL for PCV2, and 2.03 μg/mL for PCV2 Cap protein. No cross-reactivity was observed with the PCV1 and PCV3 Cap proteins and other common swine pathogens such as porcine reproductive and respiratory syndrome virus, classical swine fever virus, pseudorabies virus, porcine epidemic diarrhea virus, porcine parvovirus, and swine influenza virus. The repeatability of the strip was good. The stability of the strip was perfect for 12 months in a dry state at room temperature. Visual results could be obtained within 5 min by simply inserting the strip into the diluted sample. The strip is a time-saving, labor-saving, and reliable tool for testing of PCV2 virions or Cap protein in research. The idea of this study might open a new perspective for the application of the strip. IMPORTANCE Porcine circovirus type 2 (PCV2) Cap protein is the only structural and the main immunogenic protein constituting the viral capsid. Although many methods can be used to identify PCV2 or PCV2 Cap protein in vaccine research, they usually require high workload and time. The developed strip can specifically detect PCV2 virions or Cap protein, and visual qualitative results can be obtained within 5 min by simply diluting the sample and inserting the strip into the sample. The final value of the strip is providing a simple and time-saving method for real-time monitoring of PCV2 antigen in vaccine research with reliable results, such as the different stages of PCV2 Cap protein expression and purification, as well as the different stages of PCV2 reproduction and purification.https://journals.asm.org/doi/10.1128/spectrum.01953-22porcine circovirus type 2monoclonal antibodyrapid detectionimmunochromatographic stripcapsid protein |
| spellingShingle | Min Jiang Aiping Wang Yaning Sun Yuan Li Yumei Chen Jingming Zhou Hongliang Liu Peiyang Ding Yanhua Qi Ning Li Gaiping Zhang Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 Microbiology Spectrum porcine circovirus type 2 monoclonal antibody rapid detection immunochromatographic strip capsid protein |
| title | Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 |
| title_full | Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 |
| title_fullStr | Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 |
| title_full_unstemmed | Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 |
| title_short | Development of a Gold Nanoparticle-Based Immunochromatographic Strip for Rapid Detection of Porcine Circovirus Type 2 |
| title_sort | development of a gold nanoparticle based immunochromatographic strip for rapid detection of porcine circovirus type 2 |
| topic | porcine circovirus type 2 monoclonal antibody rapid detection immunochromatographic strip capsid protein |
| url | https://journals.asm.org/doi/10.1128/spectrum.01953-22 |
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