Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia
Sequencing of cloned PCR-amplified species-specific rDNA fragments and isolation of axenic cultures from tomato fruits was carried out to study the mycobiota of tomato leaves and fruits in European part of Russia. DNA was extracted from the leaves, and library of ITS region fragments was constructed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Bulgarian |
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University of Zagreb, Faculty of Agriculture
2020-12-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | https://jcea.agr.hr/articles/772938_Fungal_diversity_in_tomato_(Solanum_lycopersicum)_leaves_and_fruits_in_Russia_en.pdf |
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| author | Lyudmila Kokaeva Elena Chudinova Alexander Berezov Maria Yarmeeva Peotr Balabko Arseniy Belosokhov Sergey Elansky |
| author_facet | Lyudmila Kokaeva Elena Chudinova Alexander Berezov Maria Yarmeeva Peotr Balabko Arseniy Belosokhov Sergey Elansky |
| author_sort | Lyudmila Kokaeva |
| collection | DOAJ |
| description | Sequencing of cloned PCR-amplified species-specific rDNA fragments and isolation of axenic cultures from tomato fruits was carried out to study the mycobiota of tomato leaves and fruits in European part of Russia. DNA was extracted from the leaves, and library of ITS region fragments was constructed in E. coli by cloning of PCR products. This survey revealed fourteen species associated with disease-affected leaves: Septoria lycopersici, Fulvia fulva (=Cladosporium fulvum), Didymella glomerata (=Phoma glomerata), Cladosporium herbarum, Podosphaera fusca, Neocamarosporium goegapense (=Phoma betae), Rhizoctonia solani, Candida albicans, Dioszegia hungarica, Cladosporium cladosporioides, Didymella lycopersici, Alternaria infectoria, Alternaria alternata, Cryptococcus tephrensis. In the leaves from healthy plants without any visible symptoms DNA of three species was found: Aspergillus versicolor, Alternaria alternata, Aureobasidium pullulans. Analysis of axenic cultures isolated from green diseased tomato fruits revealed fungal species: Alternaria alternata, Alternaria solani, Phomopsis phaseoli, Fusarium equiseti, Chaetomium cochliodes, Clonostachys sp., Irpex lacteus, Colletotrichum coccodes. This research provides new information on the mycobiota of tomato in Southern Russia, the main tomato producing region of the country. |
| first_indexed | 2024-12-14T03:55:36Z |
| format | Article |
| id | doaj.art-fb6b3fe0cdca4786a6f9e6c3080ef3b7 |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-12-14T03:55:36Z |
| publishDate | 2020-12-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-fb6b3fe0cdca4786a6f9e6c3080ef3b72022-12-21T23:18:06ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492020-12-0121480981610.5513/JCEA01/21.4.2869Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in RussiaLyudmila KokaevaElena ChudinovaAlexander BerezovMaria YarmeevaPeotr BalabkoArseniy BelosokhovSergey ElanskySequencing of cloned PCR-amplified species-specific rDNA fragments and isolation of axenic cultures from tomato fruits was carried out to study the mycobiota of tomato leaves and fruits in European part of Russia. DNA was extracted from the leaves, and library of ITS region fragments was constructed in E. coli by cloning of PCR products. This survey revealed fourteen species associated with disease-affected leaves: Septoria lycopersici, Fulvia fulva (=Cladosporium fulvum), Didymella glomerata (=Phoma glomerata), Cladosporium herbarum, Podosphaera fusca, Neocamarosporium goegapense (=Phoma betae), Rhizoctonia solani, Candida albicans, Dioszegia hungarica, Cladosporium cladosporioides, Didymella lycopersici, Alternaria infectoria, Alternaria alternata, Cryptococcus tephrensis. In the leaves from healthy plants without any visible symptoms DNA of three species was found: Aspergillus versicolor, Alternaria alternata, Aureobasidium pullulans. Analysis of axenic cultures isolated from green diseased tomato fruits revealed fungal species: Alternaria alternata, Alternaria solani, Phomopsis phaseoli, Fusarium equiseti, Chaetomium cochliodes, Clonostachys sp., Irpex lacteus, Colletotrichum coccodes. This research provides new information on the mycobiota of tomato in Southern Russia, the main tomato producing region of the country.https://jcea.agr.hr/articles/772938_Fungal_diversity_in_tomato_(Solanum_lycopersicum)_leaves_and_fruits_in_Russia_en.pdfmycobiota of tomato leavesphylloplaneplant pathogenic fungifungal species identificationtomato diseasesfungal diversity |
| spellingShingle | Lyudmila Kokaeva Elena Chudinova Alexander Berezov Maria Yarmeeva Peotr Balabko Arseniy Belosokhov Sergey Elansky Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia Journal of Central European Agriculture mycobiota of tomato leaves phylloplane plant pathogenic fungi fungal species identification tomato diseases fungal diversity |
| title | Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia |
| title_full | Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia |
| title_fullStr | Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia |
| title_full_unstemmed | Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia |
| title_short | Fungal diversity in tomato (Solanum lycopersicum) leaves and fruits in Russia |
| title_sort | fungal diversity in tomato solanum lycopersicum leaves and fruits in russia |
| topic | mycobiota of tomato leaves phylloplane plant pathogenic fungi fungal species identification tomato diseases fungal diversity |
| url | https://jcea.agr.hr/articles/772938_Fungal_diversity_in_tomato_(Solanum_lycopersicum)_leaves_and_fruits_in_Russia_en.pdf |
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