Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula
Fucan is a term used to denominate a type of polysaccharide which contains substantial percentages of L-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed mai...
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| Format: | Article |
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MDPI AG
2011-06-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/9/6/952/ |
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| author | Hugo Alexandre Oliveira Rocha Giulianna Paiva Viana Andrade Ivan Rui Lopes Albuquerque Ruth Medeiros Oliveira Raniere Fagundes Melo-Silveira Jailma Almeida-Lima Sara Lima Cordeiro Mariana Santana Santos Pereira Costa Rafael Barros Gomes Camara Nednaldo Dantas-Santos Gabriel Pereira Fidelis Cinthia Beatrice Silva Telles Leandro Silva Costa |
| author_facet | Hugo Alexandre Oliveira Rocha Giulianna Paiva Viana Andrade Ivan Rui Lopes Albuquerque Ruth Medeiros Oliveira Raniere Fagundes Melo-Silveira Jailma Almeida-Lima Sara Lima Cordeiro Mariana Santana Santos Pereira Costa Rafael Barros Gomes Camara Nednaldo Dantas-Santos Gabriel Pereira Fidelis Cinthia Beatrice Silva Telles Leandro Silva Costa |
| author_sort | Hugo Alexandre Oliveira Rocha |
| collection | DOAJ |
| description | Fucan is a term used to denominate a type of polysaccharide which contains substantial percentages of L-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed mainly of fucose, glucose, glucuronic acid, galactose and sulfate. These fucans did not show anticoagulant activity in PT and aPTT tests. Their antioxidant activity was evaluated using the follow tests; total antioxidant capacity, scavenging hydroxyl and superoxide radicals, reducing power and ferrous ion [Fe(II)] chelating. All heterofucans displayed considerable activity, especially SF-1.0v which showed the most significant antioxidant potential with 90.7 ascorbic acid equivalents in a total antioxidant capacity test and similar activity when compared with vitamin C in a reducing power assay. The fucan antiproliferative activity was performed with HeLa, PC3 and HepG2 cells using MTT test. In all tested conditions the heterofucans exhibited a dose-dependent effect. The strongest inhibition was observed in HeLa cells, where SF-1.0 and SF-1.5 exhibited considerable activity with an IC50 value of 15.69 and 13.83 µM, respectively. These results clearly indicate the beneficial effect of S. filipendula polysaccharides as antiproliferative and antioxidant. Further purification steps and additional studies on structural features as well as in vivo experiments are needed to test the viability of their use as therapeutic agents. |
| first_indexed | 2024-04-11T18:00:23Z |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:00:23Z |
| publishDate | 2011-06-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj.art-fb6b646ac1294b9c8ea8b277728913d62022-12-22T04:10:32ZengMDPI AGMarine Drugs1660-33972011-06-019695296610.3390/md9060952Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendulaHugo Alexandre Oliveira RochaGiulianna Paiva Viana AndradeIvan Rui Lopes AlbuquerqueRuth Medeiros OliveiraRaniere Fagundes Melo-SilveiraJailma Almeida-LimaSara Lima CordeiroMariana Santana Santos Pereira CostaRafael Barros Gomes CamaraNednaldo Dantas-SantosGabriel Pereira FidelisCinthia Beatrice Silva TellesLeandro Silva CostaFucan is a term used to denominate a type of polysaccharide which contains substantial percentages of L-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed mainly of fucose, glucose, glucuronic acid, galactose and sulfate. These fucans did not show anticoagulant activity in PT and aPTT tests. Their antioxidant activity was evaluated using the follow tests; total antioxidant capacity, scavenging hydroxyl and superoxide radicals, reducing power and ferrous ion [Fe(II)] chelating. All heterofucans displayed considerable activity, especially SF-1.0v which showed the most significant antioxidant potential with 90.7 ascorbic acid equivalents in a total antioxidant capacity test and similar activity when compared with vitamin C in a reducing power assay. The fucan antiproliferative activity was performed with HeLa, PC3 and HepG2 cells using MTT test. In all tested conditions the heterofucans exhibited a dose-dependent effect. The strongest inhibition was observed in HeLa cells, where SF-1.0 and SF-1.5 exhibited considerable activity with an IC50 value of 15.69 and 13.83 µM, respectively. These results clearly indicate the beneficial effect of S. filipendula polysaccharides as antiproliferative and antioxidant. Further purification steps and additional studies on structural features as well as in vivo experiments are needed to test the viability of their use as therapeutic agents.http://www.mdpi.com/1660-3397/9/6/952/fucoidanbiological activitiesHeLa cellsbrown seaweed |
| spellingShingle | Hugo Alexandre Oliveira Rocha Giulianna Paiva Viana Andrade Ivan Rui Lopes Albuquerque Ruth Medeiros Oliveira Raniere Fagundes Melo-Silveira Jailma Almeida-Lima Sara Lima Cordeiro Mariana Santana Santos Pereira Costa Rafael Barros Gomes Camara Nednaldo Dantas-Santos Gabriel Pereira Fidelis Cinthia Beatrice Silva Telles Leandro Silva Costa Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula Marine Drugs fucoidan biological activities HeLa cells brown seaweed |
| title | Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula |
| title_full | Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula |
| title_fullStr | Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula |
| title_full_unstemmed | Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula |
| title_short | Antioxidant and Antiproliferative Activities of Heterofucans from the Seaweed Sargassum filipendula |
| title_sort | antioxidant and antiproliferative activities of heterofucans from the seaweed sargassum filipendula |
| topic | fucoidan biological activities HeLa cells brown seaweed |
| url | http://www.mdpi.com/1660-3397/9/6/952/ |
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