A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory
A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection. The gold standard assay is the conventional plaque reduction neutralization test (PRNT) which r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/6/1211 |
| _version_ | 1797481454725234688 |
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| author | Jing Zou Hongjie Xia Pei-Yong Shi Xuping Xie Ping Ren |
| author_facet | Jing Zou Hongjie Xia Pei-Yong Shi Xuping Xie Ping Ren |
| author_sort | Jing Zou |
| collection | DOAJ |
| description | A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection. The gold standard assay is the conventional plaque reduction neutralization test (PRNT) which requires extensive labor, live viruses, and BSL-3 facilities. Recently, we developed a novel single-round infection fluorescent SARS-CoV-2 virus (SFV) that can be safely used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. In this study, we evaluated the performance of the neutralization test using this SFV with 80 PRNT-positive and 92 PRNT-negative clinical serum or plasma specimens. The SFV neutralization test (SFVNT) has 100% sensitivity and specificity compared to the PRNT. Furthermore, the neutralizing titers generated by the SFVNT and PRNT are highly correlated, with R<sup>2</sup> = 0.903 (<i>p</i> < 0.0001). Due to high sensitivity, specificity, accuracy, and reproducibility, the SFVNT can be deployed for the large-scale testing of COVID-19 patients or vaccinated people in general lab settings. |
| first_indexed | 2024-03-09T22:14:50Z |
| format | Article |
| id | doaj.art-fb6d287531484622993700a6860bcbfd |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:14:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fb6d287531484622993700a6860bcbfd2023-11-23T19:25:30ZengMDPI AGViruses1999-49152022-06-01146121110.3390/v14061211A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 LaboratoryJing Zou0Hongjie Xia1Pei-Yong Shi2Xuping Xie3Ping Ren4Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USAInstitute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX 77555, USAA robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection. The gold standard assay is the conventional plaque reduction neutralization test (PRNT) which requires extensive labor, live viruses, and BSL-3 facilities. Recently, we developed a novel single-round infection fluorescent SARS-CoV-2 virus (SFV) that can be safely used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. In this study, we evaluated the performance of the neutralization test using this SFV with 80 PRNT-positive and 92 PRNT-negative clinical serum or plasma specimens. The SFV neutralization test (SFVNT) has 100% sensitivity and specificity compared to the PRNT. Furthermore, the neutralizing titers generated by the SFVNT and PRNT are highly correlated, with R<sup>2</sup> = 0.903 (<i>p</i> < 0.0001). Due to high sensitivity, specificity, accuracy, and reproducibility, the SFVNT can be deployed for the large-scale testing of COVID-19 patients or vaccinated people in general lab settings.https://www.mdpi.com/1999-4915/14/6/1211single-round infection fluorescent SARS-CoV-2 virusneutralization assaysensitivityspecificityaccuracylinearity |
| spellingShingle | Jing Zou Hongjie Xia Pei-Yong Shi Xuping Xie Ping Ren A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory Viruses single-round infection fluorescent SARS-CoV-2 virus neutralization assay sensitivity specificity accuracy linearity |
| title | A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory |
| title_full | A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory |
| title_fullStr | A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory |
| title_full_unstemmed | A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory |
| title_short | A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory |
| title_sort | single round infection fluorescent sars cov 2 neutralization test for covid 19 serological testing at a biosafety level 2 laboratory |
| topic | single-round infection fluorescent SARS-CoV-2 virus neutralization assay sensitivity specificity accuracy linearity |
| url | https://www.mdpi.com/1999-4915/14/6/1211 |
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