Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study
Abstract Background Circulating pro-inflammatory cytokines were associated with increased relative mortality risk, while immune parameters reflecting improved T-cell function were predictors of survival in hemodialysis (HD) patients. We evaluated in the prospective study whether variants in T helper...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12882-017-0582-x |
| _version_ | 1811325570690056192 |
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| author | Alicja E. Grzegorzewska Monika K. Świderska Adrianna Mostowska Wojciech Warchoł Paweł P. Jagodziński |
| author_facet | Alicja E. Grzegorzewska Monika K. Świderska Adrianna Mostowska Wojciech Warchoł Paweł P. Jagodziński |
| author_sort | Alicja E. Grzegorzewska |
| collection | DOAJ |
| description | Abstract Background Circulating pro-inflammatory cytokines were associated with increased relative mortality risk, while immune parameters reflecting improved T-cell function were predictors of survival in hemodialysis (HD) patients. We evaluated in the prospective study whether variants in T helper cell cytokine-associated genes are determinants of mortality in HD patients. Methods The study was carried out in 532 prevalent HD subjects who were followed-up for 7 years. HRM analysis was used for IFNL3, IL12A, IL13, and IL4R genotyping. CCL2, IL12B, and IL18 were genotyped using PCR–RFLP analysis. Survival analyses were conducted using the Kaplan-Meier method and the Cox proportional hazard model. Results In univariate analyses, IFNL3 rs8099917 was associated with all-cause mortality in recessive model of inheritance (log-rank test P = 0.044), IL12A rs568408 - in dominant model (log-rank test P = 0.029). Minor homozygotes (the genotype GG) in IFNL3 rs8099917 showed shorter survival during the study (3.6, 1.0–7.0 years vs 4.7, 0.1–7.0 years, P = 0.009) than the major allele (T) bearers. The rs8099917 GG patients demonstrated higher risk of death than the remaining patients (GT + TT) (OR 1.94, 95%CI 1.11–3.40, P = 0.020). Major homozygosity (the genotype GG) in IL12A rs568408 was associated with higher mortality than that shown in bearers of the minor allele (AA + AG) (HR 1.31, 95%CI 1.02–1.69, P = 0.035). In multivariate analyses, however, the mentioned polymorphisms were not independent predictors of survival. Conclusions Polymorphisms of IFNL3 rs8099917 and IL12A rs568408 contribute to survival of HD patients, but not as independent factors. |
| first_indexed | 2024-04-13T14:35:33Z |
| format | Article |
| id | doaj.art-fb6d79bcb45f4b71aaca52faaaa6d078 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-04-13T14:35:33Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-fb6d79bcb45f4b71aaca52faaaa6d0782022-12-22T02:43:03ZengBMCBMC Nephrology1471-23692017-05-011811710.1186/s12882-017-0582-xPolymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective studyAlicja E. Grzegorzewska0Monika K. Świderska1Adrianna Mostowska2Wojciech Warchoł3Paweł P. Jagodziński4Chair and Department of Nephrology, Transplantology and Internal Diseases, Poznan University of Medical SciencesStudent Nephrology Research Group, Chair and Department of Nephrology, Transplantology and Internal Diseases, Poznan University of Medical SciencesChair and Department of Biochemistry and Molecular Biology, Poznan University of Medical SciencesChair and Department of Biophysics, Poznan University of Medical SciencesChair and Department of Biochemistry and Molecular Biology, Poznan University of Medical SciencesAbstract Background Circulating pro-inflammatory cytokines were associated with increased relative mortality risk, while immune parameters reflecting improved T-cell function were predictors of survival in hemodialysis (HD) patients. We evaluated in the prospective study whether variants in T helper cell cytokine-associated genes are determinants of mortality in HD patients. Methods The study was carried out in 532 prevalent HD subjects who were followed-up for 7 years. HRM analysis was used for IFNL3, IL12A, IL13, and IL4R genotyping. CCL2, IL12B, and IL18 were genotyped using PCR–RFLP analysis. Survival analyses were conducted using the Kaplan-Meier method and the Cox proportional hazard model. Results In univariate analyses, IFNL3 rs8099917 was associated with all-cause mortality in recessive model of inheritance (log-rank test P = 0.044), IL12A rs568408 - in dominant model (log-rank test P = 0.029). Minor homozygotes (the genotype GG) in IFNL3 rs8099917 showed shorter survival during the study (3.6, 1.0–7.0 years vs 4.7, 0.1–7.0 years, P = 0.009) than the major allele (T) bearers. The rs8099917 GG patients demonstrated higher risk of death than the remaining patients (GT + TT) (OR 1.94, 95%CI 1.11–3.40, P = 0.020). Major homozygosity (the genotype GG) in IL12A rs568408 was associated with higher mortality than that shown in bearers of the minor allele (AA + AG) (HR 1.31, 95%CI 1.02–1.69, P = 0.035). In multivariate analyses, however, the mentioned polymorphisms were not independent predictors of survival. Conclusions Polymorphisms of IFNL3 rs8099917 and IL12A rs568408 contribute to survival of HD patients, but not as independent factors.http://link.springer.com/article/10.1186/s12882-017-0582-xCytokinesGenesHemodialysisInterferon λ3SurvivalT helper cells |
| spellingShingle | Alicja E. Grzegorzewska Monika K. Świderska Adrianna Mostowska Wojciech Warchoł Paweł P. Jagodziński Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study BMC Nephrology Cytokines Genes Hemodialysis Interferon λ3 Survival T helper cells |
| title | Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study |
| title_full | Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study |
| title_fullStr | Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study |
| title_full_unstemmed | Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study |
| title_short | Polymorphisms of T helper cell cytokine-associated genes and survival of hemodialysis patients – a prospective study |
| title_sort | polymorphisms of t helper cell cytokine associated genes and survival of hemodialysis patients a prospective study |
| topic | Cytokines Genes Hemodialysis Interferon λ3 Survival T helper cells |
| url | http://link.springer.com/article/10.1186/s12882-017-0582-x |
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