Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles
This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often emplo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1194292/full |
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| author | Siti Nur Amalina Mohamad Sukri Kamyar Shameli Sin-Yeang Teow Jactty Chew Li-Ting Ooi Michiele Lee-Kiun Soon Nur Afini Ismail Hassan Moeini |
| author_facet | Siti Nur Amalina Mohamad Sukri Kamyar Shameli Sin-Yeang Teow Jactty Chew Li-Ting Ooi Michiele Lee-Kiun Soon Nur Afini Ismail Hassan Moeini |
| author_sort | Siti Nur Amalina Mohamad Sukri |
| collection | DOAJ |
| description | This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often employ toxic or hazardous chemicals. Antibacterial and anti-cancer activities of the green synthesized nanoparticles were then assessed in vitro. X-ray diffraction confirmed the production of ZnO-Ag-NPs with increasing crystallinity in higher pH values. The ZnO-Ag-NPs were found to be agglomerated with spherical Ag-NPs. Fourier Transform Infrared (FTIR) spectra revealed a broad band in ZnO-Ag-NPs ranging from 400−1 to 530 cm−1 with reduced intensity as compared to ZnO-NPs, indicating the formation of Ag-NPs on the surface of ZnO-NPs. The synthesized ZnO-Ag-NPs exhibited potent antibacterial activity against a broad spectrum of bacterial strains, particularly Gram-positive bacteria, with superior inhibition activity compared to ZnO-NPs. Moreover, ZnO-Ag-NPs showed a dose-dependent anti-proliferative effect on colorectal-, lung-, and cervical cancer cells. ZnO-Ag-NPs showed significantly greater efficacy in inhibiting cancer cell growth at a lower concentration of 31.25 μg/mL, compared to ZnO-NPs which required over 500 μg/mL, possibly due to the presence of silver nanoparticles (Ag-NPs). The results obtained from this study demonstrate the potential of green synthesis approaches in the fabrication of therapeutic nanomaterials for cancer treatment, as well as other biomedical applications. |
| first_indexed | 2024-03-12T21:21:19Z |
| format | Article |
| id | doaj.art-fb6dd8723968489a8860191a298113fa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T21:21:19Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fb6dd8723968489a8860191a298113fa2023-07-28T20:37:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.11942921194292Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticlesSiti Nur Amalina Mohamad Sukri0Kamyar Shameli1Sin-Yeang Teow2Jactty Chew3Li-Ting Ooi4Michiele Lee-Kiun Soon5Nur Afini Ismail6Hassan Moeini7Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, MalaysiaSchool of Medicine, Institute of Virology, Technical University of Munich, Munich, GermanyDepartment of Biology, College of Science, Mathematics and Technology, Wenzhou-Kean University, Wenzhou, Zhejiang, ChinaDepartment of Biological Sciences, School of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, MalaysiaSchool of Health Sciences, International Medical University, Kuala Lumpur, MalaysiaDepartment of Medical Sciences, School of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, MalaysiaMalaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, MalaysiaSchool of Medicine, Institute of Virology, Technical University of Munich, Munich, GermanyThis study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often employ toxic or hazardous chemicals. Antibacterial and anti-cancer activities of the green synthesized nanoparticles were then assessed in vitro. X-ray diffraction confirmed the production of ZnO-Ag-NPs with increasing crystallinity in higher pH values. The ZnO-Ag-NPs were found to be agglomerated with spherical Ag-NPs. Fourier Transform Infrared (FTIR) spectra revealed a broad band in ZnO-Ag-NPs ranging from 400−1 to 530 cm−1 with reduced intensity as compared to ZnO-NPs, indicating the formation of Ag-NPs on the surface of ZnO-NPs. The synthesized ZnO-Ag-NPs exhibited potent antibacterial activity against a broad spectrum of bacterial strains, particularly Gram-positive bacteria, with superior inhibition activity compared to ZnO-NPs. Moreover, ZnO-Ag-NPs showed a dose-dependent anti-proliferative effect on colorectal-, lung-, and cervical cancer cells. ZnO-Ag-NPs showed significantly greater efficacy in inhibiting cancer cell growth at a lower concentration of 31.25 μg/mL, compared to ZnO-NPs which required over 500 μg/mL, possibly due to the presence of silver nanoparticles (Ag-NPs). The results obtained from this study demonstrate the potential of green synthesis approaches in the fabrication of therapeutic nanomaterials for cancer treatment, as well as other biomedical applications.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1194292/fullzinc oxidesilvernanoparticlesgreen synthesisPunica granatumantibacterial |
| spellingShingle | Siti Nur Amalina Mohamad Sukri Kamyar Shameli Sin-Yeang Teow Jactty Chew Li-Ting Ooi Michiele Lee-Kiun Soon Nur Afini Ismail Hassan Moeini Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles Frontiers in Microbiology zinc oxide silver nanoparticles green synthesis Punica granatum antibacterial |
| title | Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
| title_full | Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
| title_fullStr | Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
| title_full_unstemmed | Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
| title_short | Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
| title_sort | enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide silver nanoparticles |
| topic | zinc oxide silver nanoparticles green synthesis Punica granatum antibacterial |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1194292/full |
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