Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells
Brazilein is a compound obtained in a large amount from the dried heartwood of Secang (Caesalpinia sappan L.). Brazilein has strong cytotoxic effect in several cancer cell lines. This research was designed to evaluate the cytotoxic effect of brazilein and its combination with a chemotherapy agent, d...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2015-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/86 |
| _version_ | 1811231770599751680 |
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| author | Ni Putu Linda Laksmiani Ratna Asmah Susidarti Edy Meiyanto |
| author_facet | Ni Putu Linda Laksmiani Ratna Asmah Susidarti Edy Meiyanto |
| author_sort | Ni Putu Linda Laksmiani |
| collection | DOAJ |
| description | Brazilein is a compound obtained in a large amount from the dried heartwood of Secang (Caesalpinia sappan L.). Brazilein has strong cytotoxic effect in several cancer cell lines. This research was designed to evaluate the cytotoxic effect of brazilein and its combination with a chemotherapy agent, doxorubicin on MCF-7/DOX breast cancer cells. In the cytotoxicity assay, MCF-7/DOX cells were cultured in the presence of brazilein solely and in combination with doxorubicin for 24 hours and cell viability was evaluated by using MTT assay. MTT assay showed a dose-dependent inhibition of cell proliferation with IC50 value of 37 µM. Brazilein increased doxorubicin’s cytotoxic activity on MCF-7/DOX cells. Both of single treatment with different concentration of brazilein 12.5 and 25 mM or doxorubicin 0.8 and 1 mM gave cell viability percentage above 80%, but combination of them led to decrease the cell viability percentage significantly. Based on this research, it can be concluded that brazilein is potential to be developed as a co-chemotherapy agent on breast cancer cell that have been resistant to doxorubicin. Futher study must be held to evaluate its molecular mechanism.
Keywords : brazilein, doxorubicin, MCF-7/DOX, cytotoxic. |
| first_indexed | 2024-04-12T10:50:38Z |
| format | Article |
| id | doaj.art-fb72d33f147e49f0a17abc14966806b3 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-12T10:50:38Z |
| publishDate | 2015-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-fb72d33f147e49f0a17abc14966806b32022-12-22T03:36:16ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892015-06-0162586310.14499/indonesianjcanchemoprev6iss2pp58-6387Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX CellsNi Putu Linda Laksmiani0Ratna Asmah Susidarti1Edy Meiyanto2Department of Pharmacy, Faculty of Mathematics and Science, Universitas Udayana, BaliFaculty of Pharmacy, Universitas Gadjah MadaFaculty of Pharmacy, Universitas Gadjah MadaBrazilein is a compound obtained in a large amount from the dried heartwood of Secang (Caesalpinia sappan L.). Brazilein has strong cytotoxic effect in several cancer cell lines. This research was designed to evaluate the cytotoxic effect of brazilein and its combination with a chemotherapy agent, doxorubicin on MCF-7/DOX breast cancer cells. In the cytotoxicity assay, MCF-7/DOX cells were cultured in the presence of brazilein solely and in combination with doxorubicin for 24 hours and cell viability was evaluated by using MTT assay. MTT assay showed a dose-dependent inhibition of cell proliferation with IC50 value of 37 µM. Brazilein increased doxorubicin’s cytotoxic activity on MCF-7/DOX cells. Both of single treatment with different concentration of brazilein 12.5 and 25 mM or doxorubicin 0.8 and 1 mM gave cell viability percentage above 80%, but combination of them led to decrease the cell viability percentage significantly. Based on this research, it can be concluded that brazilein is potential to be developed as a co-chemotherapy agent on breast cancer cell that have been resistant to doxorubicin. Futher study must be held to evaluate its molecular mechanism. Keywords : brazilein, doxorubicin, MCF-7/DOX, cytotoxic.https://ijcc.chemoprev.org/index.php/ijcc/article/view/86 |
| spellingShingle | Ni Putu Linda Laksmiani Ratna Asmah Susidarti Edy Meiyanto Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells |
| title_full | Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells |
| title_fullStr | Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells |
| title_full_unstemmed | Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells |
| title_short | Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells |
| title_sort | brazilein increased cytotoxic activity of doxorubicin on mcf 7 dox cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/86 |
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