Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain
The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the X...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2003-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200016 |
| _version_ | 1818585591531438080 |
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| author | Marcos Antonio de Morais Junior José Ferreira dos Santos João Antonio Pegas Henriques |
| author_facet | Marcos Antonio de Morais Junior José Ferreira dos Santos João Antonio Pegas Henriques |
| author_sort | Marcos Antonio de Morais Junior |
| collection | DOAJ |
| description | The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation. |
| first_indexed | 2024-12-16T08:39:30Z |
| format | Article |
| id | doaj.art-fb7872b4fdcf4971a5ac4796051f8201 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-16T08:39:30Z |
| publishDate | 2003-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-fb7872b4fdcf4971a5ac4796051f82012022-12-21T22:37:43ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-01-0126221322010.1590/S1415-47572003000200016Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strainMarcos Antonio de Morais JuniorJosé Ferreira dos SantosJoão Antonio Pegas HenriquesThe Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200016DNA repairgenetic recombinationrecA genesporulationyeast |
| spellingShingle | Marcos Antonio de Morais Junior José Ferreira dos Santos João Antonio Pegas Henriques Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain Genetics and Molecular Biology DNA repair genetic recombination recA gene sporulation yeast |
| title | Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain |
| title_full | Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain |
| title_fullStr | Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain |
| title_full_unstemmed | Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain |
| title_short | Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain |
| title_sort | expression of the bacterial reca gene impairs genetic recombination and sporulation in a saccharomyces cerevisiae diploid strain |
| topic | DNA repair genetic recombination recA gene sporulation yeast |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200016 |
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