Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets
Extracellular vesicles (EVs) are derived from the membrane of platelets and released in the circulation upon activation or injury. Analogous to the parent cell, platelet derived EVs play an important role in hemostasis and immune responses by transfer of bioactive cargo from the parent cells. Platel...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2023-05-01
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| Series: | Journal of Innate Immunity |
| Online Access: | https://beta.karger.com/Article/FullText/531266 |
| _version_ | 1797797638601441280 |
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| author | Frida Palm Axel Broman Genevieve Marcoux John W. Semple Thomas L Laurell Johan Malmström Oonagh Shannon |
| author_facet | Frida Palm Axel Broman Genevieve Marcoux John W. Semple Thomas L Laurell Johan Malmström Oonagh Shannon |
| author_sort | Frida Palm |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are derived from the membrane of platelets and released in the circulation upon activation or injury. Analogous to the parent cell, platelet derived EVs play an important role in hemostasis and immune responses by transfer of bioactive cargo from the parent cells. Platelet activation and release of EVs increases in several pathological inflammatory diseases, such as sepsis. We have previously reported that the M1 protein released from the bacterial pathogen Streptococcus pyogenes directly mediates platelet activation. In this study, EVs were isolated from these pathogen-activated platelets using acoustic trapping and their inflammation phenotype was characterized using quantitative mass spectrometry-based proteomics and cell-based models of inflammation. We determined that M1 protein mediated release of platelet derived EVs that contained the M1 protein. The isolated EVs derived from pathogen-activated platelets contained a similar protein cargo to those from physiologically activated platelets (thrombin), and included platelet membrane proteins, granule proteins and cytoskeletal proteins, coagulation factors and immune mediators. Immunomodulatory cargo, complement proteins and IgG3, were significantly enriched in EVs isolated from M1 protein-stimulated platelets. Acoustically enriched EVs were functionally intact and exhibited proinflammatory effects on addition to blood, including platelet-neutrophil complex formation, neutrophil activation, and cytokine release. Collectively, our findings reveal novel aspects of pathogen-mediated platelet activation during invasive streptococcal infection. |
| first_indexed | 2024-03-13T03:51:23Z |
| format | Article |
| id | doaj.art-fb7dc2fd5aee4bdc870b8a275b4a3844 |
| institution | Directory Open Access Journal |
| issn | 1662-811X 1662-8128 |
| language | English |
| last_indexed | 2024-03-13T03:51:23Z |
| publishDate | 2023-05-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Journal of Innate Immunity |
| spelling | doaj.art-fb7dc2fd5aee4bdc870b8a275b4a38442023-06-22T13:45:57ZengKarger PublishersJournal of Innate Immunity1662-811X1662-81282023-05-011110.1159/000531266531266Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated plateletsFrida PalmAxel Bromanhttps://orcid.org/0000-0002-2500-701XGenevieve MarcouxJohn W. Semplehttps://orcid.org/0000-0002-1510-0077Thomas L LaurellJohan MalmströmOonagh Shannonhttps://orcid.org/0000-0001-8291-8189Extracellular vesicles (EVs) are derived from the membrane of platelets and released in the circulation upon activation or injury. Analogous to the parent cell, platelet derived EVs play an important role in hemostasis and immune responses by transfer of bioactive cargo from the parent cells. Platelet activation and release of EVs increases in several pathological inflammatory diseases, such as sepsis. We have previously reported that the M1 protein released from the bacterial pathogen Streptococcus pyogenes directly mediates platelet activation. In this study, EVs were isolated from these pathogen-activated platelets using acoustic trapping and their inflammation phenotype was characterized using quantitative mass spectrometry-based proteomics and cell-based models of inflammation. We determined that M1 protein mediated release of platelet derived EVs that contained the M1 protein. The isolated EVs derived from pathogen-activated platelets contained a similar protein cargo to those from physiologically activated platelets (thrombin), and included platelet membrane proteins, granule proteins and cytoskeletal proteins, coagulation factors and immune mediators. Immunomodulatory cargo, complement proteins and IgG3, were significantly enriched in EVs isolated from M1 protein-stimulated platelets. Acoustically enriched EVs were functionally intact and exhibited proinflammatory effects on addition to blood, including platelet-neutrophil complex formation, neutrophil activation, and cytokine release. Collectively, our findings reveal novel aspects of pathogen-mediated platelet activation during invasive streptococcal infection.https://beta.karger.com/Article/FullText/531266 |
| spellingShingle | Frida Palm Axel Broman Genevieve Marcoux John W. Semple Thomas L Laurell Johan Malmström Oonagh Shannon Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets Journal of Innate Immunity |
| title | Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets |
| title_full | Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets |
| title_fullStr | Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets |
| title_full_unstemmed | Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets |
| title_short | Phenotypic characterization of acoustically enriched extracellular vesicles from pathogen-activated platelets |
| title_sort | phenotypic characterization of acoustically enriched extracellular vesicles from pathogen activated platelets |
| url | https://beta.karger.com/Article/FullText/531266 |
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