Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control
Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is one of the world's most serious diseases because it reduces yield and seed quality. Nowadays, biological control is an environment-friendly option for controlling plant diseases. The goals of this study were to (i) test eight end...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of the Saudi Society of Agricultural Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1658077X23000346 |
| _version_ | 1797653726386716672 |
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| author | Silvina Larran María Rosa Simón María Pilar Santamarina Josefa Roselló Caselles Verónica Fabiana Consolo Analía Perelló |
| author_facet | Silvina Larran María Rosa Simón María Pilar Santamarina Josefa Roselló Caselles Verónica Fabiana Consolo Analía Perelló |
| author_sort | Silvina Larran |
| collection | DOAJ |
| description | Charcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is one of the world's most serious diseases because it reduces yield and seed quality. Nowadays, biological control is an environment-friendly option for controlling plant diseases. The goals of this study were to (i) test eight endophytic Trichoderma spp. strains as biocontrol agents against M. phaseolina and (ii) further investigate two selected strains showing good behaviour against the pathogen. Pathogen-antagonist interactions were studied in dual culture, and the morphological alterations of M. phaseolina mycelia in the interaction zone were examined by light microscopy. Trichoderma strains were applied to soya bean seeds by a seed coating technique. Their bioprotective effects were assessed by in vitro and in vivo assays to evaluate radicle length, the germination percentage and the presence of typical charcoal rot symptoms in seedlings. Two Trichoderma strains were selected and they were molecularly identified as T. harzianum species complex. Their antagonistic ability against M. phaseolina was evaluated under different water availability conditions. The mechanisms used by these two endophytic strains against the pathogen were evaluated by cryo-scanning electron microscopy. The results showed that all eight Trichoderma strains successfully performed biocontrol activity against M. phaseolina by reducing colony growth and causing morphological alterations in the mycelia of M. phaseolina. All endophytes improved seed germination and radicle length, and reduced typical symptoms and disease progression on seedlings. Water availability in the medium impacted on fungal growth. At 0.995 ɑw, all the fungi grew more and faster. At 0.95 ɑw M. phaseolina grew more than the Trichoderma strains, while the pathogen grew slightly more at 0.98 ɑw than the Trichoderma strains. However, both selected Trichoderma strains grew larger and faster than the pathogen at 0.995 ɑw. The mechanisms involved in pathogen control revealed by the light and cryo-scanning microscopy studies included competition for nutrients or space and direct mycoparasitism. All the endophytic Trichoderma strains were antagonistic against M. phaseolina, however our study allowed us to select two Trichoderma strains with good potential to be included for charcoal rot management. |
| first_indexed | 2024-03-11T16:48:47Z |
| format | Article |
| id | doaj.art-fb812508fcba42e894f0df788ab3bda5 |
| institution | Directory Open Access Journal |
| issn | 1658-077X |
| language | English |
| last_indexed | 2024-03-11T16:48:47Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Saudi Society of Agricultural Sciences |
| spelling | doaj.art-fb812508fcba42e894f0df788ab3bda52023-10-22T04:48:23ZengElsevierJournal of the Saudi Society of Agricultural Sciences1658-077X2023-10-01227395406Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot controlSilvina Larran0María Rosa Simón1María Pilar Santamarina2Josefa Roselló Caselles3Verónica Fabiana Consolo4Analía Perelló5Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119 S/N, B1900 La Plata, Buenos Aires, Argentina; Corresponding author at: CIDEFI, Facultad de Ciencias Agrarias y Forestales, UNLP, Argentina.Cerealicultura, Departamento de Tecnología Agropecuaria y Forestal, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata. 60 y 119, CC 31, La Plata B1900, Buenos Aires, Argentina; CONICET Centro Científico Tecnológico La Plata, La Plata, ArgentinaDepartamento de Ecosistemas Agroforestales, Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural, Universidad Politécnica de Valencia, Camino de Vera, s/n, CP 46022 Valencia, EspañaDepartamento de Ecosistemas Agroforestales, Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural, Universidad Politécnica de Valencia, Camino de Vera, s/n, CP 46022 Valencia, EspañaInstituto de Investigaciones en Biodiversidad y Biotecnología – Fundación para Investigaciones Biológicas Aplicadas, Vieytes 3103, 7600 Mar del Plata, Buenos Aires, ArgentinaCentro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119 S/N, B1900 La Plata, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). 8 n° 1467, B1904 La Plata, Buenos Aires, ArgentinaCharcoal rot, caused by Macrophomina phaseolina (Tassi) Goid., is one of the world's most serious diseases because it reduces yield and seed quality. Nowadays, biological control is an environment-friendly option for controlling plant diseases. The goals of this study were to (i) test eight endophytic Trichoderma spp. strains as biocontrol agents against M. phaseolina and (ii) further investigate two selected strains showing good behaviour against the pathogen. Pathogen-antagonist interactions were studied in dual culture, and the morphological alterations of M. phaseolina mycelia in the interaction zone were examined by light microscopy. Trichoderma strains were applied to soya bean seeds by a seed coating technique. Their bioprotective effects were assessed by in vitro and in vivo assays to evaluate radicle length, the germination percentage and the presence of typical charcoal rot symptoms in seedlings. Two Trichoderma strains were selected and they were molecularly identified as T. harzianum species complex. Their antagonistic ability against M. phaseolina was evaluated under different water availability conditions. The mechanisms used by these two endophytic strains against the pathogen were evaluated by cryo-scanning electron microscopy. The results showed that all eight Trichoderma strains successfully performed biocontrol activity against M. phaseolina by reducing colony growth and causing morphological alterations in the mycelia of M. phaseolina. All endophytes improved seed germination and radicle length, and reduced typical symptoms and disease progression on seedlings. Water availability in the medium impacted on fungal growth. At 0.995 ɑw, all the fungi grew more and faster. At 0.95 ɑw M. phaseolina grew more than the Trichoderma strains, while the pathogen grew slightly more at 0.98 ɑw than the Trichoderma strains. However, both selected Trichoderma strains grew larger and faster than the pathogen at 0.995 ɑw. The mechanisms involved in pathogen control revealed by the light and cryo-scanning microscopy studies included competition for nutrients or space and direct mycoparasitism. All the endophytic Trichoderma strains were antagonistic against M. phaseolina, however our study allowed us to select two Trichoderma strains with good potential to be included for charcoal rot management.http://www.sciencedirect.com/science/article/pii/S1658077X23000346EndophytesTrichoderma spp.Biological controlMacrophomina phaseolinaSoya bean |
| spellingShingle | Silvina Larran María Rosa Simón María Pilar Santamarina Josefa Roselló Caselles Verónica Fabiana Consolo Analía Perelló Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control Journal of the Saudi Society of Agricultural Sciences Endophytes Trichoderma spp. Biological control Macrophomina phaseolina Soya bean |
| title | Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control |
| title_full | Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control |
| title_fullStr | Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control |
| title_full_unstemmed | Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control |
| title_short | Endophytic Trichoderma strains increase soya bean growth and promote charcoal rot control |
| title_sort | endophytic trichoderma strains increase soya bean growth and promote charcoal rot control |
| topic | Endophytes Trichoderma spp. Biological control Macrophomina phaseolina Soya bean |
| url | http://www.sciencedirect.com/science/article/pii/S1658077X23000346 |
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