Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat
<i>Fusarium</i> species can cause head blight of cereals worldwide. This is accompanied by impacts on yield and contamination of grains with mycotoxins. Regulations, with maximum limits, exist for the relevant <i>Fusarium</i> mycotoxins (e.g., type A and B trichothecenes, zea...
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/11/577 |
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| author | Esther Garcia-Cela Carol Verheecke-Vaessen Inga Ósk-Jónsdóttir Rita Lawson Naresh Magan |
| author_facet | Esther Garcia-Cela Carol Verheecke-Vaessen Inga Ósk-Jónsdóttir Rita Lawson Naresh Magan |
| author_sort | Esther Garcia-Cela |
| collection | DOAJ |
| description | <i>Fusarium</i> species can cause head blight of cereals worldwide. This is accompanied by impacts on yield and contamination of grains with mycotoxins. Regulations, with maximum limits, exist for the relevant <i>Fusarium</i> mycotoxins (e.g., type A and B trichothecenes, zearalenone and fumonisins). There is interest in a better understanding of the effect of key interacting abiotic factors which determine colonization and mycotoxin production in small grain cereals. Thus, this study examined the ecophysiological relationship between temperature and water availability (10–35 °C; water activity, a<sub>w</sub>, 0.87–0.98) on growth and production of <i>Fusarium</i> mycotoxins (zearelenone, ZEA; deoxynivalenol, DON; 3-acetyl deoxynivalenol, 3-Ac-DON and nivalenol, NIV) by three strains of <i>F. asiaticum,</i> a head blight pathogen isolated from China and becoming important in other global regions. These were carried out on simulated wheat-based matrices that identified the optimum (25 °C/0.98 a<sub>w</sub>) and marginal boundary conditions for growth (35 °C/0.90 a<sub>w</sub>) for all three strains. Contrarily, different mycotoxigenic profiles were observed between strains (<i>p</i> < 0.05). Four mycotoxins assessed were produced at 30 °C while cold temperature inhibited the production of NIV and ZEA, which were never detected at <20 and <15 °C, respectively. Optimal mycotoxin production conditions varied for each toxin with ZEA production which was best at 30 °C/0.93–0.95 a<sub>w</sub>, DON, 3-Ac-DON and NIV which was 0.98 a<sub>w</sub>/20–30 °C. Probabilistic models were used to predict growth and regulated mycotoxin production by the strains of <i>F. asiaticum</i>. This study will be beneficial in the development mitigation strategies for control of pre- and post-harvest colonization of cereals and mycotoxin contamination by this <i>Fusarium</i> species in cereals. |
| first_indexed | 2024-03-09T19:06:01Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T19:06:01Z |
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| spelling | doaj.art-fb828282ab424fcda43559c5e6049a602023-11-24T04:37:42ZengMDPI AGFermentation2311-56372022-10-0181157710.3390/fermentation8110577Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from WheatEsther Garcia-Cela0Carol Verheecke-Vaessen1Inga Ósk-Jónsdóttir2Rita Lawson3Naresh Magan4Applied Mycology Group, Environment AgriFood Theme, Cranfield University, Cranfield MK43 0AL, UKApplied Mycology Group, Environment AgriFood Theme, Cranfield University, Cranfield MK43 0AL, UKApplied Mycology Group, Environment AgriFood Theme, Cranfield University, Cranfield MK43 0AL, UKApplied Mycology Group, Environment AgriFood Theme, Cranfield University, Cranfield MK43 0AL, UKApplied Mycology Group, Environment AgriFood Theme, Cranfield University, Cranfield MK43 0AL, UK<i>Fusarium</i> species can cause head blight of cereals worldwide. This is accompanied by impacts on yield and contamination of grains with mycotoxins. Regulations, with maximum limits, exist for the relevant <i>Fusarium</i> mycotoxins (e.g., type A and B trichothecenes, zearalenone and fumonisins). There is interest in a better understanding of the effect of key interacting abiotic factors which determine colonization and mycotoxin production in small grain cereals. Thus, this study examined the ecophysiological relationship between temperature and water availability (10–35 °C; water activity, a<sub>w</sub>, 0.87–0.98) on growth and production of <i>Fusarium</i> mycotoxins (zearelenone, ZEA; deoxynivalenol, DON; 3-acetyl deoxynivalenol, 3-Ac-DON and nivalenol, NIV) by three strains of <i>F. asiaticum,</i> a head blight pathogen isolated from China and becoming important in other global regions. These were carried out on simulated wheat-based matrices that identified the optimum (25 °C/0.98 a<sub>w</sub>) and marginal boundary conditions for growth (35 °C/0.90 a<sub>w</sub>) for all three strains. Contrarily, different mycotoxigenic profiles were observed between strains (<i>p</i> < 0.05). Four mycotoxins assessed were produced at 30 °C while cold temperature inhibited the production of NIV and ZEA, which were never detected at <20 and <15 °C, respectively. Optimal mycotoxin production conditions varied for each toxin with ZEA production which was best at 30 °C/0.93–0.95 a<sub>w</sub>, DON, 3-Ac-DON and NIV which was 0.98 a<sub>w</sub>/20–30 °C. Probabilistic models were used to predict growth and regulated mycotoxin production by the strains of <i>F. asiaticum</i>. This study will be beneficial in the development mitigation strategies for control of pre- and post-harvest colonization of cereals and mycotoxin contamination by this <i>Fusarium</i> species in cereals.https://www.mdpi.com/2311-5637/8/11/577predictive modelprobabilistic modelwheatbarleytrichotheceneszearalenone |
| spellingShingle | Esther Garcia-Cela Carol Verheecke-Vaessen Inga Ósk-Jónsdóttir Rita Lawson Naresh Magan Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat Fermentation predictive model probabilistic model wheat barley trichothecenes zearalenone |
| title | Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat |
| title_full | Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat |
| title_fullStr | Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat |
| title_full_unstemmed | Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat |
| title_short | Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three <i>Fusarium asiaticum</i> Strains Isolated from Wheat |
| title_sort | growth kinetic parameters and prediction of growth and zearalenone and deoxynivalenol production boundaries by three i fusarium asiaticum i strains isolated from wheat |
| topic | predictive model probabilistic model wheat barley trichothecenes zearalenone |
| url | https://www.mdpi.com/2311-5637/8/11/577 |
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