Diagnostic and prognostic value of IDH1 mutation in gliomas
Objective To discuss the significance of IDH1 mutation for diagnosis and prognosis of gliomas through detecting of IDH1 mutation in supratentorial glioma cells. Methods IDH1 genes of postoperative pathological samples obtained from 315 patients with supratentorial gliomas (3 cases of WHO Ⅰ, 95 cases...
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| Format: | Article |
| Language: | English |
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Tianjin Huanhu Hospital
2012-12-01
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| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
| Subjects: | |
| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/626 |
| _version_ | 1823992609496891392 |
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| author | Yan⁃wu YANG Fei XIE Jun⁃jia TANG Shuang LIU Xiang WANG Yan⁃hui LIU Qing MAO |
| author_facet | Yan⁃wu YANG Fei XIE Jun⁃jia TANG Shuang LIU Xiang WANG Yan⁃hui LIU Qing MAO |
| author_sort | Yan⁃wu YANG |
| collection | DOAJ |
| description | Objective To discuss the significance of IDH1 mutation for diagnosis and prognosis of gliomas through detecting of IDH1 mutation in supratentorial glioma cells. Methods IDH1 genes of postoperative pathological samples obtained from 315 patients with supratentorial gliomas (3 cases of WHO Ⅰ, 95 cases of WHO Ⅱ, 37 cases of WHO Ⅲ and 180 cases of WHO Ⅳ) were collected for DNA extraction, on which PCR amplification and direct sequencing were done. At the same time, multiple⁃factor analysis was used on those patients' age, sex, tumor location, excision extension, Karnofsky Performance Status (KPS) and progression⁃free survival time or median survival time, to investigate the influence of IDH1 mutation on the prognosis of patients. Results A total of 112 gene mutations among 315 cases (35.56%) were found and they were all R132H type of mutations. Mutation rates in WHOⅡ, Ⅲ and Ⅳ gliomas were 72.63% (69/95), 24.32% (9/37) and 18.89% (34/180) respectively, which were significantly different (P = 0.000, for all). Among patients with IDH1 mutations in WHOⅣ gliomas, there were 18 cases of primary glioblastoma and 16 cases of secondary glioblastoma, the mutation rates of which were 11.39% (18/158) and 72.73% (16/22), respectively. The latter was much higher than the former and the difference was statistically significant ( χ 2 = 23.654，P = 0.001). Survival analysis revealed that IDH1 mutation presented notable effect on the prognosis of patients with gliomas. Conclusion IDH1 mutation can happen in WHO Ⅱ, Ⅲ and Ⅳ gliomas, and the mutation rates of WHOⅡ gliomas and secondary glioblastomas of WHO Ⅳ are higher than others. The survival time of patients are remarkably influenced by IDH1 mutation, and the prognosis of patient with IDH1 mutation is good, which may suggest that IDH1 mutation is an important diagnostic and prognostic biomarker in different grades of gliomas．
DOI：10.3969/j.issn.1672⁃6731.2012.06.015 |
| first_indexed | 2024-12-18T16:10:23Z |
| format | Article |
| id | doaj.art-fb83bd6ca66b4715aff72f9e8c3eb0f0 |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-12-18T16:10:23Z |
| publishDate | 2012-12-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-fb83bd6ca66b4715aff72f9e8c3eb0f02022-12-21T21:02:06ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312012-12-01126712718625Diagnostic and prognostic value of IDH1 mutation in gliomasYan⁃wu YANGFei XIEJun⁃jia TANGShuang LIUXiang WANGYan⁃hui LIUQing MAOObjective To discuss the significance of IDH1 mutation for diagnosis and prognosis of gliomas through detecting of IDH1 mutation in supratentorial glioma cells. Methods IDH1 genes of postoperative pathological samples obtained from 315 patients with supratentorial gliomas (3 cases of WHO Ⅰ, 95 cases of WHO Ⅱ, 37 cases of WHO Ⅲ and 180 cases of WHO Ⅳ) were collected for DNA extraction, on which PCR amplification and direct sequencing were done. At the same time, multiple⁃factor analysis was used on those patients' age, sex, tumor location, excision extension, Karnofsky Performance Status (KPS) and progression⁃free survival time or median survival time, to investigate the influence of IDH1 mutation on the prognosis of patients. Results A total of 112 gene mutations among 315 cases (35.56%) were found and they were all R132H type of mutations. Mutation rates in WHOⅡ, Ⅲ and Ⅳ gliomas were 72.63% (69/95), 24.32% (9/37) and 18.89% (34/180) respectively, which were significantly different (P = 0.000, for all). Among patients with IDH1 mutations in WHOⅣ gliomas, there were 18 cases of primary glioblastoma and 16 cases of secondary glioblastoma, the mutation rates of which were 11.39% (18/158) and 72.73% (16/22), respectively. The latter was much higher than the former and the difference was statistically significant ( χ 2 = 23.654，P = 0.001). Survival analysis revealed that IDH1 mutation presented notable effect on the prognosis of patients with gliomas. Conclusion IDH1 mutation can happen in WHO Ⅱ, Ⅲ and Ⅳ gliomas, and the mutation rates of WHOⅡ gliomas and secondary glioblastomas of WHO Ⅳ are higher than others. The survival time of patients are remarkably influenced by IDH1 mutation, and the prognosis of patient with IDH1 mutation is good, which may suggest that IDH1 mutation is an important diagnostic and prognostic biomarker in different grades of gliomas． DOI：10.3969/j.issn.1672⁃6731.2012.06.015http://www.cjcnn.org/index.php/cjcnn/article/view/626GenesMutationSupratentorial neoplasmsDiagnosisGliomaPrognosis |
| spellingShingle | Yan⁃wu YANG Fei XIE Jun⁃jia TANG Shuang LIU Xiang WANG Yan⁃hui LIU Qing MAO Diagnostic and prognostic value of IDH1 mutation in gliomas Chinese Journal of Contemporary Neurology and Neurosurgery Genes Mutation Supratentorial neoplasms Diagnosis Glioma Prognosis |
| title | Diagnostic and prognostic value of IDH1 mutation in gliomas |
| title_full | Diagnostic and prognostic value of IDH1 mutation in gliomas |
| title_fullStr | Diagnostic and prognostic value of IDH1 mutation in gliomas |
| title_full_unstemmed | Diagnostic and prognostic value of IDH1 mutation in gliomas |
| title_short | Diagnostic and prognostic value of IDH1 mutation in gliomas |
| title_sort | diagnostic and prognostic value of idh1 mutation in gliomas |
| topic | Genes Mutation Supratentorial neoplasms Diagnosis Glioma Prognosis |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/626 |
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