Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System
Osteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. More...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/12/10/2203 |
| _version_ | 1797552591380414464 |
|---|---|
| author | JunJie Yu SuJeong Lee Sunkyung Choi Kee K. Kim Bokyeong Ryu C-Yoon Kim Cho-Rok Jung Byoung-Hyun Min Yuan-Zhu Xin Su A Park Wandoo Kim Donghyun Lee JunHee Lee |
| author_facet | JunJie Yu SuJeong Lee Sunkyung Choi Kee K. Kim Bokyeong Ryu C-Yoon Kim Cho-Rok Jung Byoung-Hyun Min Yuan-Zhu Xin Su A Park Wandoo Kim Donghyun Lee JunHee Lee |
| author_sort | JunJie Yu |
| collection | DOAJ |
| description | Osteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. Moreover, there is a great need for a valuable in vitro platform enabling the assessment of osteochondral tissues to reduce pre-operative risk. Three-dimensional (3D) bioprinting systems may be a promising approach for fabricating human tissues and organs. Here, we aimed to develop a polycaprolactone (PCL)/alginate bipartite hybrid scaffold using a multihead 3D bioprinting system. The hybrid scaffold was composed of PCL, which could improve the mechanical properties of the construct, and alginate, encapsulating progenitor cells that could differentiate into cartilage and bone. To differentiate the bipartite hybrid scaffold into osteochondral tissue, a polydimethylsiloxane coculture system for osteochondral tissue (PCSOT) was designed and developed. Based on evaluation of the biological performance of the novel hybrid scaffold, the PCL/alginate bipartite scaffold was successfully fabricated; importantly, our findings suggest that this PCSOT system may be applicable as an in vitro platform for osteochondral tissue engineering. |
| first_indexed | 2024-03-10T16:03:17Z |
| format | Article |
| id | doaj.art-fb83d9ba30ed49699dcc9c0430fd7adc |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T16:03:17Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-fb83d9ba30ed49699dcc9c0430fd7adc2023-11-20T15:10:01ZengMDPI AGPolymers2073-43602020-09-011210220310.3390/polym12102203Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting SystemJunJie Yu0SuJeong Lee1Sunkyung Choi2Kee K. Kim3Bokyeong Ryu4C-Yoon Kim5Cho-Rok Jung6Byoung-Hyun Min7Yuan-Zhu Xin8Su A Park9Wandoo Kim10Donghyun Lee11JunHee Lee12Department of Nature-Inspired System and Application, Korea Institute of Machinery & Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaMedical Device Convergence Center, Konyang University Hospital, 158 Gwanjedong-Ro, Seo-Gu, Daejeon 35365, KoreaDepartment of Biochemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Biochemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, KoreaDepartment of Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, KoreaKorea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-Gu, Daejeon 34141, KoreaDepartment of Orthopedic Surgery, School of Medicine, Ajou University, 206 World Cup-ro, Yeongtonggu, Suwon 16499, KoreaDepartment of Engineering Mechanics, School of Mechanical and Aerospace Engineering, Jilin University, No. 5988, Renmin Street, Changchun 130025, ChinaDepartment of Nature-Inspired System and Application, Korea Institute of Machinery & Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaDepartment of Nature-Inspired System and Application, Korea Institute of Machinery & Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaDepartment of Biomedical Engineering, School of Integrative Engineering, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, KoreaDepartment of Nature-Inspired System and Application, Korea Institute of Machinery & Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaOsteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. Moreover, there is a great need for a valuable in vitro platform enabling the assessment of osteochondral tissues to reduce pre-operative risk. Three-dimensional (3D) bioprinting systems may be a promising approach for fabricating human tissues and organs. Here, we aimed to develop a polycaprolactone (PCL)/alginate bipartite hybrid scaffold using a multihead 3D bioprinting system. The hybrid scaffold was composed of PCL, which could improve the mechanical properties of the construct, and alginate, encapsulating progenitor cells that could differentiate into cartilage and bone. To differentiate the bipartite hybrid scaffold into osteochondral tissue, a polydimethylsiloxane coculture system for osteochondral tissue (PCSOT) was designed and developed. Based on evaluation of the biological performance of the novel hybrid scaffold, the PCL/alginate bipartite scaffold was successfully fabricated; importantly, our findings suggest that this PCSOT system may be applicable as an in vitro platform for osteochondral tissue engineering.https://www.mdpi.com/2073-4360/12/10/2203progenitor cellthree-dimensional bioprinting systemhybrid scaffoldosteochondral tissue |
| spellingShingle | JunJie Yu SuJeong Lee Sunkyung Choi Kee K. Kim Bokyeong Ryu C-Yoon Kim Cho-Rok Jung Byoung-Hyun Min Yuan-Zhu Xin Su A Park Wandoo Kim Donghyun Lee JunHee Lee Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System Polymers progenitor cell three-dimensional bioprinting system hybrid scaffold osteochondral tissue |
| title | Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System |
| title_full | Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System |
| title_fullStr | Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System |
| title_full_unstemmed | Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System |
| title_short | Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System |
| title_sort | fabrication of a polycaprolactone alginate bipartite hybrid scaffold for osteochondral tissue using a three dimensional bioprinting system |
| topic | progenitor cell three-dimensional bioprinting system hybrid scaffold osteochondral tissue |
| url | https://www.mdpi.com/2073-4360/12/10/2203 |
| work_keys_str_mv | AT junjieyu fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT sujeonglee fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT sunkyungchoi fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT keekkim fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT bokyeongryu fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT cyoonkim fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT chorokjung fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT byounghyunmin fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT yuanzhuxin fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT suapark fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT wandookim fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT donghyunlee fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem AT junheelee fabricationofapolycaprolactonealginatebipartitehybridscaffoldforosteochondraltissueusingathreedimensionalbioprintingsystem |