Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers
Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/9/11/734 |
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| author | Bruce R. Robinson Carolina Garcia Salinas Perla Ramos Parra John Bamberg Rocio I. Diaz de la Garza Aymeric Goyer |
| author_facet | Bruce R. Robinson Carolina Garcia Salinas Perla Ramos Parra John Bamberg Rocio I. Diaz de la Garza Aymeric Goyer |
| author_sort | Bruce R. Robinson |
| collection | DOAJ |
| description | Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a <i>Solanum boliviense</i> Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers. |
| first_indexed | 2024-12-17T23:02:02Z |
| format | Article |
| id | doaj.art-fb85e441d0554c27906194f00a53e4df |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-17T23:02:02Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-fb85e441d0554c27906194f00a53e4df2022-12-21T21:29:23ZengMDPI AGAgronomy2073-43952019-11-0191173410.3390/agronomy9110734agronomy9110734Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato TubersBruce R. Robinson0Carolina Garcia Salinas1Perla Ramos Parra2John Bamberg3Rocio I. Diaz de la Garza4Aymeric Goyer5Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USATecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, Nuevo León, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, Nuevo León, MexicoUSDA/Agricultural Research Service, Sturgeon Bay, WI 54235, USATecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, Nuevo León, MexicoDepartment of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USABiofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a <i>Solanum boliviense</i> Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.https://www.mdpi.com/2073-4395/9/11/734folateregulationpotato |
| spellingShingle | Bruce R. Robinson Carolina Garcia Salinas Perla Ramos Parra John Bamberg Rocio I. Diaz de la Garza Aymeric Goyer Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers Agronomy folate regulation potato |
| title | Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers |
| title_full | Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers |
| title_fullStr | Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers |
| title_full_unstemmed | Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers |
| title_short | Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B<sub>9</sub> Content in Potato Tubers |
| title_sort | expression levels of the γ glutamyl hydrolase i gene predict vitamin b sub 9 sub content in potato tubers |
| topic | folate regulation potato |
| url | https://www.mdpi.com/2073-4395/9/11/734 |
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