Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals
Avian influenza viruses (AIVs) have the potential for cross-species transmission and pandemics. In recent years, clade 2.3.4.4 H5N6 AIVs are prevalent in domestic poultry, posing a threat to the domestic poultry industry and public health. In this study, two strains of H5N6 AIVs were isolated from c...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/22/3079 |
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| author | Cheng Zhang Huan Cui Chunmao Zhang Kui Zhao Yunyi Kong Ligong Chen Shishan Dong Zhaoliang Chen Jie Pu Lei Zhang Zhendong Guo Juxiang Liu |
| author_facet | Cheng Zhang Huan Cui Chunmao Zhang Kui Zhao Yunyi Kong Ligong Chen Shishan Dong Zhaoliang Chen Jie Pu Lei Zhang Zhendong Guo Juxiang Liu |
| author_sort | Cheng Zhang |
| collection | DOAJ |
| description | Avian influenza viruses (AIVs) have the potential for cross-species transmission and pandemics. In recent years, clade 2.3.4.4 H5N6 AIVs are prevalent in domestic poultry, posing a threat to the domestic poultry industry and public health. In this study, two strains of H5N6 AIVs were isolated from chickens in Hebei, China, in 2019: A/chicken/Hebei/HB1907/2019(H5N6) and A/chicken/Hebei/HB1905/2019(H5N6). Phylogenetic analysis showed that both viral HA genes clustered in the 2.3.4.4h clade. Receptor binding analysis showed that the HB1905 strain preferentially binds to α-2,3-linked sialic acid (SA) receptors, while the HB1907 strain preferentially binds to α-2,3- and α-2,6-linked sialic acid (SA) receptors. During early infection, the HB1907 strain is highly replicable in MDCK cells, more so than the HB1905 strain. Pathogenicity assays in mice showed that both viruses could replicate in the lungs without prior adaptation, with HB1907 being more highly pathogenic in mice than the HB1905 strain. Significantly, both the HB1905 and HB1907 strains can be transmitted through direct contact among guinea pigs, but the transmission efficiency of the HB1907 strain through contact between guinea pigs is much greater than that of the HB1905 strain. These results strengthen the need for ongoing surveillance and early warning of H5N6 AIVs in poultry. |
| first_indexed | 2024-03-09T18:32:47Z |
| format | Article |
| id | doaj.art-fb89e67d0ed3483387e9ca9b474a320f |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T18:32:47Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-fb89e67d0ed3483387e9ca9b474a320f2023-11-24T07:27:57ZengMDPI AGAnimals2076-26152022-11-011222307910.3390/ani12223079Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in MammalsCheng Zhang0Huan Cui1Chunmao Zhang2Kui Zhao3Yunyi Kong4Ligong Chen5Shishan Dong6Zhaoliang Chen7Jie Pu8Lei Zhang9Zhendong Guo10Juxiang Liu11College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaCollege of Animal Medicine, Jilin University, Changchun 130062, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun 130122, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, ChinaAvian influenza viruses (AIVs) have the potential for cross-species transmission and pandemics. In recent years, clade 2.3.4.4 H5N6 AIVs are prevalent in domestic poultry, posing a threat to the domestic poultry industry and public health. In this study, two strains of H5N6 AIVs were isolated from chickens in Hebei, China, in 2019: A/chicken/Hebei/HB1907/2019(H5N6) and A/chicken/Hebei/HB1905/2019(H5N6). Phylogenetic analysis showed that both viral HA genes clustered in the 2.3.4.4h clade. Receptor binding analysis showed that the HB1905 strain preferentially binds to α-2,3-linked sialic acid (SA) receptors, while the HB1907 strain preferentially binds to α-2,3- and α-2,6-linked sialic acid (SA) receptors. During early infection, the HB1907 strain is highly replicable in MDCK cells, more so than the HB1905 strain. Pathogenicity assays in mice showed that both viruses could replicate in the lungs without prior adaptation, with HB1907 being more highly pathogenic in mice than the HB1905 strain. Significantly, both the HB1905 and HB1907 strains can be transmitted through direct contact among guinea pigs, but the transmission efficiency of the HB1907 strain through contact between guinea pigs is much greater than that of the HB1905 strain. These results strengthen the need for ongoing surveillance and early warning of H5N6 AIVs in poultry.https://www.mdpi.com/2076-2615/12/22/3079chickensavian influenzaH5N6 subtypepathogenicitytransmissibility |
| spellingShingle | Cheng Zhang Huan Cui Chunmao Zhang Kui Zhao Yunyi Kong Ligong Chen Shishan Dong Zhaoliang Chen Jie Pu Lei Zhang Zhendong Guo Juxiang Liu Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals Animals chickens avian influenza H5N6 subtype pathogenicity transmissibility |
| title | Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals |
| title_full | Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals |
| title_fullStr | Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals |
| title_full_unstemmed | Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals |
| title_short | Pathogenicity and Transmissibility of Clade 2.3.4.4h H5N6 Avian Influenza Viruses in Mammals |
| title_sort | pathogenicity and transmissibility of clade 2 3 4 4h h5n6 avian influenza viruses in mammals |
| topic | chickens avian influenza H5N6 subtype pathogenicity transmissibility |
| url | https://www.mdpi.com/2076-2615/12/22/3079 |
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