Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels
We proposed gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels. To realize artificial 3D tissue, capillary blood vessels are important to be built in it as one of the typical small diameter cellular vessels. As previous works, a method to fabricat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Japanese |
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The Japan Society of Mechanical Engineers
2016-01-01
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| Series: | Nihon Kikai Gakkai ronbunshu |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/transjsme/82/833/82_15-00547/_pdf/-char/en |
| _version_ | 1797989139593822208 |
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| author | Natsuki TAKEI Masahiro NAKAJIMA Masaru TAKEUCHI Toshio FUKUDA |
| author_facet | Natsuki TAKEI Masahiro NAKAJIMA Masaru TAKEUCHI Toshio FUKUDA |
| author_sort | Natsuki TAKEI |
| collection | DOAJ |
| description | We proposed gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels. To realize artificial 3D tissue, capillary blood vessels are important to be built in it as one of the typical small diameter cellular vessels. As previous works, a method to fabricate patient-specific vascular scaffolds was proposed using a biodegradable polymer, PLCL (poly(L-lactide-co-ε-caprolactone)). In this paper, we demonstrated a way for seeding smooth muscle cells and fibroblast cells outside the PLCL scaffold using the gel-fibers reeling system. At first, the mechanical stiffness of gel fibers was determined to achieve fiber reeling. The tensile stress of gel fibers was also evaluated without breaking down. The reeling speed of gel fiber was determined for seeding cells uniformly on a scaffold. The multiple hydrogel fibers embedding biological cells were reeled onto tubular PLCL scaffold, and then the alginate gel were melted by an alginate lyase. A uniform cell seeding onto upper and under surfaces of PLCL scaffold was achieved by rotating scaffold at 180 degrees every one hour. As a result, we achieved seeding cells onto scaffold and the cells viability was more than 90%. The proposed gel-fibers reeling system is important to be applicable in wide areas such as tissue engineering and bio simulators. |
| first_indexed | 2024-04-11T08:15:26Z |
| format | Article |
| id | doaj.art-fb8ab9a6b5d148cfb00798f531ca6508 |
| institution | Directory Open Access Journal |
| issn | 2187-9761 |
| language | Japanese |
| last_indexed | 2024-04-11T08:15:26Z |
| publishDate | 2016-01-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Nihon Kikai Gakkai ronbunshu |
| spelling | doaj.art-fb8ab9a6b5d148cfb00798f531ca65082022-12-22T04:35:11ZjpnThe Japan Society of Mechanical EngineersNihon Kikai Gakkai ronbunshu2187-97612016-01-018283315-0054715-0054710.1299/transjsme.15-00547transjsmeGel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vesselsNatsuki TAKEI0Masahiro NAKAJIMA1Masaru TAKEUCHI2Toshio FUKUDA3Graduate School of Engineering, Nagoya UniversityGraduate School of Engineering, Nagoya UniversityGraduate School of Engineering, Nagoya UniversityInstitute of Advanced Research, Nagoya UniversityWe proposed gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels. To realize artificial 3D tissue, capillary blood vessels are important to be built in it as one of the typical small diameter cellular vessels. As previous works, a method to fabricate patient-specific vascular scaffolds was proposed using a biodegradable polymer, PLCL (poly(L-lactide-co-ε-caprolactone)). In this paper, we demonstrated a way for seeding smooth muscle cells and fibroblast cells outside the PLCL scaffold using the gel-fibers reeling system. At first, the mechanical stiffness of gel fibers was determined to achieve fiber reeling. The tensile stress of gel fibers was also evaluated without breaking down. The reeling speed of gel fiber was determined for seeding cells uniformly on a scaffold. The multiple hydrogel fibers embedding biological cells were reeled onto tubular PLCL scaffold, and then the alginate gel were melted by an alginate lyase. A uniform cell seeding onto upper and under surfaces of PLCL scaffold was achieved by rotating scaffold at 180 degrees every one hour. As a result, we achieved seeding cells onto scaffold and the cells viability was more than 90%. The proposed gel-fibers reeling system is important to be applicable in wide areas such as tissue engineering and bio simulators.https://www.jstage.jst.go.jp/article/transjsme/82/833/82_15-00547/_pdf/-char/ensmall diamter cellular vesselstissue engineering3d scaffoldgel fibers |
| spellingShingle | Natsuki TAKEI Masahiro NAKAJIMA Masaru TAKEUCHI Toshio FUKUDA Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels Nihon Kikai Gakkai ronbunshu small diamter cellular vessels tissue engineering 3d scaffold gel fibers |
| title | Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels |
| title_full | Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels |
| title_fullStr | Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels |
| title_full_unstemmed | Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels |
| title_short | Gel-fibers reeling system (Gel-FRS) with embedding biological cells for small diameter cellular vessels |
| title_sort | gel fibers reeling system gel frs with embedding biological cells for small diameter cellular vessels |
| topic | small diamter cellular vessels tissue engineering 3d scaffold gel fibers |
| url | https://www.jstage.jst.go.jp/article/transjsme/82/833/82_15-00547/_pdf/-char/en |
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